Synthesis and spectroscopic characterization of a target-specific nanohybrid for redox buffering in cellular milieu

Functionalized Two-Dimensional Carbon Nitride Nanodots Detect and Reverse Lead Toxicity in the Physiological Milieu

“…2′,7′-Dichlorofluorescein (DCFH), a very popular chemical for the quantification of ROS generation, was prepared from DCFH-DA via a de-esterification reaction at room temperature following a standardized protocol described in earlier studies. , ROS generated in the reaction medium reacted with DCFH and converted it to DCF, which has a characteristic fluorescence emission maximum at 520 nm on excitation at 488 nm. DCFH was allowed to react with C-C 3 N 4 NDs in an aqueous medium, and the fluorescence was recorded using a Fluorolog Model LFI-3751 (Horiba-Jobin Yvon, Edison, NJ) spectrofluorimeter.…”

Cascade nanozymatic network mimicking cells with selective and linear perception of H2O2

“…Owing to their low cost, ease of production, and high stability against biodegradation and denaturation, 17–20 nanomaterials with enzyme-like activity (termed nanozymes) have drawn increasing attention in various fields, such as biosensors, immunoassays, and cancer therapeutics. 21–29 Along this line, nanozyme-based cascade systems have already been reported for signal amplification and selective biosensing. 30,31 However, to engineer more advanced behaviors, such as metabolic regulation and signal transduction, it is necessary to use a single stimulus to trigger diverse responses for a cascade system.…”

Chitosan functionalized Mn3O4 nanoparticles counteracts ulcerative colitis in mice through modulation of cellular redox state

“…One of the emerging solutions to this unique paradox about the pleiotropic role of ROS in disease progression is the introduction of redox-modulating nanomaterials with potential electron-donating as well as accepting capability to maintain precise balance between cellular oxidative eustress and distress (i.e., redox buffering; see ref. 23 for details). In recent years, some inorganic nanoparticles with anti-oxidant properties showed some promising results in the management of UC.…”