Synthesis, characterization and DNA interactions of [Pt3(TPymT)Cl3], the trinuclear platinum(II) complex of the TPymT ligand

Abstract

The triplatinum complex of the 2,4,6-Tris(2-pyrimidyl)-1,3,5-triazine ligand, PtTPymT hereafter, has been prepared and characterized for the first time. NMR studies point out that the three platinum(II) centers possess an identical coordination environment. The interactions of PtTPymT with DNA were explored in comparison to the free ligand. Specifically, fluorescence, mass spectrometry, viscometry and melting measurements were carried out. In contrast to expectations, the obtained data reveal that no intercalative binding takes place; we propose that binding of PtTPymT to DNA mainly occurs through external/groove binding.

Exploring the Anticancer Potential of Diiron Bis-cyclopentadienyl Complexes with Bridging Hydrocarbyl Ligands: Behavior in Aqueous Media and In Vitro Cytotoxicity

“…Ultrapure water (Sartorius) was the reaction medium together with a NaCl 0.1 M + NaCac (sodium cacodylate) aqueous buffer used to maintain pH 7.0. In EB/DNA exchange experiments, DNA was saturated with EB according to a known procedure; in brief, EB was added to DNA until the fluorescence emission increase at the excitation/emission wavelength selective for the EB/DNA intercalated complex faded out ( T = 25.0 °C, C DNA = 1.30 × 10 –4 M, C EB = 5.33 × 10 –5 M, C DNA / C EB = 2.4, λ ex = 520 nm, λ em = 595 nm). Subsequently, increasing amounts of the stock metal complex solution were added to the EB/DNA mixture (Figure S1).…”

Diiron Complexes with a Bridging Functionalized Allylidene Ligand: Synthesis, Structural Aspects, and Cytotoxicity

“…Fluorescence measurements were performed on a PerkinElmer LS55 instrument (temperature ±0.1 °C). In EtBr/DNA exchange experiments, DNA was saturated with EtBr according to a documented procedure: 46 i.e., until the end of the fluorescence emission increase at the excitation/emission wavelength selective for the EtBr/DNA intercalated complex (λ ex = 520 nm, λ em = 595 nm). Subsequently, increasing amounts of 2b stock solution were added to the EtBr/DNA mixture.…”

s‐Triazine pincer ligands: Synthesis of their metal complexes, coordination behavior, and applications

“…and the [(TPymT)Pt 3 (Cl) 3 ](OH) 3 complex toward interaction with DNA was carried out via viscometry, mass spectrometry, melting, and fluorescence measurements. The results showed that there is no intercalative binding between the DNA and [(TPymT)Pt 3 (Cl) 3 ](OH) 3 complex 195 [149] [150] The hydrolysis of TPymT in presence of copper sulfate or perchlorate into the corresponding bis (2-pyrimidylcarbonyl)amidate (bpcam) were reported by de Castro Gomes et al in 2005. The copper complexes were separated either as mononuclear or dinuclear complexes in a distorted square pyramidal coordination geometry (Scheme 74).…”

“…and the [(TPymT)Pt 3 (Cl) 3 ](OH) 3 complex toward interaction with DNA was carried out via viscometry, mass spectrometry, melting, and fluorescence measurements. The results showed that there is no intercalative binding between the DNA and [(TPymT)Pt 3 (Cl) 3 ](OH) 3 complex 195 [149] (Scheme 72).…”