Synthesis and Study of Structural Properties of Calcium Oxide Nanoparticles Produced by Laser-Induced Plasma and its Effect on Antibacterial Activity

Abstract

A LIBS technique was used to investigate the antibacterial activity of calcium oxide nanoparticles (CaO NPs). CaO NPs were prepared using a Q-switched Nd: YAG pulsed laser with a fundamental wavelength of 1064 nm at different energies (400-600 mJ) and constant frequency (6 Hz). A calcium powder sample was prepared after being pressed into a disc with a diameter of 1 cm. Analyzing X-ray diffraction (XRD) showed the crystalline structure of CaO NPs, crystalline size was 30.99±2 and 34.20±2.2 nm for laser energy 500 and 600 mJ. (FE-SEM) to reveal the topography of produced CaO NPs, the results showed a homogenous compact and dense surface with the formation of CaO NPs like flakes, cubes, and tubes. Atomic force microscopy (AFM) has shown that the CaO NPs were nanoscale and had a coordinated surface structures. The results also revealed the stabilizing zeta potential of the prepared CaO NPs, average (ZP) -18.3±1.6 mV in 600 mJ and -8.8±2.3 mV for 500 mJ. Different laser energies used in preparing CaO NPs resulted in the varying killing of the number of bacteria Klebsiella pneumoniae and Staphylococcus aureus bacteria. Complete bacterial inhibition or cell growth inactivation was found when the laser energy prepared for the CaO NPs was 600 mJ.

Spectroscopic and antibacterial activities of cobalt and nickel nanoparticles: a comparative analysis

“…9). Similar antibacterial activities have been reported by several researchers for NPs (Abbas and Aadim 2022;Ahghari et al 2020;Bensy et al 2022). Ahghari et al (Ahghari et al 2020) reported antibacterial activities of NiNPs against Staphylococcus aureus and Escherichia coli.…”

“…Several metal oxide nanoparticles have been reported for their potential antibacterial activities against Gramnegative and Gram-positive bacteria, consisting of Klebsiella pneumoniae, Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus. These metal oxide nanoparticles include Al 2 O 3 (Jawad et al 2021;Karimi et al 2021), CuO, Cu 2 O, ZnO (Asamoah et al 2020;Karuppannan et al 2021;Han et al 2022;Khosravi et al 2022), CaO (Abbas and Aadim 2022), MnO 2 , MgO (Ogunyemi et al 2020;Amrulloh et al 2021), TiO 2 (Wang et al 2020;Zhang et al 2021), Fe 3 O 4 (Azizabadi et al 2021;Kamali et al 2022), Ag 2 O (Rashidi Ranjbar et al 2020;Dharmaraj et al 2021;Mahlambi and Moloto 2022), NiO (Kannan et al 2020;Christy et al 2021), and SiO 2 (Attallah et al 2022;Tran et al 2023). The antibacterial activity lies in its physical and chemical properties, which include morphology, chemical composition, particle size, surface characteristics, solubility, crystallinity, aggregation, and crystal phase.…”

Influence of Laser Energy on the Structural and Optical Properties of Sn Nanoparticles produced with Laser-Induced Plasma

“…The crystal structure, orientation, and grain size of a material may be identified by the use of XRD [19], which is a potent, non-destructive approach to material characterization. Scherer's formula was used to determine how large the individual crystals are (D) in a polycrystalline film [9,20]:…”

“…This focused laser beam induces excitation and ionization of the target material, leading to the formation of a plasma plume [8]. This plume consists of a mixture of electrons, neutral atoms, and ions, which are rich in energy [9]. Subsequently, the temperature of the region involved in the process experiences rapid and significant elevation, reaching the threshold required for vaporization [10].…”