Synthesis and study of branched hyaluronic acid with potential anticancer activity

Buffa, Radovan; Nešporová, Kristina; Basarabová, Ivana; Halamková, Pavlína; Svozil, Vít; Velebný, Vladimı́r

doi:10.1016/j.carbpol.2019.115047

Cited by 13 publications

(4 citation statements)

References 18 publications

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“…On the following day, the cells were treated with 200 μL of a dilution series of extracts from the dressings (100%, 50%, 20%, or 10% of the original extract diluted with culture medium supplemented with 10% FBS) for 24, 48, and 72 h. Control cells were treated with a standard 10% FBS culture medium. Viability was measured as described previously 54 using the 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) assay. MTT stock solution (20 μL; c = 5 mg/ mL) was added to the cell culture medium in each well.…”

Section: Gene Expressionmentioning

confidence: 99%

Effects of wound dressings containing silver on skin and immune cells

Nešporová

Pavlík

Šafránková

et al. 2020

Sci Rep

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Wound dressings with silver have been shown to be cytotoxic in vitro. However, the extrapolation of this cytotoxicity to clinical settings is unclear. We applied dressings with various forms of silver on porcine skin ex vivo and investigated silver penetration and DNA damage. We assessed antimicrobial efficacy, cytotoxicity to skin cells, and immune response induced by the dressings. All dressings elevated the DNA damage marker γ-H2AX and the expression of stress-related genes in explanted skin relative to control. This corresponded with the amount of silver in the skin. The dressings reduced viability, induced oxidative stress and DNA damage in skin cells, and induced the production of pro-inflammatory IL-6 by monocytes. The oxidative burst and viability of activated neutrophils decreased. The amount of silver released into the culture medium varied among the dressings and correlated with in vitro toxicity. However, antimicrobial efficiencies did not correlate strongly with the amount of silver released from the dressings. Antimicrobial efficiency and toxicity are driven by the form of silver and the construction of dressings and not only by the silver concentration. The damaging effects of silver dressings in ex vivo skin highlight the importance of thorough in vivo investigation of silver dressing toxicity.

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Section: Gene Expressionmentioning

confidence: 99%

Effects of wound dressings containing silver on skin and immune cells

Nešporová

Pavlík

Šafránková

et al. 2020

Sci Rep

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“…[4,25,26] Oligosaccharides of HA are important in tumor growth, fibroblast proliferation, and angiogenesis. [27] It is worth noting that there is no clear division of M W range at present and the corresponding cell biology is not entirely clear as well. The classification here is only one of the classification methods.…”

Section: Influence Of M Wmentioning

confidence: 99%

Tailoring Hyaluronic Acid Hydrogels for Biomedical Applications

Luo,

Wang,

et al. 2023

Adv Funct Materials

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Hyaluronic acid (HA) is an attractive anionic polysaccharide polymer with inherent pharmacological properties and versatile chemical groups for modification. Due to their water retention ability, biocompatibility, biodegradation, cluster of differentiation‐44 targeting, and highly designable capacity, HA hydrogels have been an emerging biomaterial, showing tailoring performance in terms of chemical modifications and hydrogel forms. Various preparation technologies have been developed for the fabrication of the tailoring HA hydrogels with unique structures and functions. They have been utilized in diverse biomedical applications like drug delivery and tissue engineering scaffolds. Herein, this review comprehensively summarizes the HA derivatives with different molecule weights and functional modifications. Then the various fabrication methods to obtain tailoring hydrogels in the forms of nanogel, nanofiber, microparticle, microneedle patch, injectable hydrogel, and scaffold are reviewed as well. The emphasis is focused on the shining biomedical applications of these tailoring HA hydrogels in anti‐bacteria, anti‐inflammation, wound healing, cancer treatment, regenerative medicine, psoriasis treatment, diagnosis, etc. The potentials and prospects are subsequently given to inspire further investigation, aiming at accelerating product translation from research to clinic.

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“…Intermediate molecular weight (iM W ) (1 × 10 4 to 1 × 10 6 Da) forms are used to inject cross-linked products and in chronic wound healing. HA oligosaccharides (lM W ≤1 × 10 4 Da) are related to numerous activities, including fibroblast proliferation, blood vessel structure, inflammatory processes and tumour growth, among others [107][108][109][110]. It has been suggested that iM W and lM W HA can penetrate the epidermis after topical application, with iM W functioning as an inductor of cellular proliferation [111,112].…”

Section: Derivativesmentioning

confidence: 99%

Trends in biopolymer science applied to cosmetics

Mendoza‐Muñoz

Leyva-Gómez

Piñón-Segundo

et al. 2023

Intern J of Cosmetic Sci

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The term biopolymer refers to materials obtained by chemically modifying natural biological substances or producing them through biotechnological processes. They are biodegradable, biocompatible and non‐toxic. Due to these advantages, biopolymers have wide applications in conventional cosmetics and new trends and have emerged as essential ingredients that function as rheological modifiers, emulsifiers, film‐formers, moisturizers, hydrators, antimicrobials and, more recently, materials with metabolic activity on skin. Developing approaches that exploit these features is a challenge for formulating skin, hair and oral care products and dermatological formulations. This article presents an overview of the use of the principal biopolymers used in cosmetic formulations and describes their sources, recently derived structures, novel applications and safety aspects of the use of these molecules.

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Synthesis and study of branched hyaluronic acid with potential anticancer activity

Cited by 13 publications

References 18 publications

Effects of wound dressings containing silver on skin and immune cells

Effects of wound dressings containing silver on skin and immune cells

Tailoring Hyaluronic Acid Hydrogels for Biomedical Applications

Trends in biopolymer science applied to cosmetics

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