Synthesis, Characterization, RP-HPLC Method Development and Validation for Qualitative Estimation of (4Z) 3 Methyl 1 (4 Nitrobenzoyl) 1H Pyrazole 4,5 Dione 4[(4fluorophenyl) Hydrazone]

Ashtekar, Harsha; Aggarwal, Natasha Naval; Raviraj, Chaitra; Rajesh, Gupta Dheeraj; Varghese, Nimmy

doi:10.5530/ijpi.13.2.041

Cited by 1 publication

(4 citation statements)

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“…Before starting the experiment, the system was also adjusted with the solvent system to obtain a smooth baseline. 10…”

Section: Methodsmentioning

confidence: 99%

“…H 2 O 2 is commonly used for oxidative breakdown and the samples were given at 3% H 2 O 2 treatment and kept at room temperature for 24 hours. 7 10 14…”

Section: Methodsmentioning

confidence: 99%

“…Before starting the experiment, the system was also adjusted with the solvent system to obtain a smooth baseline. 10 System Suitability and Method Validation Some of the key parameters like theoretical plates (TP; N), area under the curve (AUC), height equivalent to the theoretical plate (HETP), and percentage assay were all calculated using the chromatogram. 11 By using ICH methodologies, the technique's accuracy and limit of detection (LOD) were confirmed.…”

Section: Working Standardmentioning

confidence: 99%

“…H 2 O 2 is commonly used for oxidative breakdown and the samples were given at 3% H 2 O 2 treatment and kept at room temperature for 24 hours. 7,10,14 Forced degradation studies are essential in the development of analytical methods, specification setting, and formulation design within the quality by design (QbD) framework. Forced degradation is also known as stress testing and intentional degradation.…”

Section: Linearity and Beer's Rangementioning

confidence: 99%

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RP-HPLC-DAD Method Development and Validation for 1,3,4-Oxadiazole Derivative to Evaluate Its Forced Degradation Behavior and Stability

Deshpande,

BC,

Khot

et al. 2024

Journal of Health and Allied Sciences NU

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In the current study, a straightforward high-performance liquid chromatographic (HPLC) approach was developed and validated to identify a novel drug compound called 5-(4-bromophenyl)-N-(2-chloro-4-nitrophenyl)-1,3,4-oxadiazol-2-amine (A3). By putting the compound's solution under hydrolytic, oxidative, and photolytic stress, the method's capacity to detect stability was put to the test. A gradient mobile phase of acetonitrile, orthophosphoric acid, and methanol (90:051:05 v/v), at a flow rate of 1.00 mL/min, was used for the chromatographic separation on a C18 column (Promosil, 5μ, 4.60 250 mm), which was maintained at 40°C and a photodiode array detector was used for detection. At concentrations between 10 and 100.00 μg/mL, Beer's rule was observed. The recovery (99.25–100%, standard deviation [SD] 5%), intraday accuracy and precision (98.62–99.91%, relative standard deviation [RSD] 5%), interday accuracy and precision (96.25–99.91%, RSD 5%), and intermediate accuracy and precision (98.10–99.91%, RSD 5%) all indicated that the developed method was reliable, repeatable, reproducible, and robust. In cases of thermal and moisture deterioration, respectively, the compound's peak resolution and selectivity factors from the nearest resolving peak revealed specificity and selectivity. The selected synthesized compound barely broke down under oxidative and alkaline hydrolytic stress. However, the compound was resistant to photolysis in neutral and acidic environments. The results of this study demonstrate the sensitivity, specificity, and selectivity of the established approach for quality control, stability testing, and preformulation investigations.

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“…Before starting the experiment, the system was also adjusted with the solvent system to obtain a smooth baseline. 10…”

Section: Methodsmentioning

confidence: 99%

“…H 2 O 2 is commonly used for oxidative breakdown and the samples were given at 3% H 2 O 2 treatment and kept at room temperature for 24 hours. 7 10 14…”

Section: Methodsmentioning

confidence: 99%