Synthesis and Stability of Resveratrol-Conjugated Gold Nanoparticles Modified With Polyethylene Glycol

Abstract

Objective: Gold nanoparticles (AuNPs) are highly useful for drug delivery, but their application is limited by their stability as they readily aggregate.This issue can be prevented by adding a stabilizing agent such as resveratrol (RSV), which is a polyphenol derived from plants, that is used to preventcancer. Therefore, we propose a novel method to prepare stable RSV-conjugated nanoparticles modified with polyethylene glycol (RSV-AuNP-PEG).Methods: In the first step, the Turkevich method was used to synthesize the AuNPs. Then, PEG was added as stabilizer agent and conjugated with RSV.The synthesized conjugates were characterized using ultraviolet-visible spectrophotometry, Fourier transform infrared spectroscopy, particle sizeanalysis, and high-performance liquid chromatography.Results: The obtained RSV-AuNP-PEG had a particle size of 83.93 nm with a polydispersity index (PDI) of 0.562 and formed a translucent purple-redfluid in solution. The zeta potential was −22.9 mV, and the highest entrapment efficiency was 75.86±0.66%. For comparison, the RSV-AuNP solutionwas purple and turbid, the particle size was 51.97 nm with a PDI of 0.694, and the zeta potential was −24.6 mV. The stability test results showed thatthe storage stability of RSV-AuNP-PEG was better than that of AuNP-RSV. Further, the RSV-AuNP-PEG was shown to be most stable in 2% bovine serumalbumin (BSA) while the AuNP-RSV was most stable in 2% BSA in phosphate-buffered saline pH 7.4.Conclusion: These results show that modification of RSV-conjugated AuNPs with PEG effectively prevents their aggregation in storage, but only incertain mediums.

Developing Gold-Resveratrol Nanoconjugates for Management of Rheumatoid Arthritis (RA)

“…Prior observations by Park et al (21) indicated the appearance of absorbance at 547 nm and successful synthesis of Res-AuNPs. Similarly, Iswandana et al (17) observed ultraviolet-visible spectra of gold nanoparticles with a peak at 528.50 nm and AuNPs-PEG-Res absorbed light at 529 nm. The chemical surfaces and functional group endpoints of AuNPs and AuNPs-PEG-Res were characterized using FT-IR spectroscopy.…”

“…The only difference observed in the FTIR of AuNPs-PEG-Res over Res is the shift of a broad band observed between 3259.70 cm −1 , 2113.99 cm −1 and 1635.64 cm −1 . The appearance of a peak observed at 3749.64 cm −1 is related to the PEG-(N-H) (17) . The strong diffraction peaks with 2θ values at 38.2º, 44.4º, 64.7º, and 77.7º are associated with the Bragg's reflections of 111, 200, 220, and 311 planes related to the face-centered cubic (FCC) of AuNPs.…”

“…2.175 ml of 0.04 mM aqueous PEG was added while stirring. To obtain Res-conjugated AuNPs-PEG, 3.425 mL of aqueous Res (made by dissolving 0.5 mg of Res powder in 15 mL of D.W) was dropped into the mixture dropwise, and stirring at 150 rpm continued for 2 hours at 40°C (AuNPs-PEG-Res) (17) .…”