Synthesis of a Di-Mycoloyl Tri-Arabinofuranosyl Glycerol Fragment of the Mycobacterial Cell Wall, Based on Synthetic Mycolic Acids

Ali, Omar T.; Mohammed, Mohsin O.; Dulayymi, Juma’a R. Al; Baird, Mark S.

doi:10.3390/molecules24193596

Cited by 3 publications

(1 citation statement)

References 32 publications

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“…Their structures are presented in Supplementary Figure S1 . Antigen MOD171 is based on a cell-wall-bound mycolic acid fragment, a triarabinose dimycolate, incorporating a major α-mycolic acid present in many mycobacteria including MAC [ 33 ]. ZAM295 is a trehalose dimycolate of a diene-mycolic acid, a sub-group commonly present in NTM [ 34 ].…”

Section: Methodsmentioning

confidence: 99%

An ELISA Using Synthetic Mycolic Acid-Based Antigens with DIVA Potential for Diagnosing Johne’s Disease in Cattle

Mason,

Holder,

Robinson

et al. 2024

Animals

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The problem: Ante-mortem diagnosis of Johne’s disease, caused by Mycobacterium avium subsp. paratuberculosis (MAP), is normally achieved through faecal culture, PCR, or serological tests, but agreement as to which samples are positive for Johne’s disease is often poor and sensitivities are low, particularly in early-stage infections. The potential solution: Mycobacterial cells contain very complex characteristic mixtures of mycolic acid derivatives that elicit antibodies during infection; this has been used to detect infections in humans. Here, we explore its application in providing an assay differentiating infected from vaccinated animals (DIVA assay) for Johne’s disease in cattle. Method: Antibody responses to different classes of mycolic acid derivatives were measured using ELISA for serum from cattle positive for MAP by both faecal PCR and commercial serum ELISA, or just by PCR, and from animals from herds with no history of Johne’s disease, bovine tuberculosis reactors, BCG-vaccinated, BCG-vaccinated and M. bovis-infected, and Gudair-vaccinated animals. Results: The best-performing antigens, ZAM295 and ST123—the latter a molecule present in the cells of MAP but not of Mycobacterium bovis—achieved a sensitivity of 75% and 62.5%, respectively, for serum from animals positive by both faecal PCR and a commercial MAP serum ELISA, at a specificity of 94% compared to 80 no-history negatives. Combining the results of separate assays with two antigens (ST123 and JRRR121) increased the sensitivity/specificity to 75/97.5%. At the same cut-offs, animals vaccinated with Gudair or BCG vaccines and bTB reactors showed a similar specificity. The specificity in BCG-vaccinated but M. bovis-infected animals dropped to 85%. Combining the results of two antigens gave a sensitivity/specificity of 37.5/97.5% for the full set of 80 PCR-positive samples, detecting 30 positives compared 16 for IDEXX. Conclusion: Serum ELISA using synthetic lipids distinguishes effectively between MAP-negative cattle samples and those positive by both PCR and a commercial MAP serodiagnostic, without interference by Gudair or BCG vaccination. It identified almost twice as many PCR positives as the commercial serodiagnostic, offering the possibility of earlier detection of infection.

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Section: Methodsmentioning

confidence: 99%

An ELISA Using Synthetic Mycolic Acid-Based Antigens with DIVA Potential for Diagnosing Johne’s Disease in Cattle

Mason,

Holder,

Robinson

et al. 2024

Animals

Self Cite

View full text Add to dashboard Cite

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Early antibody responses to lipid antigens in red deer infected with Mycobacterium bovis

Mason,

Risalde,

Gortázar

et al. 2024

Veterinary Microbiology

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New spiro-heterocyclic coumarin derivatives as antibacterial agents: design, synthesis and molecular docking

Al-burgus,

Ali,

Al-abbasy

2024

Chim.Tech.Acta

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Coumarin derivatives were synthesized herein from 3-acetyl coumarin and 4-(pyrimidin-2-yldiazenyl) antipyrine, leading to the azo chalcone intermediate compound. The final spiro-heterocyclic coumarins were produced through the cyclization of the azo-chalcone with thiourea, guanidine hydrochloride, benzene-1,2-diamine, 2-aminophenol, and hydroxylamine hydrochloride, respectively. The obtained target compounds were purified by column chromatography and characterized by FT-IR, 1H NMR, 13C-NMR and elemental analysis. The antibacterial activity of the synthesized compounds was evaluated in vitro against Gram-negative and Gram-positive bacteria. One of the compounds showed significant antibacterial activity. Furthermore, the docking study of this compound with DNA gyrase for E. coli and S. aureus bacterial strains was investigated, which revealed vital interactions and binding.

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Synthesis of a Di-Mycoloyl Tri-Arabinofuranosyl Glycerol Fragment of the Mycobacterial Cell Wall, Based on Synthetic Mycolic Acids

Cited by 3 publications

References 32 publications

An ELISA Using Synthetic Mycolic Acid-Based Antigens with DIVA Potential for Diagnosing Johne’s Disease in Cattle

An ELISA Using Synthetic Mycolic Acid-Based Antigens with DIVA Potential for Diagnosing Johne’s Disease in Cattle

Early antibody responses to lipid antigens in red deer infected with Mycobacterium bovis

New spiro-heterocyclic coumarin derivatives as antibacterial agents: design, synthesis and molecular docking

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