Synthesis, characterization and concentration dependant antibacterial potentials of nickel oxide nanoparticles against Staphylococcus aureus and Escherichia coli

Abstract

Bacterial resistance to antibiotic treatment is a major emerging clinical and public health issue across the globe. Advancements in the field of metal oxide nanomaterials in the last few years have improved the potential of metal oxides in different applications. Metal oxides, of which, nickel oxide (NiO) is one, also possess antibacterial activities. This investigation was planned to synthesize NiO nanoparticles to study their antibacterial potential in comparison with bulk NiO and standard antibiotics at different concentrations. Synthesis and characterization of NiO nanoparticles was done by standard procedures. The antibacterial potentials of different compounds were determined at different concentrations against S. aureus and E. coli. The diameter of zone of inhibition showed that the antibacterial effect of NiO nanoparticles against S. aureus was better than E. coli at the same concentration. The concentration-dependent effect of NiO nanoparticles was observed from 0.125 to 128 µg/ml. The effect of NiO nanoparticles was markedly better than bulk NiO at all concentrations. Tetracycline and gentamicin did not show effect below 1.0 µg/ml and 2.0 µg/ml, respectively. The activity index and fold increase of NiO nanoparticles were both higher than 1 and positive, with respect to tetracycline, gentamicin and bulk NiO against S. aureus and E. coli at all the tested concentrations. In conclusion, the NiO nanoparticles seemed to be a more potent antibacterial agent than their bulk form, tetracycline and gentamicin, and in future, their applications may be extended in biomedical field and other areas to reduce microbial infections and incidences of antibacterial resistance.

Silver Nanoparticle-Based Combinations with Antimicrobial Agents against Antimicrobial-Resistant Clinical Isolates

“…Another study found that the second most frequently isolated AMR bacteria from Saudi hospitals were K. pneumoniae , with a significant percentage being ESBL-positive [ 16 ]. As a result, clinicians have had to consider treatment regimens based on combinations of medications with inadequate activity or to reuse some old and abandoned antimicrobials, such as colistin, that are known to have several adverse reactions [ 17 , 18 , 19 , 20 ]. As a result, the development of novel antimicrobial agents is critically warranted to fight these microorganisms with minimal and tolerated toxicity.…”

Chemical Synthesis and Antipseudomonal Activity of Al-Doped NiO Nanoparticles

“…The antibacterial activity of pure-NiO nanoparticles against Gram-negative bacteria (Pseudomonas aeruginosa, Escherichia coli) and Gram-Positive bacteria (Staphylococcus aureus, Streptococcus pneumonia) has been reported (Baek and An, 2011). NiO nanoparticles were found better antibacterial agents than tetracycline and gentamicin antibiotics against Gram-negative and Gram-positive bacteria (Gupta et al, 2020a). The antibacterial activity of NiO nanoparticles using different antimicrobial and biophysical studies revealed that these nanoparticles have stronger antibacterial activity against Gram-positive bacteria compared to Gram-negative bacteria (Behera et al, 2019).…”