Synthesis, Characterization of a Baicalin‐Strontium(II) Complex and Its BSA‐Binding Activity

Abstract

Baicalin is one of the major bioactive constituents of Scutellaria baicalensis Georgi, possessed anti‐inflammation, antimicrobial and anticancer activity, has been attained extensive attention. In the present work, a new baicalin‐Sr(II) complex (Sr[baic]⋅3H2O) was synthesized to enhance its biological activity. A series of experiments were carried out to give the satisfying reaction conditions: pH=8.3 and the molar ratio of L:M ≤ 1:1. The obtained complex Sr[baic]⋅3H2O was characterized by IR, ES‐MS UV‐vis, atomic absorption, elemental analysis and thermogravimetric analysis. The interactions between baicalin/Sr[baic] and bovine serum albumin were also researched through fluorescence spectrum, which accord with the static quenching mechanism, Sr[baic] has higher binding ability than baicalin. Thermodynamic parameters ΔG°<0, ΔH°<0 and ΔS°>0, indicated that electrostatic forces is the major interaction forces in Sr[baic]‐bovine serum albumin system. Furthermore, the synchronous fluorescence experiments were carried out. The studies revealed that both baicalin and Sr[baic] quench the intrinsic fluorescence of bovine serum albumin, and the possible binding site is located on Trp residues. In circular dichroism spectrum, the binding of baicalin and Sr[baic] to bovine serum albumin lead to a loss in helical stability, by contrast, Sr[baic] increases baicalin's binding ability.

Interaction and Properties of the Synthesized Anionic Surfactant with CTAB: An Experimental and Theoretical Investigation

“…At the same time, the enthalpy and entropy change of micellization ( and ) are calculated by Equations 18 and : …”

Baicalin-aluminum complex on the regulation of IPEC-1 infected with enterotoxigenic Escherichia coli

“…Baicalin has also been used to treat diarrheic piglets by inhibiting bacterial adhesion and inflammatory responses due to ETEC infection [ 20 , 21 ]. With electron-rich carbonyl and carboxyl groups, baicalin can complex with metal ions to result in higher bioactivity [ 22 ]. It has previously been shown that baicalin-zinc and baicalin-copper can enhance the antioxidant capacity and nutrient absorption of pigs [ 23 , 24 ].…”

Comprehensive Biological, Photodegradation, Density Functional Theory, and Docking Exploration of Zn(II) and Mn(II) complexes of Piroxicam

“…[22] The binding capacity of the drug with the protein determines its pharmacodynamic properties like distribution, retention, excretion, and dosage frequency. [23][24][25] A large binding constant lessens free drug concentration in plasma and thus therapeutic effects would enhance. Drug toxicity dwindled with decreasing frequent use and increasing the binding with albumin protein.…”

“…Disulfide bonds organize the BSA single chain into nine loops for binding, while hydrogen bonds can easily be disrupted under the influence of chemical denaturants [22] . The binding capacity of the drug with the protein determines its pharmacodynamic properties like distribution, retention, excretion, and dosage frequency [23–25] . A large binding constant lessens free drug concentration in plasma and thus therapeutic effects would enhance.…”