Synthesis of 28-membered macrocyclic polyammonium cations functionalized gold nanoparticles and their potential for sensing nucleotides

Bioconjugation of 32-macrocyclic polyammonium cations-functionalized gold nanoparticles with BSA

“…The 32-and 28-membered MCPAC-stabilised silver nanoparticles and their potential for sensing phosphates have been reported [10]. In another report [9], we described 28-membered MCPAC (28-MCPAC)-stabilized gold nanoparticles and their potential for sensing nucleotides. In this report, we extend our effort in finding suitable water-friendly stabilizers for gold nanoparticles and describe the synthesis of underivatized water dispersion 32-MCPAC-functionalized gold nanoparticles.…”

“…The aqueous Au-NPs were synthesized by the reduction of tetrachloroauric acid (HAuCl 4 ) with sodium borohydride (NaBH 4 ) in the presence of 32-MCPAC, following the previously reported method [9]. Typically, an aliquot of the aqueous solution of HAuCl 4 (2.50 mL, 5 mM stock solution, pH 2.40) was added to an aqueous solution of 32-MCPAC (50 mL, 1 mM stock solution, pH 4.20) with stirring at room temperature.…”

“…Consequently, the search for suitable stabilizers and the development of suitable facile methods are integral to Au-NP research. The dispersion of gold nanoparticles in a particular solvent (aqueous or nonaqueous) is strictly dependent on the type of stabilizers used [7][8][9][10]. Aqueous dispersions of nanoparticles are biocompatible, in particular underivatized nanoparticles because their properties are hardly dampened by the stabilizing agents.…”

“…Aqueous dispersions of nanoparticles are biocompatible, in particular underivatized nanoparticles because their properties are hardly dampened by the stabilizing agents. According to the literature, very few suitable stabilizing agents have thus far been found to be compatible with underivatized water-dispersed nanoparticles [7][8][9][10]. Of these stabilizing agents, citrate is the most commonly used water-friendly stabilizer [7].…”

“…Of these stabilizing agents, citrate is the most commonly used water-friendly stabilizer [7]. In this context, we recently discovered that macrocyclic polyammonium chlorides (MCPACs) could be used as stabilizers for underivatized water-dispersed nanoparticles [9,10]. We demonstrated that these MCPACs were capable of not only stabilizing the nanoparticles in water but also functionalizing the nanoparticles for sensing anions, since MCPACs are reputed anion receptors [11].…”