Synthesis of 3'- and 3',5'-modified oligonucleotides on functionalized silica Silochrom-2

Abstract

The preparation of 3'-mono-and 3', 5'-bis-labeled oligonucleotides on a new fluorescein-functionalized polymer support based on Silochrom-2 microspherical silica is described. Oligonucleotide (dT) 15 containing a dye residue at 3'-end was synthesized on this support by solid-phase phosphoramidite method. Post-synthetic 5'-modification of 3'-conjugate allowed obtaining double dye-labeled oligomer.