Synthesis and Secretion of Monocyte Chemotactic Protein-1 Stimulated by the High Affinity Receptor for IgE

Eglite, Santa; Morin, Juan M.; Metzger, Henry

doi:10.4049/jimmunol.170.5.2680

Cited by 16 publications

(12 citation statements)

References 27 publications

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“…We investigated the effect of the flavonoids on the release of CCL2 because it is known to be synthesized by MC 13 and it stimules recruitment of immune cells to the site of inflammation.…”

Section: Resultsmentioning

confidence: 99%

“…While luteolin had been reported to inhibit histamine release from hCBMCs 30 this is the first time that lut and methlut are reported to inhibit CCL2 release. CCL2 is known to be synthesized by MC 13 and stimulates recruitment of immune cells to the site of inflammation.…”

Section: Discussionmentioning

confidence: 99%

“…8;9 MC is probably the only cell type that stores preformed TNF, 10 which is rapidly released and influences T cell recruitment and activation. 11;12 MC-derived CCL2 13 and CXCL-8 ( IL-8) enhance recruitment of immune cells to the site of inflammation. 6;7;14 The ability to release multiple mediators allows MC to actively interact with other cell types in their surrounding environment, and participate in the induction and/or propagation of various immune and inflammatory responses including mastocytosis, 15 asthma 16 , atopic dermatitis, 17 and psoriasis.…”

Section: Introductionmentioning

confidence: 99%

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The novel flavone tetramethoxyluteolin is a potent inhibitor of human mast cells

Weng

Patel

Panagiotidou

et al. 2015

Journal of Allergy and Clinical Immunology

121

114

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Background Mast cells (MCs) are hemopoietic cells that mature in tissues and are involved in allergy, immunity and inflammation by secreting multiple mediators. The natural flavone luteolin (lut) has anti-inflammatory actions and inhibits human MCs. Objective To investigate the ability of lut, and its novel structural analog 3’,4’,5,7-tetramethoxyluteolin (methlut), to inhibit human MCs mediator expression and release in vitro and in vivo. Methods Human LAD2 cells and primary human umbilical cord-blood derived cultured MC (hCBMCs) were stimulated by substance P (SP) or IgE/anti-IgE with or without pre-incubation with lut, methlut or cromolyn (1–100 μM) for 2 or 24 hr following which a mediator secretion was measured. The effect of the compound on MC intracellular calcium levels and NF-κB activation was also investigated. Pretreatment with methlut was also studied in mice passively sensitized with dinotrophenol-human serum albumin (DNP-HSA) and challenged intradermally. Results Methlut is a more potent inhibitor than lut or cromolyn for beta-hexosaminidase (β-hex) and histamine secretion from LAD2 cells stimulated by either SP or IgE/anti-IgE, but only methlut and lut significantly inhibit preformed tumor necrosis factor (TNF) secretion. Methlut is also a more potent inhibitor than lut of de novo synthesized TNF from LAD2, and of chemokine (C-C motif) ligand 2 (CCL2) from hCBMCs. The mechanism of action from methlut may be due to its ability to inhibit intracellular calcium increase, as well as NF-κB induction at both the transcriptional and translational levels in LAD2 cells stimulated by SP without affecting cell viability. Treatment (ip) with methlut significantly decreases skin vascular permeability of Evans blue in mice passively sensitized to DNP-HAS and challenged intradermaly. Conclusion Methlut is a promising MC inhibitor for the treatment of allergic and inflammatory conditions.

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“…We investigated the effect of the flavonoids on the release of CCL2 because it is known to be synthesized by MC 13 and it stimules recruitment of immune cells to the site of inflammation.…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The novel flavone tetramethoxyluteolin is a potent inhibitor of human mast cells

Weng

Patel

Panagiotidou

et al. 2015

Journal of Allergy and Clinical Immunology

121

114

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“…The results showed that abnormal increases in IgE levels after treatment of TMA were significantly suppressed by oral administration of PFE, whereas PS treatment had no such effect ( Figure 3A). MCP-1 synthesis and secretion has been found to be closely related to IgE expression (Eglite et al, 2003). Thus, we sought to determine whether PFE affects MCP-1 expression.…”

Section: Effect Of Pfe On Tma-induced Ige Mmcp-1 and Il-1β Productimentioning

confidence: 99%

Piper nigrum Fruit Extract Prevents TMA-Induced Allergic Contact Dermatitis by Regulating Th2 Cytokine Production

Jung¹,

Choi²,

Jung³

et al. 2015

JAS

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Allergic contact dermatitis (ACD) remains a leading skin disease in many countries. In this study, we investigated the preventive effect of Piper nigrum fruit extract (PFE) on trimellitic anhydride (TMA)-induced dermatitis and its potential mechanism of action. Oral administration of PFE and prednisolone (PS) significantly suppressed TMA-induced increases in ear, epidermal thickness, and infiltration of CD4 + cells, while abnormal expression of IgE, mMCP-1, IL-1β and IL-1β mRNA was also significantly counteracted by oral administration of PFE. PFE also significantly suppresses TMA-induced IL-4 and IL-5 production and IL-4 mRNA expression in vivo, as well as OVA-induced IL-4, IL-5, and IL-13 production and GATA3 mRNA expression ex vivo, and IL-4 induced STAT6 phosphorylation in primary cultured splenocytes and HaCaT cells. Interestingly, the PFE component piperine significantly suppressed OVA-induced IL-4, IL-5, and IL-13 secretion ex vivo. Taken together, these results suggest that PFE could be useful in suppressing allergic contact dermatitis.

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“…A model explaining this escape has been proposed that involves the formation of a messenger that spreads the signal beyond the initial signaling complex containing the ligand and receptor (8,9). In the case of MCP-1, a gene for which transcriptional activation appears to escape kinetic proofreading in RBL-2H3 cells, several experiments have been interpreted as supporting the hypothesis that calcium ions (Ca 2ϩ ) released from intracellular stores may serve as the messenger that allows escape (10). Transcriptional activation of other chemokines also appears to escape kinetic proofreading through similar mechanisms (6).…”

mentioning

confidence: 98%

Kinetic Proofreading of Ligand-FcεRI Interactions May Persist beyond LAT Phosphorylation

Torigoe

Faeder

Oliver

et al. 2007

The Journal of Immunology

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Cells may discriminate among ligands with different dwell times for receptor binding through a mechanism called kinetic proofreading in which the formation of an activated receptor complex requires a progression of events that is aborted if the ligand dissociates before completion. This mechanism explains how, at equivalent levels of receptor occupancy, a rapidly dissociating ligand can be less effective than a more slowly dissociating analog at generating distal cellular responses. Simple mathematical models predict that kinetic proofreading is limited to the initial complex; once the signal passes to second messengers, the dwell time no longer regulates the signal. This suggests that an assay for kinetic proofreading might be used to determine which activation events occur within the initial signaling complex. In signaling through the high affinity IgE receptor FcεRI, the transmembrane adaptor called linker for activation of T cells (LAT) is thought to nucleate a distinct secondary complex. Experiments in which the concentrations of two ligands with different dwell times are adjusted to equalize the level of LAT phosphorylation in rat basophilic leukemia 2H3 cells show that Erk2 phosphorylation, intracellular Ca2+, and degranulation exhibit kinetic proofreading downstream of LAT phosphorylation. These results suggest that ligand-bound FcεRI and LAT form a complex that is required for effective signal transmission.

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Synthesis and Secretion of Monocyte Chemotactic Protein-1 Stimulated by the High Affinity Receptor for IgE

Cited by 16 publications

References 27 publications

The novel flavone tetramethoxyluteolin is a potent inhibitor of human mast cells

The novel flavone tetramethoxyluteolin is a potent inhibitor of human mast cells

Piper nigrum Fruit Extract Prevents TMA-Induced Allergic Contact Dermatitis by Regulating Th2 Cytokine Production

Kinetic Proofreading of Ligand-FcεRI Interactions May Persist beyond LAT Phosphorylation

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