Synthesis, characterization, and antiparasitic effects of zinc oxide nanoparticles-eugenol nanosuspension against Toxoplasma gondii infection

Cheraghipour, Kourosh; Khudair Khalaf, Amal; Moradpour, Kobra; Zivdari, Masoomeh; Beiranvand, Marjan; Shakib, Pegah; Mahmoudvand, Hossein; Marzban, Abdolrazagh

doi:10.1016/j.heliyon.2023.e19295

Cited by 9 publications

(3 citation statements)

References 53 publications

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“…We found that Lin‐ZNP significantly reduced the viability of tachyzoites with the SI > 10 indicating high specificity to parasites with low cytotoxicity to normal cells. Recent study documented that ZNP‐eugenol exhibited a notable decrease in the survival rate of intracellular parasites and tachyzoites of the Rh strain of T. gondii , demonstrating an IC 50 value of 30 μg/mL [31]. Another study demonstrated that ZNP displayed notable antileishmanial properties against Leishmania major promastigotes, achieving an IC 50 value of 37.8 μg/mL.…”

Section: Discussionmentioning

confidence: 99%

Linalool‐zinc oxide nanocomposite controls Toxoplasma gondii infection through inhibiting inflammation, oxidative stress, and pathogenicity

Baghdadi,

Albalawi,

Shater

et al. 2024

J Basic Microbiol

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The present in vitro and in vivo study aimed to fabricate and characterize linalool‐zinc oxide nanoparticles (Lin‐ZNP) and evaluate their effectiveness against Toxoplasma gondii infection in terms of inflammation, oxidative stress, and pathogenicity. Lin‐ZNP was synthesized using an ethanolic solution of polyvinyl alcohol. The anti‐Toxoplasma and cytotoxicity activities of Lin‐ZNP were investigated, along with its effects on nitric oxide (NO) production, caspase‐3 activity, and pro‐inflammatory genes. After treating T. gondii‐infected mice with Lin‐ZNP for 14 days, the number and size of tissue cysts, antioxidant potential, pro‐inflammatory cytokines, and T. gondii pathogenicity‐related genes were evaluated by real‐time polymerase chain reaction and Western blot analysis. The Lin‐ZNP composite showed a reduced tendency with an average size of 105 nm. Lin‐ZNP significantly reduced the viability of tachyzoites. The obtained selectivity index higher than 10, indicating high specificity for parasites with low cytotoxicity to normal cells. The Lin‐ZNP significantly (p < 0.05) increased the production of NO, caspase‐3 activity, and the expression levels of pro‐inflammatory genes. Lin‐ZNP significantly (p < 0.001) decreased the size and number of tissue cysts and caused a significant reduction in the level of malondialdehyde and a considerable increase (p < 0.001) in antioxidant enzymes and their expression genes. Lin‐ZNP significantly downregulated both mRNA and protein expression of the inflammation‐related markers associated with the TLRs/NF‐κB pathway. The expression levels of the T. gondii pathogenicity‐related genes were significantly downregulated (p < 0.05). The recent survey indicated that Lin‐ZNP manages T. gondii infection by its antioxidant activity and inhibiting the TLRs/NF‐κB pathway without toxicity in mice.

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Section: Discussionmentioning

confidence: 99%

Linalool‐zinc oxide nanocomposite controls Toxoplasma gondii infection through inhibiting inflammation, oxidative stress, and pathogenicity

Baghdadi,

Albalawi,

Shater

et al. 2024

J Basic Microbiol

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“…Silver nanoparticles (AgNPs) derived from plant extracts have demonstrated remarkable antiparasitic efficacy, displaying a reduced treatment duration and improved capacity to hinder parasite multiplication in comparison to conventional antiparasitic medications [ 159 ]. Numerous investigations suggest that silver nanoparticles prove advantageous in Photodynamic Therapy (PDT) owing to their exceptional stability, tunable size, optical characteristics, and ease of surface functionalization [ 160 ].…”

Section: Types Of Nanoparticlementioning

confidence: 99%

Advances in stabilization of metallic nanoparticle with biosurfactants- a review on current trends

2024

Heliyon

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“…The morphologies of the produced ZnO-NPs include spherical, nanocages, nanorings, nanocombs, nanobelts, nanohelices, nanoflowers, and nanowires, which may contribute to their biological effects (Król et al, 2017). In addition, it was found that green synthesized ZnO-NPs could be an effective alternative to antiparasitic drugs (Cheraghipour et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Green synthesis of zinc oxide/Allium sativum nano‐composite and its efficacy against murine cryptosporidiosis

Hamdy,

Ismail,

El‐Askary

et al. 2024

Microscopy Res & Technique

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Cryptosporidiosis is a global health problem threats life of immunocompromised patients. Allium sativum (A. sativum) is one of the therapeutic options for cryptosporidiosis. This study develops green synthesized ZnO‐NPs based on A. sativum extract, and assesses its therapeutic application in treating experimental cryptosporidiosis in immunosuppressed mice. FTIR, scanning electron microscopy, and zeta analyzer were used for characterization of bio ZnO‐NPs. The morphology of prepared materials appeared as sponge with many pores on the whole surface that allows the feasibility of bio ZnO‐NPs for different biological activities. Its structural analysis was highly stabilized with negative charge surface which indicated for well distribution into the parasite matrix. Twenty‐five immunosuppressed Cryptosporidium parvum infected mice, classified into 5 groups were sacrificed at 21th day after infection with evaluation of parasitological, histopathological, oxidative, and proinflammatory biomarkers. Treated mice groups with 50 and 100 mg/kg of AS/ZnO‐NPs showed a highly significant decline (79.9% and 83.23%, respectively) in the total number of expelled oocysts. Both doses revealed actual amelioration of the intestinal, hepatic, and pulmonary histopathological lesions. They also significantly produced an increase in GSH values and improved the changes in NO and MDA levels, and showed high anti‐inflammatory properties. This study is the first to report green synthesis of ZnO/A. sativum nano‐composite as an effective therapy in treating cryptosporidiosis which gave better results than using A. sativum alone. It provides an economical and environment‐friendly approach towards novel delivery synthesis for antiparasitic applications.Research Highlights Green synthesis of ZnO‐NPs was developed using A. sativum extract. The morphology of prepared ZnO‐NPs appeared as sponge with many pores on SEM The study evaluates its therapeutic efficacy against murine cryptosporidiosis The green synthesized ZnO‐NPs significantly reduced percent of oocyst shedding, improved the pathological changes, and showed high antioxidant and anti‐inflammatory potentials.

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Synthesis, characterization, and antiparasitic effects of zinc oxide nanoparticles-eugenol nanosuspension against Toxoplasma gondii infection

Cited by 9 publications

References 53 publications

Linalool‐zinc oxide nanocomposite controls Toxoplasma gondii infection through inhibiting inflammation, oxidative stress, and pathogenicity

Linalool‐zinc oxide nanocomposite controls Toxoplasma gondii infection through inhibiting inflammation, oxidative stress, and pathogenicity

Advances in stabilization of metallic nanoparticle with biosurfactants- a review on current trends

Green synthesis of zinc oxide/Allium sativum nano‐composite and its efficacy against murine cryptosporidiosis

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