Synthesis, Application, and Tracking of Magnetic Carbon-Coated Nanoparticles in Plants

Abstract

The behavior of nanoparticles inside plants is gaining importance for its implications in research about putative applications and toxicology. Magnetic carbon-coated nanoparticles can be easily traced through plant tissues using simple and affordable histological techniques. Here we present a methodology for the synthesis of such nanoparticles. We also describe methods for growing plants using rhizotrons (in order to observe the roots), procedures for applying the nanoparticles, taking and processing the samples.

Evaluation of the effects of polymeric chitosan/tripolyphosphate and solid lipid nanoparticles on germination of Zea mays, Brassica rapa and Pisum sativum

“…Various studies have shown that nanoparticles can penetrate the cell walls in plant roots, becoming internalized in the cells and/or reaching the vascular system of the plant and being distributed to other plant organs such as the stem, leaves, and fruit (Pérez- de-Luque et al, 2012;Wang et al, 2012). Chen et al (2010) investigated the effects of carbon nanotubes on A. cepa and found that larger and more hydrophobic particles could create barriers in the pores of the cell walls, affecting the physiological state of the plant and leading to cell damage.…”

“…Studies have been conducted to obtain a better understanding of the biological effects of nanomaterials, as well as the mechanisms of their uptake and translocation in plants (Pérez- de-Luque et al, 2012). Physico-chemical characteristics of nanoparticles such as size, zeta potential, and concentration determine their interactions with plant cells and can influence the physiological responses (Thuesombat et al, 2014).…”

Desenvolvimento e avaliação de sistemas nanocarreadores para o regulador de crescimento vegetal ácido giberélico

“…Various studies have shown that nanoparticles can penetrate the cell walls in plant roots, becoming internalized in the cells and/or reaching the vascular system of the plant and being distributed to other plant organs such as the stem, leaves, and fruit (PÉREZ- DE-LUQUE et al, 2012;WANG, et al, 2012). Chen e al., (2010) investigated the effects of carbon nanotubes on A. cepa and found that larger and more hydrophobic particles could create barriers in the pores of the cell walls, affecting the physiological state of the plant and leading to cell damage.…”

“…Studies have been conducted to obtain a better understanding of the biological effects of nanomaterials, as well as the mechanisms of their uptake and translocation in plants (PÉREZ-DE-LUQUE et al, 2012). Physico-chemical characteristics of nanoparticles such as size, zeta potential, and concentration determine their interactions with plant cells and can influence the physiological responses (THUESOMBAT et al, 2014).…”