Synthesis, characterization and antibacterial activity of thymol-loaded polylactic acid microparticles entrapped with essential oils of varying viscosity

Biswal, Agni Kumar; Vashisht, Isha; Khan, Aamir Ayaz; Sharma, Shivangi; Saha, Sampa

doi:10.1007/s10853-019-03593-7

Cited by 20 publications

(17 citation statements)

References 52 publications

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“…As expected, the MICs recorded for the PLA/AE film in present study were far higher than the reported MICs for pure AE in the aforementioned study. The similar observation was made by Biswal et al [20], who demonstrated a considerable antibacterial activity of thymol released from PLA-based micro-particles. The antibacterial activity of the fabricated PLA films against the tested foodborne pathogens evaluated by disc diffusion agar is shown in Table 4.…”

Section: Antibacterial Properties Of Filmssupporting

confidence: 83%

“…For the evaluation of the antibacterial properties of the films, firstly, the minimum inhibitory concentration (MIC) of the developed composites was assessed against the abovementioned pathogens following the procedure detailed by Biswal et al [20], and then the standard agar disc diffusion method was applied according to the method described in the literature [21].…”

Section: Antibacterial Properties Of Filmsmentioning

confidence: 99%

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Development of Antibacterial Biocomposites Based on Poly(lactic acid) with Spice Essential Oil (Pimpinella anisum) for Food Applications

et al. 2021

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Among the main biodegradable food packaging materials, poly-lactic acid (PLA) is a commercially successful polymer used notably in the food packaging industry. In this study, active PLA films containing different percentage of anise essential oil (AE) (0, 0.5, 1 and 1.5% v/v) were developed, and characterized by physical, mechanical and antibacterial analysis. Based on physical examinations, thermal stability of PLA/AE films was greater than the neat PLA film, and the minimum water vapor permeability (WVP) was recorded for PLA/0.5AE film (1.29 × 10 11 g/m s), while maximum WVP was observed for PLA/1.5AE (2.09 × 1011 g/m s). Moreover, the lightness and yellowness of the composites were decreased by the addition of AE. For the PLA composites with 1.5% AE, the tensile strength decreased by 35% and the elongation break increased by 28.09%, comparing to the pure PLA. According to the antibacterial analysis, the minimum inhibitory concentrations of PLA/AE film were 5 to 100 mg/mL and the active composite could create visible inhibition zones of 14.2 to 19.2 mm. Furthermore, the films containing AE inhibited L. monocytogenes and V. parahaemolyticus in a concentration-dependent manner. The confirmation of the success of the incorporation of EOs into the PLA films was further evaluated using principal component analysis, where positive results were obtained. In this context, our findings suggest the significant potency of AE to be used as an antibacterial agent in active food packaging.

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Section: Antibacterial Properties Of Filmssupporting

confidence: 83%

Section: Antibacterial Properties Of Filmsmentioning

confidence: 99%

Development of Antibacterial Biocomposites Based on Poly(lactic acid) with Spice Essential Oil (Pimpinella anisum) for Food Applications

et al. 2021

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“…The death of bacteria caused by bactericidal agents tethered onto the surface happens either due to the disruption of bacterial membrane or via a specific interaction of immobilized antibacterial agent with a target biomolecule present onto the surface of the bacteria . Bactericidal agents can either be released out from the surface, which are biocide leaching coatings, or they are immobilized onto the surface and act as contact-killing coatings. ,− For contact-killing coatings the death of bacteria is generally due to the disruption of bacterial membrane caused by the interactions taking place between the bacterial membrane and the bactericidal agent. The bacteriostatic agents relating to the antiadhesive coatings present on the biomaterial surfaces tend to decrease the conformational entropy of the polymeric chains by tremendous increase in the osmotic pressure and repel the bacteria. , In the case of antibiotics such as fusidic acid, gentamycin, chlorhexidin, and many others included in polymeric coatings applied on catheters showed very low bacterial adhesion onto the implant’s surface.…”

Section: Working Mechanism Of Polymeric Brushes As Infection-resistan...mentioning

confidence: 99%

Infection Resistant Surface Coatings by Polymer Brushes: Strategies to Construct and Applications

Dhingra

Sharma

Saha

2022

ACS Appl. Bio Mater.

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Bacteria-assisted infections on biomaterials used inside a body as an implant/device are one of the major threats to human health. Microbial-resistant coatings on biomaterials can potentially be considered to mitigate the biomaterial-associated infections. Usually biomaterials with leachable antimicrobial coatings, though economically attractive, provide only short-term protection of the surface against bacteria. Therefore, a stable, nonfouling or bactericidal, and biocompatible polymeric coating is highly desirable. In this regard, polymer brushes, defined as polymer chains tethered to a surface by one end, with suitable antiinfective functionality, represent a useful class of stable coatings which are covalently connected to the underlying surface, thus prolonging the infection resistance of the coated surface. Surface-initiated atom transfer radical polymerization (SI-ATRP) is a versatile technique for the generation of polymeric brushes via "grafting from" way. In this review, we have attempted to give a brief overview about the recent developments of surface coatings by infection-resistant polymer brushes synthesized via SI-ATRP and their applications in the biomedical field. On the basis of their charges, these anti-infective brushes can be classified into five different categories such as neutral, cationic, anionic, zwitterionic, and mixed brushes. The working mechanism of each type of brush in repelling (nonfouling/bacteriostatic) and/or killing (bactericidal) the bacteria has also been discussed. A brief summary of their future scope is also highlighted.

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“…This may drag the Ac-dextran core toward the interface of PLGA and PVA. Moreover, due to the slow precipitation rate of low-viscous PLGA as a consequence of the low diffusion rate of chloroform from the PLGA phase, it may not get sufficient time to engulf low-viscous Ac-dextran, which tends to migrate to the outer water phase, thus dislocating the center (Figure b). , In the case of S1, the formation of Ac-dextran/PLGA double-layered (shell/core) microparticles was not thermodynamically favorable, as predicted by the spreading coefficient theory (Table , S M < 0). Therefore, Ac-dextran with double mass may kinetically trap low-viscous PLGA because of the high mass ratio of Ac-dextran/PLGA (2:1), thus predominating kinetics over thermodynamics.…”

Section: Resultsmentioning

confidence: 96%

pH-Sensitive Acetalated Dextran/PLGA-Based Double-Layered Microparticles and Their Application in Food Preservation

Biswal

Thodikayil

Saha

2021

ACS Appl. Bio Mater.

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Double-layered microparticles (150–190 μm) composed of biodegradable poly(lactic-co-glycolic acid) (PLGA) and pH-responsive acetalated dextran were fabricated using a one-step emulsion solvent evaporation technique. Nearly 80% dextran was released from the microparticles after 20 days of incubation in pH ∼ 5 medium, and the complete disappearance of shell (Ac-dextran) layer was also evident from scanning electron microscopy (SEM) images after 20 days under the same condition. However, the Ac-dextran shell was found to remain unchanged in neutral pH. Dual actives such as antibacterial (benzoic acid) and antioxidant (tocopherol) were incorporated in the shell and core of the microparticles to exploit their applications as food-preserving materials. An accelerated release of antibacterial and a controlled release of antioxidant were found to be useful for prolonging the shelf life of a low-pH food such as pork broth (pH ∼ 5) over 20 days by providing complete bacterial growth inhibition and high radical scavenging efficiency (70–90%).

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Synthesis, characterization and antibacterial activity of thymol-loaded polylactic acid microparticles entrapped with essential oils of varying viscosity

Cited by 20 publications

References 52 publications

Development of Antibacterial Biocomposites Based on Poly(lactic acid) with Spice Essential Oil (Pimpinella anisum) for Food Applications

Development of Antibacterial Biocomposites Based on Poly(lactic acid) with Spice Essential Oil (Pimpinella anisum) for Food Applications

Infection Resistant Surface Coatings by Polymer Brushes: Strategies to Construct and Applications

pH-Sensitive Acetalated Dextran/PLGA-Based Double-Layered Microparticles and Their Application in Food Preservation

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