Synthesis, Antifungal Activity, and Cytotoxicity of AgBr-NP@CTMAB Hybrid and Its Application in PMMA

Abstract

Objective To synthesize and determine the antifungal activity of AgBr-nanoparticles (NP) @CTMAB (cetyltrimethyl-ammonium bromide) against Candida albicans ( C. albicans ) for use in the field of denture cleaning. Methods The morphology and structure of AgBr-NP@CTMAB were characterized by IR, UV-Vis, XRD and SEM. The antifungal potential of AgBr-NP@CTMAB against C. albicans was determined by colony formation assay and growth curve analysis. PMMA containing AgBr-NP@CTMAB was prepared, and the long-term antifungal efficacy was analyzed. The effect against C. albicans biofilm was analyzed by SEM and OD600 , and the color changes of the specimens were observed by stereomicroscopy after 1 week of incubation. Cytotoxicity to human oral gingival fibroblasts and oral mucosal epithelial cells was detected by Cell Counting Kit-8 (CCK-8) in vitro. Results The compound showed a good crystalline phase, the presence of AgBr nanoparticles and the hybridization of CTMAB+ with AgBr-NPs. AgBr-NP@CTMAB showed significant antifungal activity against C. albicans at concentrations of 10 μg/mL and 20 μg/mL. PMMA specimens containing AgBr-NP@CTMAB showed no long-term antifungal effect against C. albicans biofilm. The clearance rate of C. albicans attached to PMMA was 44.73% after soaking in 10 µg/mL AgBr-NP@CTMAB solution for 30 min and 91.35% for 8 h. There was no significant residual cytotoxicity or visual color change after soaking. Significance AgBr-NP@CTMAB showed promising potential treatment for denture cleaners.

The Antibacterial Activity of Kaempferol Combined with Colistin against Colistin-Resistant Gram-Negative Bacteria

“…6 ) were added to the medium and incubated for 12 h. Wells without drugs were used as negative controls. After incubation, we changed the cell culture medium ( 25 – 32 ). After that, 10 μL of CCK-8 (Dojindo Laboratory, Japan) was added to each well, which was then left to culture at room temperature in the dark for 2 h. Absorbance was recorded at 450 nm with a microplate instrument.…”

Anti-Microbial Effect of AgBr-NP@CTMAB on Streptococcus Mutans and Assessment of Surface Roughness Hardness and Flexural Strength of PMMA

“…The synthesis and physical measurements of AgBr-NP@CTMAB Hybrid Material has been reported in our previous paper. 15 The macroscopical size of AgBr-NP@CTMAB can be measured as 20×80 μm according to the Scanning Electron Microscope (SEM) image as shown in the Figure 1A of this publication. Its microcosmic has been constructed as the encapsulation of AgBr nano-clusters (Ag4Br8 tetramer, about 1.6×0.44 nm) into the CTMAB cations.…”

“… 14 The solution of AgBr-NP@CTMAB shown similar antifungal ability to suppress biofilm development as Polident in a previous investigation by our lab, without noticeable residual cytotoxicity. 15 AgBr-NP@CTMAB has a lot of potential as a novel ingredient in denture cleaner since its single ingredient can achieve the same efficacy as Polident, which has four active components. Despite the well-established understanding that S. mutans, a prominent contributor to dental caries, may lead to systemic illnesses such as subacute bacterial endocarditis upon entering the circulation, the specific impact of AgBr-NP@CTMAB’s antimicrobial activity on the growth of S. mutans remains unknown.…”

Biomineralization-Inspired Anti-Caries Strategy Based on Multifunctional Nanogels as Mineral Feedstock Carriers

“…Cell viability was determined using a cell counting kit-8 (CCK-8; Dojindo Laboratories, Japan). 26 In brief, human gingival epithelial cells (hGECs) cultured in medium (10% foetal bovine serum, 90% α-MEM and 1% penicillin/ streptomycin) were incubated in a 96-well microplate for 24 h (3000 cells per well). Then, solutions of nanogels at different concentrations (0-100 mg/mL) were added for further incubation for 1 day, 3 days, and 5 days.…”