2014
DOI: 10.1007/s00216-014-8224-7
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Synthesis of [77Se]-methylselenocysteine when preparing sauerkraut in the presence of [77Se]-selenite. Metabolic transformation of [77Se]-methylselenocysteine in Wistar rats determined by LC–IDA–ICP–MS

Abstract: The use of enriched Se isotopes as tracers has provided important information on Se metabolism. However, selenium isotopes are expensive and difficult to obtain. A simple and cheap strategy based on the production of [(77)Se]-methylselenocysteine ([(77)Se]-MeSeCys) when preparing sauerkraut in the presence of [(77)Se]-selenite was developed. The resulting [(77)Se]-MeSeCys was used for evaluating the metabolic transformation of MeSeCys in Wistar rats, by feeding them with an AIN-93 M diet containing 20 % sauerk… Show more

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Cited by 6 publications
(2 citation statements)
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“…After a certain period of sonication and shaking for 20 h, at 37 °C and 121 r min −1 , the extract was centrifuged at 8000 g for 20 min. The liquid supernatant was collected and filtered consecutively through a 0.45‐μm nylon membrane (Sánchez‐Martínez et al ., ). Se species were measured immediately after extraction.…”
Section: Methodsmentioning
confidence: 97%
“…After a certain period of sonication and shaking for 20 h, at 37 °C and 121 r min −1 , the extract was centrifuged at 8000 g for 20 min. The liquid supernatant was collected and filtered consecutively through a 0.45‐μm nylon membrane (Sánchez‐Martínez et al ., ). Se species were measured immediately after extraction.…”
Section: Methodsmentioning
confidence: 97%
“…The metabolism of MeSeCys in rats by using Me 77 SeCys as tracer was evaluated by Sanchez-Martinez et al 187 For this purpose Wistar rats were fed with a diet containing Me 77 SeCysenriched sauerkraut. Analysis of major organ extracts by HPLC-IDA-ICP-MS showed a prominent increase (more than a 100% of Se level enhancement) of Se in kidney and heart, whereas in liver the Se concentration only increased up to 20% and remained constant in brain and testicles.…”
Section: àmentioning
confidence: 99%