Synthesis and stability evaluation of size-controlled gold nanoparticles via nonionic fluorosurfactant-assisted hydrogen peroxide reduction

Li, Qianqian; Lu, Biqi; Zhang, Lijuan; Lu, Chao

doi:10.1039/c2jm31528a

Cited by 38 publications

(15 citation statements)

References 48 publications

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“…These authors describe a preliminary mechanism in which the reduction of Au(III), Ag (II), Pt(IV), or Zn(II) leading to the formation of AuNCs, AgNCs, ZnNCs, and PtNCs in situ could be attributed to the high levels of either the reactive oxygen and nitrogen species (ROS and RNS) present in tumorigenic cells, or the elevated levels of intracellular reductants such as GSH-GSSG and NAD(P)H that work to mitigate ROS and RNS in tumorigenic cells (2,7,8,19,20). In fact, H 2 O 2 has been demonstrated as a reducing agent for gold nanoparticle synthesis (21)(22)(23)(24)(25), as has glutamate, a building block of GSH (26)(27)(28). This implied that only cells which are tumorigenic in nature should be capable of synthesizing P:NCs in situ.…”

Section: Introductionmentioning

confidence: 98%

In situSynthesis of Fluorescent Gold Nanoclusters by Nontumorigenic Microglial Cells

West¹,

Schaeublin

Griep³

et al. 2016

ACS Appl. Mater. Interfaces

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To date, the directed in situ synthesis of fluorescent gold nanoclusters (AuNCs) has only been demonstrated in cancerous cells, with the theorized synthesis mechanism prohibiting AuNC formation in nontumorigenic cell lines. This limitation hinders potential biostabilized AuNC-based technology in healthy cells involving both chemical and mechanical analysis, such as the direct sensing of protein function and the elucidation of local mechanical environments. Thus, new synthesis strategies are required to expand the application space of AuNCs beyond cancer-focused cellular studies. In this contribution, we have developed the methodology and demonstrated the direct in situ synthesis of AuNCs in the nontumorigenic neuronal microglial line, C8B4. The as-synthesized AuNCs form in situ and are stabilized by cellular proteins. The clusters exhibit bright green fluorescence and demonstrate low (<10%) toxicity. Interestingly, elevated ROS levels were not required for the in situ formation of AuNCs, although intracellular reductants such as glutamate were required for the synthesis of AuNCs in C8B4 cells. To our knowledge, this is the first-ever demonstration of AuNC synthesis in nontumorigenic cells and, as such, it considerably expands the application space of biostabilized fluorescent AuNCs.

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Section: Introductionmentioning

confidence: 98%

In situSynthesis of Fluorescent Gold Nanoclusters by Nontumorigenic Microglial Cells

West¹,

Schaeublin

Griep³

et al. 2016

ACS Appl. Mater. Interfaces

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“…Aer the pioneering work of El-Sayed and co-workers for the synthesis of cubic and tetrahedral platinum nanoparticles, 8 many efforts have been devoted to prepare metallic nanoparticles with different shapes such as cube, tetrahedron, octahedron, cuboctahedron, decahedron, and icosahedron, which are usually enclosed by low-index facets of {100} and {111}. [9][10][11][12][13][14][15][16] Recently, metallic nanoparticles with high-index facets have been successfully prepared by wet chemistry routes. 17,18 These nanoparticles exhibit much higher catalytic activity than those enclosed by low-index facets because of a large density of lowcoordinated atoms situated on steps, ledges and kinks.…”

Section: Introductionmentioning

confidence: 99%

Single-crystalline and multiple-twinned gold nanoparticles: an atomistic perspective on structural and thermal stabilities

Huang

Shao

et al. 2014

RSC Adv.

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Morphologies of gold nanoparticles play an important role in determining their chemical and physical (catalytic, electronic, optical, etc.) properties. Therefore, a fundamental understanding of the morphological stability is of crucial importance to their applications. In this article, we employed atomistic simulations to systematically investigate the structural and thermal stabilities of gold particles with eight representative nanoshapes, including single-crystalline and multiple-twinned structures. Our investigation has revealed that the truncated octahedron and the octahedron possessed the best structural stability, while the tetrahedron and the icosahedron did the worst. Further analyses have discovered different thermal stabilities and diverse melting behaviors in these particles. Especially, an inhomogeneous melting of the icosahedron was disclosed, and the relevant mechanism was elucidated.This study provides significant insight not only into the experimental preparation of gold nanoparticles but also into the design of gold nanostructures with both high catalytic activity and excellent stability.

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“…Since there have been reports in the literature about methods in which hydrogen peroxide is used as a reducing agent for AuNPs [17,18], it was considered necessary to demonstrate the stabilizing role of collagen, critical for the formation of our described nanoparticles. Therefore, as a control measurement, we reproduced the exact synthesis steps mentioned in the Methods section but without the addition of collagen.…”

Section: Resultsmentioning

confidence: 99%

Anisotropic Gold Nanoparticle-Cell Interactions Mediated by Collagen

Marişca

Leopold

2019

Materials

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Gold nanoparticles (AuNPs) are the groundwork of a large variety of applications in the biomedical field. Further development and a better understanding of this versatile platform will lead to an expansion of potential applications. In this study, we propose a facile synthesis of AuNPs using hydrogen peroxide as a reducing agent and collagen as a stabilizing agent. Our synthetic approach results in “raspberry”-like AuNPs with a mean diameter of 60 nm, as revealed by electron microscopy. The optical properties of the AuNPs were assessed by UV-Vis and surface-enhanced Raman scattering (SERS), and their stability and in vitro cytotoxicity were evaluated as well. HeLa cell viability values were only modestly affected compared to control, with the highest concentration tested displaying a 20% decrease in cellular viability. The dose-dependent cellular internalization in the 20–60 nM range indicate the highest internalization rate at 60 nM and uptake values as high as 35%. This result correlated well with the viability results. These type of anisotropic AuNPs are proposed for biomedical applications such as hyperthermia, contrast agents or imaging. Therefore, our findings offer a platform for potential biological applications such as sensing and imaging, due to their unique physico-chemical features.

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Synthesis and stability evaluation of size-controlled gold nanoparticles via nonionic fluorosurfactant-assisted hydrogen peroxide reduction

Cited by 38 publications

References 48 publications

In situSynthesis of Fluorescent Gold Nanoclusters by Nontumorigenic Microglial Cells

In situSynthesis of Fluorescent Gold Nanoclusters by Nontumorigenic Microglial Cells

Single-crystalline and multiple-twinned gold nanoparticles: an atomistic perspective on structural and thermal stabilities

Anisotropic Gold Nanoparticle-Cell Interactions Mediated by Collagen

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