Synthesis of a cortisol-biotin conjugate and evaluation as a tracer in an immunoassay for salivary cortisol measurement

Dressendörfer, Regina A.; Kirschbaum, Clemens; Rohde, Wolfgang; Stahl, F; Strasburger, Christian J.

doi:10.1016/0960-0760(92)90294-s

Cited by 824 publications

(437 citation statements)

References 14 publications

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“…Saliva-derived cortisol was analyzed using a time-resolved fluorescence immunoassay (Dressendorfer et al, 1992) and the area under the curve (AUC; mg/dl/ min) was calculated for each subject and each scanning Stress-induced dopamine release in schizotypy A Soliman et al session (Pruessner et al, 2003). Heart rate, electromyography (EMG), and skin conductance were measured throughout each PET session by electrodes placed on the left arm and chest (F1000 system, Focused Technology, Ridgecrest, CA 93556).…”

Section: Psychological and Physiological Measuresmentioning

confidence: 99%

Stress-Induced Dopamine Release in Humans at Risk of Psychosis: a [11C]Raclopride PET Study

Soliman

O’Driscoll

Pruessner

et al. 2007

Neuropsychopharmacol

133

109

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Drugs that increase dopamine levels in the brain can cause psychotic symptoms in healthy individuals and worsen them in schizophrenic patients. Psychological stress also increases dopamine release and is thought to play a role in susceptibility to psychotic illness. We hypothesized that healthy individuals at elevated risk of developing psychosis would show greater striatal dopamine release than controls in response to stress. Using positron emission tomography and [ 11 C]raclopride, we measured changes in synaptic dopamine concentrations in 10 controls and 16 psychometric schizotypes; 9 with perceptual aberrations (PerAb, ie positive schizotypy) and 7 with physical anhedonia (PhysAn, ie negative schizotypy). [ 11 C]Raclopride binding potential was measured during a psychological stress task and a sensory-motor control. All three groups showed significant increases in self-reported stress and cortisol levels between the stress and control conditions. However, only the PhysAn group showed significant stress-induced dopamine release. Dopamine release in the entire sample was significantly negatively correlated with smooth pursuit gain, an endophenotype linked to frontal lobe function. Our findings suggest the presence of abnormalities in the dopamine response to stress in negative symptom schizotypy, and provide indirect evidence of a link to frontal function.

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Section: Psychological and Physiological Measuresmentioning

confidence: 99%

Stress-Induced Dopamine Release in Humans at Risk of Psychosis: a [11C]Raclopride PET Study

Soliman

O’Driscoll

Pruessner

et al. 2007

Neuropsychopharmacol

133

109

View full text Add to dashboard Cite

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“…The free cortisol concentration in saliva was measured using a time-resolved immunoassay with fluorescence detection, as described previously (Dressendoerfer et al, 1992). Intra and inter assay variability was less than 10 and 12 percent, respectively.…”

Section: Cortisol Assaymentioning

confidence: 99%

Decreased cortisol awakening response after early loss experience

Meinlschmidt

Heim

2005

Psychoneuroendocrinology

141

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SummaryEarly loss experience (ELE) due to death or separation is a major risk factor for the development of several psychiatric and physical disorders in adulthood. Few studies have focused on the effects of ELE on neuroendocrine systems, which might mediate this risk in part. The goal of this study was to evaluate salivary cortisol responses to awakening in individuals with and without ELE. A total of 95 healthy college students (29 men, 66 women) completed a questionnaire on ELE and were instructed to collect saliva immediately after awakening and 30 minutes later. Fifty-five of the 95 subjects reported having experienced the separation or divorce of their parents and/or the death of a close relative before the age of 14 years. Subjects with such ELE exhibited decreased salivary cortisol responses to awakening compared to subjects without ELE (net increase: 4.78 nmol/l versus 9.83 nmol/l; t 93 =2.88, p=.005). The effect was most pronounced in individuals who experienced multiple types of ELE, while there were no sex differences. In conclusion, ELE appears to be associated with decreased salivary cortisol responses to awakening. Low cortisol awakening responses are believed to reflect altered dynamics of the hypothalamic-pituitary-adrenal (HPA) axis, possibly conferring risk for certain stress-related disorders.[200 words]

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“…Cortisol levels are robust to environmental conditions associated with the shipping process (Clements and Parker, 1998). Cortisol levels were determined in duplicate using a competitive solid phase time-resolved fluorescence immunoassay with fluorometric end point detection (Dressendorfer et al, 1992). The intra-assay coefficients of variation were less than 11%.…”

mentioning

confidence: 99%

Sampling compliance for cortisol upon awakening in children and adolescents

Rotenberg

McGrath

2014

Psychoneuroendocrinology

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SummaryCompliance with awakening salivary sampling is important for precise measurement of the diurnal cortisol profile. During childhood and adolescence, developmental factors influence sampling upon awakening (awake 0 ) due to school routine, sleep/wake patterns, and age related cortisol changes. In the present study, children and adolescents' sampling compliance of awakening cortisol was evaluated using accelerometry. Children and adolescents (N = 201; 45.3% female; 8-18 years; M age = 12.68 years, SD = 2.03) participating in the Healthy Heart Project collected saliva samples, wore a tri-axle accelerometer, and completed demographic questionnaires. Intraclass correlations derived to examine awake 0 sampling compliance indicated children and adolescents were highly compliant (ICC = .98). In children, a delay in awake 0 sampling was associated with a steeper diurnal slope (β = −.23, p = .037) and greater awake 0 cortisol (β = .24, p = .024); this was not observed in adolescents. In summary, children and adolescents are compliant with awakening salivary sampling. Sampling delay, particularly in children, and time of awakening influenced measures of the diurnal cortisol profile. These findings inform future studies assessing the diurnal cortisol profile in children and adolescents.

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Synthesis of a cortisol-biotin conjugate and evaluation as a tracer in an immunoassay for salivary cortisol measurement

Cited by 824 publications

References 14 publications

Stress-Induced Dopamine Release in Humans at Risk of Psychosis: a [11C]Raclopride PET Study

Stress-Induced Dopamine Release in Humans at Risk of Psychosis: a [11C]Raclopride PET Study

Decreased cortisol awakening response after early loss experience

Sampling compliance for cortisol upon awakening in children and adolescents

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