Graphene Quantum Dots (GQDs) have attracted significant attention and applications in the field of biosensors, particularly for early detection of cervical cancer, due to their excellent electrochemical, optical, and biocompatibility properties. In this review, recent results on green methods for the synthesis of GQDs as well as their characterization techniques are discussed, with particular emphasis on structural, morphological, elemental, optical, and functional group analysis. In addition, a comprehensive overview of how the electrochemical and biocompatibility properties of GQDs enable their use in the development of sensing elements for biosensing applications is presented. This paper also highlights the most recent developments in the fabrication of GQDs-based biosensors for cervical cancer detection. Finally, the future prospects are critically discussed to assist researchers in identifying the shortcomings of the existing biosensors and establishing new methods centered on the development of unique, efficient, sensitive and selective GQDs-based biosensors.