Abstract:There is enormous interest in combining two or more nanoparticles for various biomedical applications, especially in anti-cancer agent delivery. In this study, the microsphere nanoparticles were prepared (MSNPs) and their impact on cancer cells was examined. The MSNPs were prepared by using the hydrothermal method where strontium (Sr), barium (Ba), dysprosium (Dy), samarium (Sm), and iron oxide (Fe8−2xO19) were combined, and dysprosium (Dy) and samarium (Sm) was substituted with strontium (Sr) and barium (Ba),… Show more
“…The current study’s findings are consistent with a previously published report. 26 , 27 Figure 15 Dose-dependent cytotoxic activity of NSFs (x=0.02 and x=0.03) toward HCT-116 cells estimated by MTT assay. Asterisks indicate significant differences between the viability of cells.…”
Section: Resultsmentioning
confidence: 99%
“…The findings of the current study are in line with those of the previous investigation. 26 , 28 , 37 Figure 16 Morphology of DAPI stained cancer cells after 48 h of treatment. ( A ) represents control, whereas ( B and C ) represent HCT-116 cells treated with 35 µg/mL of NSFs (x=0.02), and (x=0.03), respectively.…”
Section: Resultsmentioning
confidence: 99%
“…The cell viability activity and alteration in DNA of HCT-116 cell line after treatment with NSFs was examined by MTT and DAPI staining assay, respectively, according to previous report. 26 Briefly, the cells were treated with varying concentrations of NSFs (X=0.02 and x=0.03) in a 96 well culture plates. As a control group, cells were cultured without NSFs.…”
Background
The constant rise of microbial biofilm formation and drug resistance to existing antimicrobial drugs poses a significant threat to community health around the world because it reduces the efficacy and efficiency of treatments, increasing morbidity, mortality, and health-care expenditures. As a result, there is an urgent need to develop novel antimicrobial agents that inhibit microbial biofilm formation.
Methods
The [Ni
0.4
Cu
0.2
Zn
0.4
](Fe
2-x
Dy
x
)O
4
(x≤0.04) (Ni-Cu-Zn) nano spinel ferrites (NSFs) have been synthesized by the sol–gel auto-combustion process and were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive x-ray (EDX) and transmission electron microscopy (TEM). The antimicrobial, antibiofilm and antiproliferative activities of Ni-Cu-Zn NSFs were also examined.
Results
The XRD pattern confirms the secondary phase DyFeO
3
and Fe
2
O
3
for substituted Dy
3
+
samples, and the crystallite size ranged from 10 to 19 nm. TEM analysis of NSFs revealed that the particles were cube-shaped and 15nm in size. NSFs exhibited significant antimicrobial, antibiofilm and antiproliferative activity. At concentration of 1 mg/mL, it was found that the NSFs (ie, x=0.0, x=0.01, x=0.02, x=0.03 and x=0.04) inhibit biofilm formation by 27.6, 26.2, 58.5, 33.3 and 25% for methicillin-resistant
Staphylococcus aureus
(MRSA) and 47.5, 43.5, 48.6, 58.3 and 26.6% for
Candida albicans
, respectively. SEM images demonstrate that treating MRSA and
C. albicans
biofilms with NSFs significantly reduces cell adhesion, colonization and destruction of biofilm architecture and extracellular polymeric substances matrices. Additionally, SEM and TEM examination revealed that NSFs extensively damaged the cell walls and membranes of MRSA and
C. albicans
. Huge ultrastructural alteration such as deformation, disintegration and separation of cell wall and membrane from the cells was observed, indicating significant loss of membrane integrity, which eventually led to cell death. Furthermore, it was observed that NSF inhibited the cancer cell growth and proliferation of HCT-116 in a dose-dependent manner.
Conclusion
The current study demonstrated that the synthesized Ni-Cu-Zn NSFs could be used to develop potential antimicrobial surface coatings agents for a varieties of biomedical-related materials and devices in order to prevent the biofilms formation and their colonization. Furthermore, the enhanced antiproliferative properties of manufactured SNFs suggest a wide range of biomedical applications.
“…The current study’s findings are consistent with a previously published report. 26 , 27 Figure 15 Dose-dependent cytotoxic activity of NSFs (x=0.02 and x=0.03) toward HCT-116 cells estimated by MTT assay. Asterisks indicate significant differences between the viability of cells.…”
Section: Resultsmentioning
confidence: 99%
“…The findings of the current study are in line with those of the previous investigation. 26 , 28 , 37 Figure 16 Morphology of DAPI stained cancer cells after 48 h of treatment. ( A ) represents control, whereas ( B and C ) represent HCT-116 cells treated with 35 µg/mL of NSFs (x=0.02), and (x=0.03), respectively.…”
Section: Resultsmentioning
confidence: 99%
“…The cell viability activity and alteration in DNA of HCT-116 cell line after treatment with NSFs was examined by MTT and DAPI staining assay, respectively, according to previous report. 26 Briefly, the cells were treated with varying concentrations of NSFs (X=0.02 and x=0.03) in a 96 well culture plates. As a control group, cells were cultured without NSFs.…”
Background
The constant rise of microbial biofilm formation and drug resistance to existing antimicrobial drugs poses a significant threat to community health around the world because it reduces the efficacy and efficiency of treatments, increasing morbidity, mortality, and health-care expenditures. As a result, there is an urgent need to develop novel antimicrobial agents that inhibit microbial biofilm formation.
Methods
The [Ni
0.4
Cu
0.2
Zn
0.4
](Fe
2-x
Dy
x
)O
4
(x≤0.04) (Ni-Cu-Zn) nano spinel ferrites (NSFs) have been synthesized by the sol–gel auto-combustion process and were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive x-ray (EDX) and transmission electron microscopy (TEM). The antimicrobial, antibiofilm and antiproliferative activities of Ni-Cu-Zn NSFs were also examined.
Results
The XRD pattern confirms the secondary phase DyFeO
3
and Fe
2
O
3
for substituted Dy
3
+
samples, and the crystallite size ranged from 10 to 19 nm. TEM analysis of NSFs revealed that the particles were cube-shaped and 15nm in size. NSFs exhibited significant antimicrobial, antibiofilm and antiproliferative activity. At concentration of 1 mg/mL, it was found that the NSFs (ie, x=0.0, x=0.01, x=0.02, x=0.03 and x=0.04) inhibit biofilm formation by 27.6, 26.2, 58.5, 33.3 and 25% for methicillin-resistant
Staphylococcus aureus
(MRSA) and 47.5, 43.5, 48.6, 58.3 and 26.6% for
Candida albicans
, respectively. SEM images demonstrate that treating MRSA and
C. albicans
biofilms with NSFs significantly reduces cell adhesion, colonization and destruction of biofilm architecture and extracellular polymeric substances matrices. Additionally, SEM and TEM examination revealed that NSFs extensively damaged the cell walls and membranes of MRSA and
C. albicans
. Huge ultrastructural alteration such as deformation, disintegration and separation of cell wall and membrane from the cells was observed, indicating significant loss of membrane integrity, which eventually led to cell death. Furthermore, it was observed that NSF inhibited the cancer cell growth and proliferation of HCT-116 in a dose-dependent manner.
Conclusion
The current study demonstrated that the synthesized Ni-Cu-Zn NSFs could be used to develop potential antimicrobial surface coatings agents for a varieties of biomedical-related materials and devices in order to prevent the biofilms formation and their colonization. Furthermore, the enhanced antiproliferative properties of manufactured SNFs suggest a wide range of biomedical applications.
This study focusses on the fabrication of nano-carriers for delivery of ciprofloxacin through the nanoprecipitation process. This was done to examine the release of drug at the pH of stomach to find out the antibacterial action of ciprofloxacin loaded nanoparticles (NPs). Prepared NPs were characterized by Fourier Transform Infra-Red (FTIR) spectroscopy, Scanning Electron Microscopy (SEM), and particle size analyzer (PSA) techniques. Drug yield, loading, and sustained release was studied as function of time (up to 8 h). Antibacterial activity of ciprofloxacin loaded NPs were also determined against different gram-positive and gram-negative bacteria. Results revealed that nanoprecipitation is a suitable method for encapsulation of ciprofloxacin in poly(lactic-co-glycolic acid) PLGA NPs. The drug yield and drug loading were found to be 60%. The size range of NPs observed by PSA was in the range of 5.03–6.60 nm. It can be concluded that nanoformulation of ciprofloxacin loaded PLGA NPs can be used in stomach for longer period of time to enhance the bioavailability of the drug.
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