Synthesis, characterization, controlled release, and antibacterial studies of a novel streptomycin chitosan magnetic nanoantibiotic

Hussein‐Al‐Ali, Samer Hasan; Zowalaty, Mohamed E. El; Hussein, Mohd Zobir; Ismail, Maznah; Webster, Thomas J.

doi:10.2147/ijn.s53079

Cited by 43 publications

(18 citation statements)

References 40 publications

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“…Figure 1 shows the [7]. The samples did not show additional peaks or phases suggesting that the modified MagNPs did not suffer any structural change [8,9]. 3 Journal of Nanomaterials C-N bonds from N(CH 2 ) 2 , respectively [13].…”

Section: Resultsmentioning

confidence: 98%

Synthesis of Poly(N-vinylcaprolactam)-Grafted Magnetite Nanocomposites for Magnetic Hyperthermia

Morfin-Gutierrez

Meléndez‐Ortiz²,

Puente-Urbina

et al. 2018

Journal of Nanomaterials

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In this study, the synthesis, characterization, and application of poly(N-vinylcaprolactam)-grafted magnetite nanocomposites for magnetic hyperthermia are reported. Superparamagnetic magnetite nanoparticles (MagNPs) with sizes in the range of 10–16 nm were synthesized by the coprecipitation method and then functionalized with vinyltrimethoxysilane (VTMS). MagNPs-VTMS coated with poly(N-vinylcaprolactam) (PNVCL) were prepared by free radical polymerization. The obtained materials were characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), vibration sample magnetometry (VSM), and Fourier transform infrared spectroscopy (FT-IR). The heating ability was evaluated under a magnetic field using a solid state induction heating equipment at 10.2 kA/m and 362 kHz. The MagNPs-PNVCL nanocomposites showed a behavior close to superparamagnetic materials, which is appropriated for magnetic hyperthermia treatment; in concentrations of 8 mg/mL, they were able to heat up, increasing the temperature up to 42°C in a period of time lower than 10 minutes.

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Section: Resultsmentioning

confidence: 98%

Synthesis of Poly(N-vinylcaprolactam)-Grafted Magnetite Nanocomposites for Magnetic Hyperthermia

Morfin-Gutierrez

Meléndez‐Ortiz²,

Puente-Urbina

et al. 2018

Journal of Nanomaterials

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“…Figure 3 panels C and D show the thermogravimetric analysis results for the aminosilane-, glutaraldehyde-, and CSA-13- functionalized magnetic nanoparticles, respectively. Bare MNP shows 6% weight loss in the temperature range 50–800°C, which is directly associated with the removal of adsorbed water and decomposition of hydroxyl surface groups [ 24 ]. The thermogravimetric analysis curves (Figure 3 C) show that the weight loss for aminosilane- and glutaraldehyde-coated MNPs during the performed analysis is about 8%.…”

Section: Resultsmentioning

confidence: 99%

“…Novel formulations using lipids and polymeric nanostructures for delivery of antimicrobial agents may be useful in other applications as well [ 29 ]. A magnetic drug delivery system was previously proposed for antibiotics such as chloramphenicol (successfully loaded into OA/SDS-coated MNPs) [ 30 ], nystatin [ 31 ] and streptomycin [ 24 ], both loaded on chitosan coated MNPs; however, the attaching process was achieved by chemical adsorption, based on non-specific electrostatic or hydrophobic interactions. The covalent immobilization of antibiotics proposed here possesses some advantages compared to chemical adsorption.…”

Section: Resultsmentioning

confidence: 99%

Bactericidal activity and biocompatibility of ceragenin-coated magnetic nanoparticles

et al. 2015

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BackgroundCeragenins, synthetic mimics of endogenous antibacterial peptides, are promising candidate antimicrobial agents. However, in some settings their strong bactericidal activity is associated with toxicity towards host cells. To modulate ceragenin CSA-13 antibacterial activity and biocompatibility, CSA-13-coated magnetic nanoparticles (MNP-CSA-13) were synthesized. Transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC) and thermogravimetric analysis (TGA) were used to characterize MNP-CSA-13 physicochemical properties. Bactericidal action and ability of these new compounds to prevent Pseudomonas. aeruginosa biofilm formation were assessed using a bacteria killing assay and crystal violet staining, respectively. Release of hemoglobin from human red blood cells was measured to evaluate MNP-CSA-13 hemolytic activity. In addition, we used surface activity measurements to monitor CSA-13 release from the MNP shell. Zeta potentials of P. aeruginosa cells and MNP-CSA-13 were determined to assess the interactions between the bacteria and nanoparticles. Morphology of P. aeruginosa subjected to MNP-CSA-13 treatment was evaluated using atomic force microscopy (AFM) to determine structural changes indicative of bactericidal activity.ResultsOur studies revealed that the MNP-CSA-13 nanosystem is stable and may be used as a pH control system to release CSA-13. MNP-CSA-13 exhibits strong antibacterial activity, and the ability to prevent bacteria biofilm formation in different body fluids. Additionally, a significant decrease in CSA-13 hemolytic activity was observed when the molecule was immobilized on the nanoparticle surface.ConclusionOur results demonstrate that CSA-13 retains bactericidal activity when immobilized on a MNP while biocompatibility increases when CSA-13 is covalently attached to the nanoparticle.

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“…Once bacterial cells adhere to the surface of the implants, bacteria can form a biofilm, which acts as a barrier to host defense mechanisms and resists to antimicrobial agents 40, 41 . CH and its derivatives have received significant scientific interests in surface modification for antibacterial, medical and pharmaceutical applications 30, 42–44 . Due to the presence of CH in CH-Ti and CH-MTX-Ti substrates, these substrates show significant resistance to the adhesion of S. aureus (Fig.…”

Section: Discussionmentioning

confidence: 99%

Chemical functionalization of bone implants with nanoparticle-stabilized chitosan and methotrexate for inhibiting both osteoclastoma formation and bacterial infection

et al. 2014

J. Mater. Chem. B

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A great challenge in orthopedic tumor operation faced by orthopedic implants is the high recurrence and metastasis of bone tumor as well as the bacterial infection associated with the implants. Thus ideal titanium (Ti)-based bone implants should be able to not only inhibit cancer cell adhesion and proliferation, promote cancer cell apoptosis, but also resist bacterial infections. Towards this end, we developed a new approach to modify the surface of Ti-based bone implants so that they can restrain functions of osteoclastoma (Giant cell tumor of bone) cancer cells (GCTs) and inhibit the adhesion of bacteria. First, the surface of pristine Ti substrates was functionalized with dopamine (DA) to form DA-Ti substrates. Then nanoparticles electrostatically assembled from poly-lysine (PLL) and heparin (Hep) were chemically immobilized onto the DA-Ti substrates to form PLL/Hep-Ti substrates. Chitosan (CH) and methotrexate (MTX) were then electrostatically immobilized onto the PLL/Hep-Ti substrates to generate CH-MTX-Ti substrates. The successful functionalization of the Ti substrates was confirmed by X-ray photoelectron spectroscopy. GCTs cultured on differently functionalized Ti substrates were investigated in terms of cell adhesion, cytoskeleton, proliferation, cytotoxicity and apoptosis. The growth of Staphylococcus aureus bacteria in the presence of different substrates was also assayed. Our results showed that CH-MTX-Ti substrates not only significantly inhibited the adhesion, proliferation and viability of GCTs, promoted the apoptosis of GCTs, but also prevented the adhesion of the bacteria and the subsequent formation of bacterial biofilms, when compared to other Ti substrates. Thus CH-MTX-Ti substrates are expected to be used as orthopedic prostheses in bone tumor surgery that can inhibit both osteoclastoma formation and bacterial infections.

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Synthesis, characterization, controlled release, and antibacterial studies of a novel streptomycin chitosan magnetic nanoantibiotic

Cited by 43 publications

References 40 publications

Synthesis of Poly(N-vinylcaprolactam)-Grafted Magnetite Nanocomposites for Magnetic Hyperthermia

Synthesis of Poly(N-vinylcaprolactam)-Grafted Magnetite Nanocomposites for Magnetic Hyperthermia

Bactericidal activity and biocompatibility of ceragenin-coated magnetic nanoparticles

Chemical functionalization of bone implants with nanoparticle-stabilized chitosan and methotrexate for inhibiting both osteoclastoma formation and bacterial infection

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