Synthesis, characterization and spectroscopic studies of surfactant loaded antiulcer drug into Chitosan nanoparticles for interaction with bovine serum albumin

Qashqoosh, Mohsen T.A.; Alahdal, Faiza A.M.; Manea, Yahiya Kadaf; Zakariya, Syed Mohammad; Naqvi, Saba

doi:10.1016/j.chemphys.2019.110462

Cited by 23 publications

(7 citation statements)

References 65 publications

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“…The suspensions of CFX-loaded C.S./PS-TPP were centrifuged at 5000 rpm for 20 min, and the supernatant liquid was determined spectrophotometrically, allowing the determination of the drug entrapment efficiency . The drug entrapment efficiency (DEE %) was estimated using the following equation: DEE 0.25em ( % ) = normala normalmount of drug actually present ⁢ 0.25em ( μ g ) normalt normalheoretical drug loa normald expected × 100 …”

Section: Methodsmentioning

confidence: 99%

In Vitro Hemoglobin Binding and Molecular Docking of Synthesized Chitosan-Based Drug-Carrying Nanocomposite for Ciprofloxacin-HCl Drug Delivery System

Manea,

Qashqoosh,

Rezakazemi

2024

ACS Omega

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Understanding the intermolecular interactions between antibiotic drugs and hemoglobin is crucial in biological systems. The current study aimed to investigate the preparation of chitosan/polysorbate-80/tripolyphosphate (CS-PS/TPP) nanocomposite as a potential drug carrier for Ciprofloxacin-HCl drug (CFX), intended for controlled release formulation and further used to interact with bovine hemoglobin. Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric analysis−differential thermal analysis (TGA-DTA), scanning electron microscopy (SEM), dynamic light scattering (DLS), and X-ray diffraction analyses were used to characterize the CS-PS/TPP nanocomposite and its CFX-loaded nanocomposite. The second series of biophysical properties were performed on the Ciprofloxacin-loaded CS-PS/TPP (NCFX) for interaction with bovine hemoglobin (BHb). The interactions of (CFX and NCFX) with redox protein hemoglobin were investigated for the first time through a series of in vitro experimental techniques to provide comprehensive knowledge of the drug−protein binding interactions. Additionally, the effect of inclusion of PS-80 on the CFX-BHb interaction was also studied at different concentrations using fluorescence spectroscopy, ultraviolet−visible (UV−Vis) spectroscopy, and circular dichroism (CD) under physiological conditions. The binding process of CFX and NCFX was spontaneous, and the fluorescence of BHb was quenched due to the static mechanism formation of the (CFX/BHb) and (NCFX/BHb) complexes. Thermodynamic parameters ΔG, ΔH, and ΔS at various temperatures indicate that the hydrogen bonding and van der Waals forces play a major role in the CFX-BHb association.

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Section: Methodsmentioning

confidence: 99%

In Vitro Hemoglobin Binding and Molecular Docking of Synthesized Chitosan-Based Drug-Carrying Nanocomposite for Ciprofloxacin-HCl Drug Delivery System

Manea,

Qashqoosh,

Rezakazemi

2024

ACS Omega

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“…The molecular docking is an excellent and an efficient computational technique, which is used to predict the probable binding site of interaction of protein with the ligand/drug molecule and also the preferred binding site of the ligand through the 3-D graphics [4,11,47,99]. Moreover, it also displays the amino acid residues encircling the ligand/drug molecule and also assists to validate the results and highlights the interaction types operating in the protein-ligand/drug system [4,99,100].…”

Section: Molecular Docking Analysismentioning

confidence: 99%

“…Recently, the nanoparticle studies have opened new avenues to study biomolecular interactions with their applications as drug delivery, biocompatibility, diagnostics, and smart materials. The binding of nanocolloidal particles to proteins has also been formed, since the study of immunoprobes in the early 1970s [11]. Moreover, various studies of peptide or protein including lysozyme binding with nanoparticles of different sizes have been conducted.…”

Section: Introductionmentioning

confidence: 99%

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Influence of Tween 80 Surfactant on the Binding of Roxatidine Acetate and Roxatidine Acetate–loaded Chitosan Nanoparticles to Lysozyme

Qashqoosh¹,

Alahdal²,

Manea³

et al. 2022

Surfactants and Detergents - Updates and New Insights

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The drug binding to protein is an attractive research topic. In order to assess the release of RxAc-CsNPs and their binding with lysozyme under physiological conditions, nanocomposite materials based on chitosan (Cs) and Roxatidine acetate (RxAc) in the presence Tween 80 (Tw80) surfactant were developed. The addition of Tw80 to CsNPs increased RxAc release in vitro. In this work, Stern–Volmer plot and thermodynamic results indicated that the mechanism of Lyz with RxAc and Lyz with RxAc-CsNPs was static mechanism and the main forces in both systems were hydrogen bonding and Van der Waals forces, which indicated that the binding reaction in both systems is spontaneous, exothermic and enthalpically driven. Synchronous fluorescence and CD results indicated that the RxAc and RxAc-CsNPs cause change in the secondary construction of Lyz. It was also found that the addition of Tw80 affects the binding constant of drug with protein. Finally, the molecular docking results have also been in accordance with the results of other techniques. Hence, the developed RxAc loaded Chitosan nanoparticles could be used as an effective strategy for designing and application of the antiulcer drugs. Altogether, the present study can provide an important insight for the future designing of antiulcer drugs.

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“…In addition to these drugs, a drug named lansoprazole (LPZ) as illustrated in Figure 1 , which is frequently used to treat peptic ulcers in a number of situations such as duodenal ulcers, benign gastric ulcers, gastroesophageal reflux disease, and also long-term Zollinger–Ellison syndrome, is of considerable research interest. Since the liver is the organ that metabolizes this medication most efficiently, 24 patients with renal and hepatic issues must take a lower dose. However, in some typical systems, a lower dose may not have the desired pharmacological effect.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of 2D Metal–Organic Nanosheets (MONs) by Liquid Phase Exfoliation: Applications in Effective Delivery of Antiulcer Drugs and Selective Adsorption and Removal of Cationic Dyes

et al. 2023

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Nowadays, the fabrication of 2D metal−organic nanosheets (2D MONs) has entered the research arena fascinating researchers worldwide. However, a lack of efficient and facile methods has remained a bottleneck for the manufacturing of these 2D MONs. Herein, a 2D metal−organic framework (MOF), i.e., 2D Cu-MOF, was synthesized using a facile and convenient stirring method by using 4,4′-trimethylenedipyridine (TMDP) as an organic linker. The as-prepared MOF was characterized in detail and based on single crystal X-ray diffraction analysis, it was established that tangled layers in the 2D Cu-MOF are interconnected to produce thick strands. These tangled layers could be easily separated via ultrasonication-induced liquid phase exfoliation (UILPE) to give the 2D Cu-MON as illustrated through Tyndall light scattering and exhaustive microscopic exploration such as scanning electron microscopy (SEM) and transmission electron microscopy (TEM). The application of this 2D Cu-MON was assessed in the field of drug delivery revealing exceptional drug loading for the drug lansoprazole (LPZ) by 2D Cu-MONs as well as drug release in the acidic and neutral medium demonstrating that the 2D Cu-MON is an excellent carrier for antiulcer drug delivery. For environmental protection, the application of 2D Cu-MON was also examined toward the removal of various cationic and anionic dyes with excellent selectivity toward cationic dye removal. The plausible mechanism for dye removal indicated the involvement of cation−π and π−π interactions, for the effective adsorption of cationic dyes as well as a increase in the surface area of 2D Cu-MON by UILPE. Remarkably, the high drug loading and dye removal are imputed to the increase in surface area by UILPE. In a nutshell, the developed 2D Cu-MON will prove to be beneficial for application in the field of drug delivery as well as for wastewater treatment.

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Synthesis, characterization and spectroscopic studies of surfactant loaded antiulcer drug into Chitosan nanoparticles for interaction with bovine serum albumin

Cited by 23 publications

References 65 publications

In Vitro Hemoglobin Binding and Molecular Docking of Synthesized Chitosan-Based Drug-Carrying Nanocomposite for Ciprofloxacin-HCl Drug Delivery System

In Vitro Hemoglobin Binding and Molecular Docking of Synthesized Chitosan-Based Drug-Carrying Nanocomposite for Ciprofloxacin-HCl Drug Delivery System

Influence of Tween 80 Surfactant on the Binding of Roxatidine Acetate and Roxatidine Acetate–loaded Chitosan Nanoparticles to Lysozyme

Synthesis of 2D Metal–Organic Nanosheets (MONs) by Liquid Phase Exfoliation: Applications in Effective Delivery of Antiulcer Drugs and Selective Adsorption and Removal of Cationic Dyes

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