Synthesis, characterization and RNA-binding properties of a novel ruthenium(II) complex coordinated by 5-pyridine-10,15,20-triphenylporphyrin

Abstract

A novel ruthenium(II) complex, [dibipyridyl-(5-pyridine-10,15,20-tri-phenylporphyrin)] ruthenium(II) chloride, (1), has been synthesized and characterized by elementary analyzing, ESI-MS and 13 C-NMR, as well as spectroscopy. The electronic spectra and emission spectra have been utilized to study the interaction of this ruthenium(II) complex (1) with the total RNA of liver cells, and the results show that there was obviously hypochromism and a red shift was observed for the Soret absorption and IL transition in electronic spectra of ruthenium(II) complex (1) in the presence of total RNA of liver cells, and the hypochromism of Soret absorption and IL absorption of complex (1) is 25% and 10%, respectively. The studies on the steady-state emission spectra of complex (1) show that the fluorescent of (1) in the 550-700 nm range increased obviously in the presence of total RNA of liver cells. These data, together with that of electronic spectra, shown that the ruthenium(II) complex (1) can bind to RNA excellently.

Effect of hydrophobicity on intercalative binding of some surfactant copper(II) complexes with tRNA

“…In contrast to DNA, very small attention has been paid to the binding properties of RNA with transition metal complexes. Plenty of work pertaining to the RNA-binding properties [6][7][8], catalysts of RNA hydrolysis cleavage [9], shape-selective probes of RNA tertiary structure [10], agents of RNA oxidation cleavage [11], and approval of mismatches in RNA [12,13] has also been reported, but the RNA-binding properties of surfactant copper(II) complexes remain very fragmentary as compared to their DNA binding studies. To deeply understand the RNA-binding properties and concede important fundamental data for RNA-targeting drugs, much work is required.…”

Interaction of polyethyleneimine‐anchored copper(II) complexes with tRNA studied by spectroscopy methods and biological activities

“…This interest is due to the unique physical properties of these complexes and their potential applications in biology. In particular, the potential application of these complexes in the design and development of synthetic restriction enzymes, new drugs, DNA footprinting agents and stereoselective probes of nucleic acid structure has been explored extensively . However, there are few reports pertaining to interactions between transition metal complexes and transfer RNA (tRNA) .…”

“…In particular, the potential application of these complexes in the design and development of synthetic restriction enzymes, new drugs, DNA footprinting agents and stereoselective probes of nucleic acid structure has been explored extensively . However, there are few reports pertaining to interactions between transition metal complexes and transfer RNA (tRNA) . The future development of tRNA‐targeting drugs relies on a deeper understanding of these binding processes, leading to advances in medical applications, such as the inhibition of HIV and photodynamic therapy for tumours .…”

Studies on the interactions of polymer-anchored copper(II) complexes with tRNA

“…These metal complexes have been used as catalysts of RNA hydrolysis cleavage [12], shape-selective probes of RNA tertiary structure [13], agents of RNA oxidation cleavage [14] and for recognition of mismatches in RNA [15]. But only a few reports have investigated the interactions between transition metal complexes and RNA [16][17][18]. The future development of RNA-targeting drugs will rely on a deeper understanding of these binding processes, leading to advances in medical applications such as the inhibition of the AIDS virus and the photodynamic therapy of tumours [19].…”