Synthesis, Colloidal Properties and Cytotoxicity of Biopolymer Nanoparticles

Moorkoth, Dhanya; Nampoothiri, K. Madhavan

doi:10.1007/s12010-014-1172-z

Cited by 20 publications

(9 citation statements)

References 28 publications

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“…In addition, the label-free real-time electrical impedance showed neither reduced viability nor alterations to cell proliferation, in response to PLA-NPs. Altogether, these data are in accordance with previous studies showing that PLA-NP did not affect the viability of HEK293, CHO-K1, MCF-7, SK-BR-3 or retinal pigment epithelium cells measured through the analysis of formazan reaction products [ 6 , 7 , 43 , 46 ].…”

Section: Discussionsupporting

confidence: 92%

“…In fact, the Food and Drug Administration (FDA) have approved the use of biodegradable materials, including PLA-NP, in humans [ 73 ]. Previous studies have evaluated the cytotoxic potential of PLA-NP in different cell types, such as CHO-K1 [ 46 ], HEK293 [ 6 ], retinal pigment epithelium [ 7 ], and MCF-7 and SK-BR-3 breast cancer cell lineages [ 43 ]. These studies concluded that PLA-NP were non-cytotoxic; these conclusions were based on the assessment of cell viability through measurements of mitochondrial activity, while other cellular stress parameters were not considered.…”

Section: Introductionmentioning

confidence: 99%

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Poly-lactic acid nanoparticles (PLA-NP) promote physiological modifications in lung epithelial cells and are internalized by clathrin-coated pits and lipid rafts

et al. 2017

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BackgroundPoly-lactic acid nanoparticles (PLA-NP) are a type of polymeric NP, frequently used as nanomedicines, which have advantages over metallic NP such as the ability to maintain therapeutic drug levels for sustained periods of time. Despite PLA-NP being considered biocompatible, data concerning alterations in cellular physiology are scarce.MethodsWe conducted an extensive evaluation of PLA-NP biocompatibility in human lung epithelial A549 cells using high throughput screening and more complex methodologies. These included measurements of cytotoxicity, cell viability, immunomodulatory potential, and effects upon the cells’ proteome. We used non- and green-fluorescent PLA-NP with 63 and 66 nm diameters, respectively. Cells were exposed with concentrations of 2, 20, 100 and 200 µg/mL, for 24, 48 and 72 h, in most experiments. Moreover, possible endocytic mechanisms of internalization of PLA-NP were investigated, such as those involving caveolae, lipid rafts, macropinocytosis and clathrin-coated pits.ResultsCell viability and proliferation were not altered in response to PLA-NP. Multiplex analysis of secreted mediators revealed a low-level reduction of IL-12p70 and vascular epidermal growth factor (VEGF) in response to PLA-NP, while all other mediators assessed were unaffected. However, changes to the cells’ proteome were observed in response to PLA-NP, and, additionally, the cellular stress marker miR155 was found to reduce. In dual exposures of staurosporine (STS) with PLA-NP, PLA-NP enhanced susceptibility to STS-induced cell death. Finally, PLA-NP were rapidly internalized in association with clathrin-coated pits, and, to a lesser extent, with lipid rafts.ConclusionsThese data demonstrate that PLA-NP are internalized and, in general, tolerated by A549 cells, with no cytotoxicity and no secretion of pro-inflammatory mediators. However, PLA-NP exposure may induce modification of biological functions of A549 cells, which should be considered when designing drug delivery systems. Moreover, the pathways of PLA-NP internalization we detected could contribute to the improvement of selective uptake strategies.Electronic supplementary materialThe online version of this article (doi:10.1186/s12951-016-0238-1) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Poly-lactic acid nanoparticles (PLA-NP) promote physiological modifications in lung epithelial cells and are internalized by clathrin-coated pits and lipid rafts

et al. 2017

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“…The results also indicate that the PLLA microspheres produced by the oil in water method exhibit sensitivity to pH changes. This can be relevant for drug delivery applications with focus on the design and development of carriers for therapeutic and/or diagnostic purpose and also for in vivo applications and drug attachment [40]. The high zeta potential values obtained also indicate that the oil in water method does not result in a high adsorption of PVA in the surface of the microspheres.…”

Section: Physico-chemical and Thermal Propertiesmentioning

confidence: 85%

“…This method is also an important factor for determining the stability of PLLA and nanocomposite microspheres against aggregation, when dispersed in a liquid such as the cell culture media [40]. Generally, the stability of the microspheres in suspension improves with increasing absolute values of zeta potential, moreover higher stabilities are usual obtained for values higher than |À30| mV [41].…”

Section: Physico-chemical and Thermal Propertiesmentioning

confidence: 99%

Processing and size range separation of pristine and magnetic poly( l -lactic acid) based microspheres for biomedical applications

Correia

Sencadas

Ribeiro

et al. 2016

Journal of Colloid and Interface Science

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Biodegradable poly(l-lactic acid) (PLLA) and PLLA/CoFe2O4 magnetic microspheres with average sizes ranging between 0.16-3.9μm and 0.8-2.2μm, respectively, were obtained by an oil-in-water emulsion method using poly(vinyl alcohol) (PVA) solution as the emulsifier agent. The separation of the microspheres in different size ranges was then performed by centrifugation and the colloidal stability assessed at different pH values. Neat PLLA spheres are more stable in alkaline environments when compared to magnetic microspheres, both types being stable for pHs higher than 4, resulting in a colloidal suspension. On the other hand, in acidic environments the microspheres tend to form aggregates. The neat PLLA microspheres show a degree of crystallinity of 40% whereas the composite ones are nearly amorphous (17%). Finally, the biocompatibility was assessed by cell viability studies with MC3T3-E1 pre-osteoblast cells.

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“…In addition, 0.2‐ml aliquot from upper layers of liquid was collected at the same fixed time intervals. Transmittance values from collected samples were recorded at 550 nm and compared, to indicate NP kinetic stability .…”

Section: Methodsmentioning

confidence: 99%

Independent effect of polymeric nanoparticle zeta potential/surface charge, on their cytotoxicity and affinity to cells

et al. 2015

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Synthesis, Colloidal Properties and Cytotoxicity of Biopolymer Nanoparticles

Cited by 20 publications

References 28 publications

Poly-lactic acid nanoparticles (PLA-NP) promote physiological modifications in lung epithelial cells and are internalized by clathrin-coated pits and lipid rafts

Poly-lactic acid nanoparticles (PLA-NP) promote physiological modifications in lung epithelial cells and are internalized by clathrin-coated pits and lipid rafts

Processing and size range separation of pristine and magnetic poly( l -lactic acid) based microspheres for biomedical applications

Independent effect of polymeric nanoparticle zeta potential/surface charge, on their cytotoxicity and affinity to cells

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