Synthesis, characterization and biological applications of mycosynthesized silver nanoparticles

Sathiyaseelan, Anbazhagan; Shajahan, A.; Morukattu, Girilal; Rajan, Ramachandran; Kaviyarasan, V.; Thangavelu, K.

doi:10.1007/s13205-017-0961-9

Cited by 33 publications

(17 citation statements)

References 23 publications

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“…El-Naggar et al [19] prepared phycocyanin-reduced AgNPs that had an IC 50 value of 27.79 ± 2.3 µg/mL against mammary gland breast cancer (MCF-7), 31.78 ± 2.2 µg/mL against human lung fibroblast (WI38) cell line, and 32.97 ± 1.7 µg/mL against human amnion (WISH) cell lines. Similarly, Anbazhagan et al [50] synthesized AgNPs using Cunninghamella echinulata and tested their cytotoxicity against Vero cell lines, which showed an IC 50 value of 62.8 µg/mL for AgNPs. This activity was contrasted against the cytotoxic activity of AgNO 3 , which indicated that myco-synthesized nanoparticles were less cytotoxic to normal cells than bulk salts.…”

Section: Resultsmentioning

confidence: 99%

Biosynthesis of Silver Nanoparticles Mediated by Extracellular Pigment from Talaromyces purpurogenus and Their Biomedical Applications

et al. 2019

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In recent years, green syntheses have been researched comprehensively to develop inexpensive and eco-friendly approaches for the generation of nanoparticles. In this context, plant and microbial sources are being examined to discover potential reducing agents. This study aims to utilize an extracellular pigment produced by Talaromyces purpurogenus as a prospective reducing agent to synthesize silver nanoparticles (AgNPs). Biosynthesized AgNPs were characterized by transmission electron microscopy (TEM), dynamic light scattering (DLS), electron probe micro analyser (EPMA), and zeta potential. The pigment functional groups involved in the generation of AgNPs were investigated using Fourier transform infrared spectroscopy. TEM images showed that the generated nanoparticles were spherical, hexagonal, rod-shaped, and triangular-shaped with a particle size distribution from 4 to 41 nm and exhibited a surface plasmon resonance at around 410 nm. DLS and zeta potential studies revealed that the particles were polydispersed and stable (−24.8 mV). EPMA confirmed the presence of elemental silver in the samples. Biosynthesized AgNPs exhibited minimum inhibitory concentrations of 32 and 4 μg/mL against E. coli and S. epidermidis, respectively. Further, cytotoxicity of the AgNPs was investigated against human cervical cancer (HeLa), human liver cancer (HepG2), and human embryonic kidney (HEK-293) cell lines using 5-fluorouracil as a positive control. A significant activity was recorded against HepG2 cell line with a half-maximal inhibitory concentration of 11.1 μg/mL.

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Section: Resultsmentioning

confidence: 99%

Biosynthesis of Silver Nanoparticles Mediated by Extracellular Pigment from Talaromyces purpurogenus and Their Biomedical Applications

et al. 2019

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“…The intensity of brown color and absorbance units increases with the increase of the incubation period. Therefore, 15 min was used for the synthesis of biogenic AgNPs, providing an easy way and rapid protocol, which was an important advantage of biological methods compared to other methods using different primary biological material because they are normally synthesized within 30 min or more 21,22,[34][35][36][37] . TEM showed a widespread www.nature.com/scientificreports/ distribution of biogenic AgNPs with spherical and quasi-spherical shapes.…”

Section: Discussionmentioning

confidence: 99%

“…There are studies and reports that plant extract NPs have strong antimicrobial activity. These AgNPs were effective in killing a range of bacterial pathogens involved in different infectious diseases, e.g., S. aureus, P. aeruginosa, E. coli and C. albicans are related to most common hospital-acquired infections 36,42 . Our biogenic AgNPs possessed higher antimicrobial activity than other biogenic AgNPs 40,41 .…”

Section: Discussionmentioning

confidence: 99%

Green synthesis of silver nanoparticles using Lysiloma acapulcensis exhibit high-antimicrobial activity

Garibo

Borbón-Nuñez

León

et al. 2020

Sci Rep

356

121

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The scientific community is exploiting the use of silver nanoparticles (AgNPs) in nanomedicine and other AgNPs combination like with biomaterials to reduce microbial contamination. In the field of nanomedicine and biomaterials, AgNPs are used as an antimicrobial agent. One of the most effective approaches for the production of AgNPs is green synthesis. Lysiloma acapulcensis (L. acapulcensis) is a perennial tree used in traditional medicine in Mexico. This tree contains abundant antimicrobial compounds. In the context of antimicrobial activity, the use of L. acapulcensis extracts can reduce silver to AgNPs and enhance its antimicrobial activity. In this work, we demonstrate such antimicrobial activity effect employing green synthesized AgNPs with L. acapulcensis. The FTIR and LC-MS results showed the presence of chemical groups that could act as either (i) reducing agents stabilizing the AgNPs or (ii) antimicrobial capping agents enhancing antimicrobial properties of AgNPs. The synthesized AgNPs with L. acapulcensis were crystalline with a spherical and quasi-spherical shape with diameters from 1.2 to 62 nm with an average size diameter of 5 nm. The disk diffusion method shows the magnitude of the susceptibility over four pathogenic microorganisms of clinical interest. The antimicrobial potency obtained was as follows: E. coli ≥ S. aureus ≥ P. aeruginosa > C. albicans. The results showed that green synthesized (biogenic) AgNPs possess higher antimicrobial potency than chemically produced AgNPs. The obtained results confirm a more significant antimicrobial effect of the biogenic AgNPs maintaining low-cytotoxicity than the AgNPs produced chemically. The field of material sciences encourages to obtain materials of various types of nanoscale shapes and architectures 1 .NPs with a size range of 1-100 nm, and different shapes provide unique chemical 2 , physical 3 and optical properties 4,5. NPs can be synthesized with physical, chemical and biological methods 6. These methods might have unique advantages and disadvantages depending on the end application 7-11. For example, physical methods might have some disadvantages when applied in microbiology. The methods can be time-consuming and constrain to specific requirements like high temperature or pressure, which might result unattractive owing to equipment and associated cost 12,13. A key advantage of chemical methods is the accessibility to get the NPs in

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“…Metallic NPs consisting of one metal, metal oxides or a composite of several metals play a significant role in nanotechnology due to their potential utilization in industry and medicine [3][4][5][6][7]. NPs of noble metals possess low cytotoxicity, enable easy modification of their surfaces, have straightforward synthesis processes and excellent biocompatibility [8][9][10]. Such advantages make metallic NPs obtained by green synthesis (gNPs) prospective for applications in biological analysis, drug delivery and imaging, environmental monitoring, industrial catalysis and electronic devices [11][12][13][14][15][16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

“…Many plants [11,12,21,[24][25][26], algae [4], microorganisms [1,3,[7][8][9][10]14,18,23,[27][28][29][30], as well as redox-imbalanced mammalian cells and systems [31] are known to produce nanostructured mineral crystals and metallic NPs with properties similar to chemically-synthesized materials. Biological agents, including polysaccharides, polypeptides, DNA, enzymes secreted by cells [24,[32][33][34][35][36][37][38][39][40][41] and purified enzymes [42,43] are able to reduce noble metal ions to gain metallic NPs.…”

Section: Introductionmentioning

confidence: 99%

Metallic Nanoparticles Obtained via “Green” Synthesis as a Platform for Biosensor Construction

et al. 2019

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Novel nanomaterials, including metallic nanoparticles obtained via green synthesis (gNPs), have a great potential for application in biotechnology, industry and medicine. The special role of gNPs is related to antibacterial agents, fluorescent markers and carriers for drug delivery. However, application of gNPs for construction of amperometric biosensors (ABSs) is not well documented. The aim of the current research was to study potential advantages of using gNPs in biosensorics. The extracellular metabolites of the yeast Ogataea polymorpha were used as reducing agents for obtaining gNPs from the corresponding inorganic ions. Several gNPs were synthesized, characterized and tested as enzyme carriers on the surface of graphite electrodes (GEs). The most effective were Pd-based gNPs (gPdNPs), and these were studied further and applied for construction of laccase- and alcohol oxidase (AO)-based ABSs. AO/GE, AO-gPdNPs/GE, laccase/GE and laccase-gPdNPs/GE were obtained, and their analytical characteristics were studied. Both gPdNPs-modified ABSs were found to have broader linear ranges and higher storage stabilities than control electrodes, although they are less sensitive toward corresponding substrates. We thus conclude that gPdNPs may be promising for construction of ABSs for enzymes with very high affinities to their substrates.

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Synthesis, characterization and biological applications of mycosynthesized silver nanoparticles

Cited by 33 publications

References 23 publications

Biosynthesis of Silver Nanoparticles Mediated by Extracellular Pigment from Talaromyces purpurogenus and Their Biomedical Applications

Biosynthesis of Silver Nanoparticles Mediated by Extracellular Pigment from Talaromyces purpurogenus and Their Biomedical Applications

Green synthesis of silver nanoparticles using Lysiloma acapulcensis exhibit high-antimicrobial activity

Metallic Nanoparticles Obtained via “Green” Synthesis as a Platform for Biosensor Construction

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