Synthesis, Crystal Structure, DNA Binding, and Hydrolytic Cleavage Activity of a Manganese(II) Complex

Qian, Jing; Ma, Xiaofang; Xu, Haiyan; Tian, Jin‐Lei; Shang, Junlong; Zhang, Yuan; Shi, Yan

doi:10.1002/ejic.200901231

Cited by 15 publications

(5 citation statements)

References 48 publications

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“…The comparison with the monomeric complex shows, however, that under saturation conditions the kinetic benefit brought by the dimetallic complex is only two-fold. Similar complexes based on Mn(II), 50 Co(II) 51 and Gd(III) 52 have been also reported.…”

Section: Hydrolysis By ''Simple'' Mono and Bimetallic Complexessupporting

confidence: 78%

Progress in artificial metallonucleases

2012

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The development of synthetic agents able to hydrolytically cleave DNA with high efficiency and selectivity is still a fascinating challenge. Over the years, many examples have been reported reproducing part of the behaviour of the corresponding natural enzymes. Eventually, even the possibility to apply such systems to the manipulation of DNA of higher organisms has been demonstrated. However, efficiency of enzymes is still unrivalled. This feature article discusses the progress reported toward the realization of synthetic nucleases with particular attention to the comprehension of the reaction mechanisms and to the strategies that need to be addressed to obtain more efficient systems.

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Section: Hydrolysis By ''Simple'' Mono and Bimetallic Complexessupporting

confidence: 78%

Progress in artificial metallonucleases

2012

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“…Also, some synthetic iron and manganese complexes have exhibited great potential for anticancer drugs for their accumulation in tumor cells and stability, and some have even been used in clinical applications . Manganese and iron complexes constitute the most commonly studied artificial metallonucleases, showing efficient DNA cleavage ability, and their study will promote the development of synthetic drugs …”

Section: Introductionmentioning

confidence: 99%

DNA binding and nuclease activity of cationic iron(IV) and manganese(III) corrole complexes

Zhang

Wen

Wang

et al. 2014

Applied Organom Chemis

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Cationic meso(4-N-methylpyridyl)-based metallocorroles, μ-oxo iron corrole dimer (1b) and manganese corrole monomer (2b), were synthesized and characterized. The interactions of these two metal corrole complexes with CT-DNA were studied by UVvisible, fluorescence and circular dichroism spectroscopic methods, as well as by viscosity measurements. The results revealed that 1b interacts with CT-DNA in a difunctional binding mode, i.e. non-classical intercalation and outside groove binding with H-aggregation, while 2b can interact with CT-DNA via an outside groove binding mode only. The binding constants K b of 1b and 2b were 4.71 × 10 5 M À1 and 2.17 × 10 5 M À1 , respectively, indicating that 1b can bind more tightly to CT-DNA than 2b. Furthermore, both complexes may cleave the supercoiled plasmid DNA efficiently in the presence of hydrogen peroxide or tert-butyl hydroperoxide (TBHP), albeit 1b exhibited a little higher efficiency. The inhibitor tests suggested that singlet oxygen and high-valent (oxo)iron(VI) corrole or (oxo)manganese(V) corrole might be the active intermediates responsible for the oxidative DNA scission. Figure 2. UV-visible spectral changes of 1b in 5 mM Tris-HCl/50 mM NaCl buffer (pH 5), t = 0 (dashed line), 5, 10, 15 and 30 min.Figure 10. Absorption spectra of (a) 1b (20 μM) and (b) 2b (20 μM) in 50 mM Tris-HCl/18 mM NaCl buffer (pH 7.2) in the presence of TBHP or H 2 O 2 at different times. (a) [H 2 O 2 ] = [TBHP] = 10 μM; (b) [H 2 O 2 ] = [TBHP] = 20 mM.

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“…However, a comparatively very small number of manganese(II) complexes are investigated for DNA binding affinity and nuclease activity studies. [21][22][23] Sim-ilarly, zinc is also an important trace element and plays a significant role in maintaining overall human health throughout the whole lifespan. [24] It is the second most abundant trace metal found in the biological system and is found in many enzymes of living organisms.…”

Section: Introductionmentioning

confidence: 99%

“…Moreover, manganese complexes also exhibit important biological activities, such as DNA‐binding, albumin‐binding and antitumor activity. However, a comparatively very small number of manganese(II) complexes are investigated for DNA binding affinity and nuclease activity studies [21–23] . Similarly, zinc is also an important trace element and plays a significant role in maintaining overall human health throughout the whole lifespan [24] .…”

Section: Introductionmentioning

confidence: 99%

Cu(II), Mn(II) and Zn(II) Complexes of Anthracene‐Affixed Carboxylate‐Rich Tridentate Ligand: Synthesis, Structure, Spectroscopic Investigation and Their DNA Binding Profile

et al. 2022

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Anthracene-affixed carboxylate-rich tridentate ligand containing three new mononuclear complexes, [Cu(acpa)(H 2 O) 2 ]⋅H 2 O (1), [Mn(acpa)(H 2 O) 2 ] (2) and [Zn(acpa)(H 2 O) 2 ] (3) (H 2 acpa = 3-(anthracene-9-ylmethyl-carboxymethyl-amino)-propionic acid), have been synthesized and characterized by various analytical and spectral methods. In methanol, complexes 1-3 have been prepared by carrying out reaction of the ligand H 2 acpa with stoichiometric amounts of CuSO 4 ⋅ 5H 2 O, MnSO 4 ⋅H 2 O and ZnSO 4 ⋅ 7H 2 O respectively, in the presence of NaOH at ambient temperature. The X-ray crystal structure analysis reveals that complex 1 consists of a distorted square pyramidal copper center, coordinated to one acpa 2À ligand in a tridentate manner (N,O,O) and two exogenous water molecules. On the other hand, the molecular structures of complexes 2 and 3, optimized by DFT method indicate that their core arrangements around the metal centers are similar to that of 1. In solution, the coordination phenomena of Cu((II), Mn(II) and Zn(II) ions with the ligand H 2 acpa and the structural features of the complexes have been authenticated by UV-Vis and fluorescence titration experiments. The composition of the M II /acpa 2À complexes (M = Cu, Mn, Zn) in 1-3 has been observed to be 1 : 1, based on the UV-Vis/fluorescence titration results which are further supported by X-ray crystallography and DFT calculation. Complexes 1-3 are investigated for their binding affinity towards Calf Thymus (CT) DNA in solution at pH ∼ 7.5 by UV-Vis and fluorescence spectroscopic methods. From the electronic absorption spectral studies, it has been found that the DNA binding constants of 1-3 are (5.67 � 0.32) × 10 4 M À 1 , (2.35 � 0.26) × 10 4 M À 1 and (2.49 � 0.33) × 10 4 M À 1 , respectively, following the order 1 > 3 > 2. Measurements of viscosity of DNA in the absence and presence of the metal complexes suggest that all three complexes interact with DNA through partial intercalative mode.

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Synthesis, Crystal Structure, DNA Binding, and Hydrolytic Cleavage Activity of a Manganese(II) Complex

Cited by 15 publications

References 48 publications

Progress in artificial metallonucleases

Progress in artificial metallonucleases

DNA binding and nuclease activity of cationic iron(IV) and manganese(III) corrole complexes

Cu(II), Mn(II) and Zn(II) Complexes of Anthracene‐Affixed Carboxylate‐Rich Tridentate Ligand: Synthesis, Structure, Spectroscopic Investigation and Their DNA Binding Profile

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