The synthesis of the glycosidic prodrugs (1S)‐30a, (1S,10R)‐30b and (1S,10R)‐32 labelled with different fluorescence dyes at different positions at the aromatic A‐ring in 2 is described; the compounds are structurally based on the cytotoxic antibiotic duocarmycin SA. For binding, the amino compounds (1S)‐3a and (1S,10R)‐3b were treated with the commercially available succinimides of the dyes 5‐SFX (29) and D10162 (31), respectively.