Synthesis, characterization and in vitro cytotoxicity studies of poly-N-isopropyl acrylamide gel nanoparticles and films

Litowczenko, Jagoda; Gapiński, Jacek; Markiewicz, Roksana; Woźniak, Anna; Wychowaniec, Jacek K.; Peplińska, Barbara; Jurga, Stefan; Patkowski, A.

doi:10.1016/j.msec.2020.111507

Cited by 16 publications

(8 citation statements)

References 33 publications

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“…It was reported that the hydrophobicity of pNIPAM could affect the adhesion of cells of different origins due to its capacity to adsorb matrix proteins (i.e., fibronectin) and growth factors stimulating cell growth [ 48 ]. The dependence of cell behavior on the type of cell line used in biological experiments evaluating pNIPAM copolymer was also reported by others [ 33 , 49 , 50 ].…”

Section: Resultssupporting

confidence: 73%

“…In our study, all the p(NIPAM-BA) coatings showed hydrophilic surfaces, with the lowest hydrophilicity exhibited by the layers obtained by MAPLE and SC deposition procedures and chloroform solvent ( Figure 8 ), where a significant reduction in cell viability was observed, especially at 48 h for both cell lines ( Figure 9 A,B). Roughness also influences cell behavior; cells such as fibroblasts prefer smooth surfaces, while osteoblasts adhere better on coatings with higher roughness [ 18 , 50 , 51 , 52 , 53 , 54 ]. In our case, MAPLE layers using DMSO exhibit the lowest roughness compared to H 2 O and chloroform and together with their hydrophilic character conducted a better survival of normal cells compared to melanoma cells.…”

Section: Resultsmentioning

confidence: 99%

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New Poly(N-isopropylacrylamide-butylacrylate) Copolymer Biointerfaces and Their Characteristic Influence on Cell Behavior In Vitro

Dumitrescu

Icriverzi

Bonciu

et al. 2022

IJMS

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Designing and obtaining new synthetic smart biointerfaces with specific and controlled characteristics relevant for applications in biomedical and bioengineering domains represents one of the main challenges in these fields. In this work, Matrix-Assisted Pulsed Laser Evaporation (MAPLE) is used to obtain synthetic biointerfaces of poly(N-isopropyl acrylamide-butyl acrylate) p(NIPAM-BA) copolymer with different characteristics (i.e., roughness, porosity, wettability), and their effect on normal HEK 293 T and murine melanoma B16-F1 cells is studied. For this, the influence of various solvents (chloroform, dimethylsulfoxide, water) and fluence variation (250-450 mJ/cm2) on the morphological, roughness, wettability, and physico–chemical characteristics of the coatings are evaluated by atomic force microscopy, scanning electron microscopy, contact angle measurements, Fourier-transform-IR spectroscopy, and X-ray photoelectron spectroscopy. Coatings obtained by the spin coating method are used for reference. No significant alteration in the chemistry of the surfaces is observed for the coatings obtained by both methods. All p(NIPAM-BA) coatings show hydrophilic character, with the exception of those obtained with chloroform at 250 mJ/cm2. The surface morphology is shown to depend on both solvent type and laser fluence and it ranges from smooth surfaces to rough and porous ones. Physico–chemical and biological analysis reveal that the MAPLE deposition method with fluences of 350-450 mJ/cm2 when using DMSO solvent is more appropriate for bioengineering applications due to the surface characteristics (i.e., pore presence) and to the good compatibility with normal cells and cytotoxicity against melanoma cells.

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Section: Resultssupporting

confidence: 73%

Section: Resultsmentioning

confidence: 99%

New Poly(N-isopropylacrylamide-butylacrylate) Copolymer Biointerfaces and Their Characteristic Influence on Cell Behavior In Vitro

Dumitrescu

Icriverzi

Bonciu

et al. 2022

IJMS

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“…Both primary HUVEC cells and HeLa cells exhibited their proper morphologies, similar to those recently reported. [ 43 ] As shown in Figure 3 , HUVEC cells exhibited proper endothelial cell phenotype characteristics, including the cytoskeletal structure and an elongated cell morphology. HeLa cells also had typical morphology, with an almost circular shape.…”

Section: Resultsmentioning

confidence: 99%

Nanomolar Nitric Oxide Concentrations in Living Cells Measured by Means of Fluorescence Correlation Spectroscopy

et al. 2022

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Measurement of the nitric oxide (NO) concentration in living cells in the physiological nanomolar range is crucial in understanding NO biochemical functions, as well as in characterizing the efficiency and kinetics of NO delivery by NO-releasing drugs. Here, we show that fluorescence correlation spectroscopy (FCS) is perfectly suited for these purposes, due to its sensitivity, selectivity, and spatial resolution. Using the fluorescent indicators, diaminofluoresceins (DAFs), and FCS, we measured the NO concentrations in NO-producing living human primary endothelial cells, as well as NO delivery kinetics, by an external NO donor to the immortal human epithelial living cells. Due to the high spatial resolution of FCS, the NO concentration in different parts of the cells were also measured. The detection of nitric oxide by means of diaminofluoresceins is much more efficient and faster in living cells than in PBS solutions, even though the conversion to the fluorescent form is a multi-step reaction.

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“…[18] On the other hand, studies reported by Vihola et al do not demonstrate evidence of cytotoxicity by MTT and lactate dehydrogenase (LDH) tests when intestinal Caco-2 cells and bronchial Calu-3 cells were cultured in contact with PNIPAM. [16] As well as, dermal fibroblast MSU-1.1, cervical cancer cells HeLa, human umbilical vein endothelial cells, [29] and myoblasts C2C12 adhered to PNIPAM thin films did not show cytotoxic effects for 72 h. [30]…”

Section: Cytotoxicity and Cell Proliferation Studiesmentioning

confidence: 99%

Biointerfacial Analysis of HEK293 Cells in Contact with Hydrogels based on Poly‐N‐Isopropylacrylamide and Copolymers

Capella

Liaudat

Broglia

et al. 2022

Adv Materials Inter

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Several researches have demonstrated that synthetic hydrogels can mimic the mechanical and physicochemical properties of native extracellular matrices and act as cell‐scaffold. The biointerface can influence over tissue activities such as adhesion, signaling, cell–cell communications, and proliferation. In this work, the behavior of human embryonic kidney cells (HEK293) in contact with hydrogel surfaces based on poly‐N‐isopropylacrylamide (PNIPAM) and copolymers is studied. Ionic and neutral hydrogel surfaces are synthesized by free radical polymerization and characterized by Fourier‐transform infrared spectroscopy, swelling capacity, and wettability at culture conditions. Viability, proliferation, and bioadhesive capacity are analyzed by 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide and Neutral Red assays, [3H]‐thymidine technique, Acridine Orange and Hoechst dye. Noncytotoxic and proliferative effects are observed in all cases. HEK293 cells are mainly adapted and adhered on neutral and low ionic charge surfaces showing typical cell morphology, and normal mitotic rates. While, lower adhesion, abnormal nuclear and cytoplasmic morphologies with mitotic/fragmentation processes are observed after contact with high ionic surfaces. The cellular cycle after cultive on PNIPAM, assessed by flow cytometry, is not affected regarding control surfaces (polystyrene). Therefore, hydrogel surfaces with neutral and low ionic charges can be apt to in vitro tissue development and possible applications of biomedical treatments.

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Synthesis, characterization and in vitro cytotoxicity studies of poly-N-isopropyl acrylamide gel nanoparticles and films

Cited by 16 publications

References 33 publications

New Poly(N-isopropylacrylamide-butylacrylate) Copolymer Biointerfaces and Their Characteristic Influence on Cell Behavior In Vitro

New Poly(N-isopropylacrylamide-butylacrylate) Copolymer Biointerfaces and Their Characteristic Influence on Cell Behavior In Vitro

Nanomolar Nitric Oxide Concentrations in Living Cells Measured by Means of Fluorescence Correlation Spectroscopy

Biointerfacial Analysis of HEK293 Cells in Contact with Hydrogels based on Poly‐N‐Isopropylacrylamide and Copolymers

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