Synthesis, double stranded DNA-binding and photocleavage studies of a functionalized ruthenium(II) complex with 7,7′-methylenedioxyphenyldipyrido[3,2-a:2′,3′-c]-phenazine

Tan, Lifeng; Liang, Xi-Ling; Liu, Xiaohua

doi:10.1016/j.jinorgbio.2008.12.010

Cited by 26 publications

(12 citation statements)

References 40 publications

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“…The observed melting temperatures in the presence of complexes 1 and 2 were 67.30 ± 0.50 and 66.99 ± 0.53°C, respectively. The increases in T m of the two Ru(II) complexes (the DT m is 6.00 and 5.69°C for 1 and 2) are comparable to that observed for Ru(II) complexes Tan et al 2009;Liu et al 2006) and lend strong support for intercalation into the helix of DNA. The experimental results also indicate that complex 1 exhibits larger DNA-binding affinity than complex 2 does.…”

Section: Thermal Denaturation Studiessupporting

confidence: 60%

“…The rich optical properties of these complexes facilitate assessments of their DNA binding capabilities as binding to DNA can be probed through changes in absorption and emission spectra. Many octahedral ruthenium(II) complexes have been proved to bind with DNA through intercalative mode (Erkkila et al 1999;Metcalfe and Thomas 2003;Zeglis et al 2007;Ghosh et al 2009;Liu et al 2009Liu et al , 2010Sun et al 2009;Tan et al 2009;Arockiasamy et al 2009;Yu et al 2009;Friedman et al 1990). These ruthenium complexes show interesting properties.…”

Section: Introductionmentioning

confidence: 99%

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2-(3,5-Dibromo-4-hydroxyphenyl)imidazo[4,5-f][1,10]phenanthrolinoruthenium(II) complexes: synthesis, characterization, cytotoxicity, apoptosis, DNA-binding and antioxidant activity

Liu

Liang

et al. 2010

Biometals

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A new ligand DBHIP and its two ruthenium(II) complexes [Ru(dmb)(2)(DBHIP)](ClO(4))(2) (1) and [Ru(dmp)(2)(DBHIP)](ClO(4))(2) (2) have been synthesized and characterized. The cytotoxicity of DBHIP and complexes 1 and 2 has been assessed by MTT assay. The apoptosis studies were carried out with acridine orange/ethidium bromide (AO/EB) staining methods. The binding behaviors of these complexes to calf thymus DNA (CT-DNA) were studied by absorption titration, viscosity measurements, thermal denaturation and photoactivated cleavage. The DNA-binding constants of complexes 1 and 2 were determined to be 8.64 +/- 0.16 x 10(4) (s = 1.34) and 2.79 +/- 0.21 x 10(4) (s = 2.17) M(-1). The results suggest that these complexes interact with DNA through intercalative mode. The studies on the mechanism of photocleavage demonstrate that superoxide anion radical (O(2)(*-)) and singlet oxygen ((1)O(2)) may play an important role in the DNA cleavage. The experiments on antioxidant activity show that these compounds also exhibit good antioxidant activity against hydroxyl radical (OH*).

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Section: Thermal Denaturation Studiessupporting

confidence: 60%

Section: Introductionmentioning

confidence: 99%

2-(3,5-Dibromo-4-hydroxyphenyl)imidazo[4,5-f][1,10]phenanthrolinoruthenium(II) complexes: synthesis, characterization, cytotoxicity, apoptosis, DNA-binding and antioxidant activity

Liu

Liang

et al. 2010

Biometals

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“…The observed melting temperatures in the presence of complexes 1 and 2 were 67.19 ± 0.5 and 68.92 ± 0.5°C, respectively. The increases in T m of the two Ru(II) complexes (the DT m is 5.19 and 6.92°C for 1 and 2) are comparable to those observed for Ru(II) complexes [8,31,32] and lend strong support for intercalation In vitro cytotoxicity assay…”

Section: Thermal Denaturation Studiessupporting

confidence: 59%

“…The interaction of transition metal polypyridyl complexes with DNA has been a topic of major bioinorganic interest in the past two decades and continues to receive much attention from researchers due to their importance in design and development of synthetic restriction enzymes, chemotherapeutic drugs and DNA footprinting agents [1][2][3][4][5][6][7][8]. Due to rich spectral characteristics and strong DNAbinding affinity, ruthenium(II) polypyridyl complexes have received particular attention.…”

Section: Introductionmentioning

confidence: 99%

DNA-binding, antioxidant activity and in vitro cytotoxicity induced by ruthenium(II) complexes containing polypyridyl ligands

Zeng

Liu

et al. 2010

Transition Met Chem

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Two new ruthenium(II) polypyridyl complexes, [Ru(dmp) 2 (maip)](ClO 4 ) 2 1 (maip = 2-(3-aminophenyl)-imizado[4,5-f][1,10]phenanthroline and [Ru(dmp) 2 (paip)]-(ClO 4 ) 2 2 (paip = 2-(4-aminophenyl)imidazo[4,5-f][1,10]-phenanthroline, dmp = 2, 9-dimethyl-1,10-phenanthroline) have been synthesized and characterized. The DNA-binding behaviors of complexes 1 and 2 were studied by viscosity measurements, thermal denaturation, and absorption titration. The results show that the two complexes intercalate between the base pairs of DNA. The DNA-binding constants K b for complexes 1 and 2 were determined to be 3.23 ± 0.16 9 10 4 M -1 (s = 0.97) and 4.34 ± 0.65 9 10 4 M -1 (s = 1.13). The cytotoxicity of these complexes has been evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The IC 50 values are 35.70, 41.04, 55.25 and 37.50 for complex 1 and 37.02, 103.08, 130.07 and 37.80 for complex 2 against BEL-7402, C-6, HepG-2 and MCF-7 cell lines, respectively. The antioxidant activity against hydroxyl radical (OH•) was also investigated.

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“…Binding of small molecules with DNA has been studied extensively since DNA is the material of inherence and controls the structure and function of cells [5]. In this respect ruthenium (II) complexes have attracted a great deal of attention due to their strong DNA-binding and potential anticancer [6][7][8][9][10][11][12]. In fact, the activity of many anticancer, antimalarial and antibacterical agents finds its origin in intercalative interactions with DNA [13].…”

Section: Introductionmentioning

confidence: 99%

Synthesis, DNA-binding, photocleavage, cytotoxicity and antioxidant activity of ruthenium (II) polypyridyl complexes

Liu¹,

Zeng²,

Huang³

et al. 2010

European Journal of Medicinal Chemistry

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Synthesis, double stranded DNA-binding and photocleavage studies of a functionalized ruthenium(II) complex with 7,7′-methylenedioxyphenyldipyrido[3,2-a:2′,3′-c]-phenazine

Cited by 26 publications

References 40 publications

2-(3,5-Dibromo-4-hydroxyphenyl)imidazo[4,5-f][1,10]phenanthrolinoruthenium(II) complexes: synthesis, characterization, cytotoxicity, apoptosis, DNA-binding and antioxidant activity

2-(3,5-Dibromo-4-hydroxyphenyl)imidazo[4,5-f][1,10]phenanthrolinoruthenium(II) complexes: synthesis, characterization, cytotoxicity, apoptosis, DNA-binding and antioxidant activity

DNA-binding, antioxidant activity and in vitro cytotoxicity induced by ruthenium(II) complexes containing polypyridyl ligands

Synthesis, DNA-binding, photocleavage, cytotoxicity and antioxidant activity of ruthenium (II) polypyridyl complexes

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