Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA

balawi, Aisha Nawaf Al; Yusof, Nor Azah; Sijam, Kamaruzaman; Mohammad, Faruq; Wasoh, Helmi; Abbosh, Khulood Fahad Al; Al‐Lohedan, Hamad A.

doi:10.1155/2019/7064073

Cited by 5 publications

(5 citation statements)

References 32 publications

(35 reference statements)

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“…It is optimized to complete the HPAE polymerization reaction in hours, much faster than the days required for LPAE. Most importantly, compared with other publications, the synthesis conditions of HPAE are mild (do not require high temperature/low temperature, high pressure/vacuum, or even protection of inert conditions). Consequently, no large, specialized equipment is required to produce the coatings, making them easy to manufacture.…”

Section: Resultsmentioning

confidence: 98%

UV-Assisted Hyperbranched Poly(β-amino ester) Modification of a Silica Membrane for Two-Step Microfluidic DNA Extraction from Blood

Akshaya

Cong

et al. 2023

ACS Appl. Mater. Interfaces

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Integrating nucleic acid extraction in amplification-based point-of-care diagnostics will be a significant feature for next-generation point-of-care virus detection devices. However, extracting DNA efficiently on a microfluidic chip poses many technological and commercialization challenges, including manual steps, multiple instruments, pretreatment processes, and the use of organic solvents (ethanol, IPA) that inhibit detection, which is not viable with routine testing such as viral load monitoring of transplant patients for post-operative care. This paper presents a microfluidic system capable of two-step DNA extraction from blood using a UV-assisted hyperbranched poly(β-amino ester) (HPAE)-modified silica membrane for cytomegalovirus (CMV) detection in a rapid and instrument-free manner without the presence of amplification inhibitors. HPAEs of varying branch ratios were synthesized, screened, and coated on a silica membrane and bonded between two layers of poly(methyl methacrylate) (PMMA) substrates. Our system could selectively extract DNA from blood with an efficiency of 94% and a lower limit viral load of 300 IU/mL in 20 min. The extracted DNA was used as the template for real-time loop-mediated isothermal amplification (LAMP)-based detection of CMV and was found to produce a fluorescent signal intensity that was comparable with commercially extracted templates. This system can be integrated easily with a nucleic acid amplification system and used for routine rapid testing of viral load in patient blood samples.

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Section: Resultsmentioning

confidence: 98%

UV-Assisted Hyperbranched Poly(β-amino ester) Modification of a Silica Membrane for Two-Step Microfluidic DNA Extraction from Blood

Akshaya

Cong

et al. 2023

ACS Appl. Mater. Interfaces

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“…Additionally, 4,4′-cyclohexylidene bisphenol oxalates can be applied as a potential candidate in the SPE of DNA through a cost-effective and environmentally friendly approach. However, the extraction efficiency was low (arranged from 0.93% to 16.2%), and the eluted DNA contained impurities (the absorbance ratio of A260/A280 in all samples varied from 1.682 to 1.491, bellowed 1.8) [24].…”

Section: Polymer and Inorganic Nonmetallic Materialsmentioning

confidence: 94%

“…Hydrophobicity is an effective driving force for NA capture and can be derived from both structural and environmental hydrophobicity [22]. Ethyl ethanol was reported as an additive in a capture buffer to influence and enhance the capture of NAs to the polymer [24]. Dimethyl suberimidate was used as a novel nonchaotropic agent for amino-functionalized DE to capture NA, owing to the reversible crosslinking reaction between the amine groups and DNA fragments [12].…”

Section: Development Prospect Of Na Sorbentsmentioning

confidence: 99%

Solid‐phase extraction methods for nucleic acid separation. A review

Yue

et al. 2021

J of Separation Science

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The separation and purification of biomacromolecules such as nucleic acid is a perpetual topic in separation processes and bioengineering (fine chemicals, biopharmaceutical engineering, diagnostics, and biological characterization). In principle, the solid‐phase extraction for nucleic acid exhibits efficient phase separation, low pollution risk, and small sample demand, compared to the conventional liquid‐phase extraction. Herein, solid‐phase extraction methods are systematically reviewed to outline research progress and explore additional solid‐phase sorbents and devices for novel, flexible, and high‐efficiency nucleic acid separation processes. The functional materials capture nucleic acid, magnetic and magnetic‐free solid‐phase extraction methods, separation device design and optimization, and high‐throughput automatable applications based on high‐performance solid‐phase extraction are summarized. Finally, the current challenges and promising topics are discussed.

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“…This includes the use of many different adsorbent materials like glass particles [21], silica-based matrices [22,23], magnetic NPs [24], diatomaceous earth [25], anion-exchange materials [26], and cellulose matrices [27,28]; however, each material has its own advantages and disadvantages that are described briefly in Table 1. From the perspective that the effectiveness, equipment portability, and analysis costs have to be equally poised, the polymeric NCs are considered to serve as the suitable materials for the extraction of DNA and that too within many different kinds of polymer materials, the hybrid scaffolds made up of poly(bisphenol Z oxalate) are emerging as finest adsorbents for the selective and high efficiency DNA extraction [29].…”

Section: Introductionmentioning

confidence: 99%

“…By keeping in view of the selective and specific properties offered by poly(bisphenol Z oxalate) for the high efficiency DNA extraction and Si NP’s solid supported properties, the present study aims to develop an ideal NC system that has a very high DNA extraction efficiency. Our earlier study deal with the synthesis and characterization of pure poly(4,4′-cyclohexidene bisphenol oxalate) for the DNA extraction [29]. Further, to improve the DNA adsorption capabilities of the same poly(4,4′-cyclohexidene bisphenol oxalate) by means of influencing the inbuilt properties, the present study was carried where the polymer was used to form the composite with that of Si NPs.…”

Section: Introductionmentioning

confidence: 99%

DNA Adsorption Studies of Poly(4,4′-Cychlohexylidene Bisphenol Oxalate)/Silica Nanocomposites

et al. 2019

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The present study deals with the synthesis, characterization, and DNA extraction of poly(4,4′-cyclohexylidene bisphenol oxalate)/silica (Si) nanocomposites (NCs). The effects of varying the monomer/Si (3.7%, 7%, and 13%) ratio towards the size and morphology of the resulting NC and its DNA extraction capabilities have also been studied. For the NC synthesis, two different methods were followed, including the direct mixing of poly(4,4′-cyclohexylidene bisphenol oxalate) with fumed Si, and in situ polymerization of the 4,4′-cyclohexylidene bisphenol monomer in the presence of fumed silica (11 nm). The formed NCs were thoroughly investigated by using different techniques such as scanning electron microscopy (SEM), fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), powdered X-ray diffraction (XRD), and Brunauer–Emmett–Teller (BET) analysis where the results supported that there was the successful formation of poly(4,4′-cyclohexylidene bisphenol oxalate)/Si NC. Within the three different NC samples, the one with 13% Si was found to maintain a very high surface area of 12.237 m2/g, as compared to the other two samples consisting of 7% Si (3.362 m2/g) and 3.7% Si (1.788 m2/g). Further, the solid phase DNA extraction studies indicated that the efficiency is strongly influenced by the amount of polymer (0.2 g > 0.1 g > 0.02 g) and the type of binding buffer. Among the three binding buffers tested, the guanidine hydrochloride/EtOH buffer produced the most satisfactory results in terms of yield (1,348,000 ng) and extraction efficiency (3370 ng/mL) as compared to the other two buffers of NaCl (2 M) and phosphate buffered silane. Based on our results, it can be indicated that the developed poly(4,4′-cyclohexylidene bisphenol oxalate)/Si NC can serve as one of the suitable candidates for the extraction of DNA in high amounts as compared to other traditional solid phase approaches.

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Synthesis, Characterization, and Application of Poly(4,4'-Cyclohexylidene Bisphenol Oxalate) for Solid-Phase Extraction of DNA

Cited by 5 publications

References 32 publications

UV-Assisted Hyperbranched Poly(β-amino ester) Modification of a Silica Membrane for Two-Step Microfluidic DNA Extraction from Blood

UV-Assisted Hyperbranched Poly(β-amino ester) Modification of a Silica Membrane for Two-Step Microfluidic DNA Extraction from Blood

Solid‐phase extraction methods for nucleic acid separation. A review

DNA Adsorption Studies of Poly(4,4′-Cychlohexylidene Bisphenol Oxalate)/Silica Nanocomposites

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