Synthesis, Characterization, Biological Activity and DNA Cleavage Studies on Tetrazole Imine Base and Their Metal Complexes - An Experimental and Theoretical Approach

Abstract

Novel Tetrazole imine and its binary Cu(II) and Ni(II) complexes were synthesized and characterized by employing spectro-analytical techniques viz; Mass, UV, IR, 1 H NMR, elemental analyses, thermo-analytical methods, SEM and magnetic susceptibility measurements. The equilibrium studies were carried out using pH-metric technique to get an insight of the number of dissociable protons and protonation sites in candidate compound. The pH-Metric studies were also carried out in presence of metal ions to establish the formation of corresponding metal complexes in solution. To understand the frontier orbitals in title imine compound suitable for metal ion coordination, the computational studies were carried out by employing HyperChem 7.5 tools. The energies of HOMO and LUMO frontier orbitals were computed with geometry optimized molecules. The antimicrobial studies carried out by screening the tetrazole imine base and their metal complexes against bacteria inferred positive results. The DNA cleavage studies on title compound and its metal complexes using PBR322 DNA indicated that the tetrazole imine base has no role in cleavage of DNA and in its metal complexes, only Cu (II) complex played marked role in cleavage.

Synthesis, Characterization and Biological Activity Studies on Novel 4-Chloro-2- [(1-Phenyl-1H-Tetrazol-5-Ylimino)-Methyl] Phenol and Its Metal Complexes

“…The tetrazole functionality plays an important role in medicinal chemistry, primarily due to its ability to serve as the bioequivalent (bioisostere) of the carboxylic acid group [6], and also the class of tetrazole compounds have been used both as anticancer and antimicrobial agents. Earlier work from our laboratory on Cu (II) complex of tetrazole imine base 2-((E)-(1-phenyl-1H-tetrazol-5-ylimino) methyl) phenol (PTMP) revealed its antimicrobial and DNA cleavage activity [7]. Keeping in view the various applications of tetrazoles and in continuation of work reported earlier, in the present investigation synthesis of 4-Chloro-2-[(1-phenyl-1H-tetrazol-5-ylimino)-methyl] phenol (Cl-PTMP), characterization, molecular properties and metal ion interaction studies were envisaged.…”