Synthesis of a N′-Alkylamine Anticoagulant Active Low-Molecular-Mass Heparin for Radioactive and Fluorescent Labeling

Semisynthesis and Analysis of Lipophilically Modified Unfractionated and Low Molecular Mass Heparins

“…11 Nuclear mag netic resonance (NMR) studies reveal the molecular structure of the molecules and give evidence about the position and the amount of conjugate bound. 12 Spectro scopic data also prove the binding of conjugates with chromophores and fluorophores to LMMH. 13 The antico agulant potential of the modified heparins is evaluated in vitro by anti-Factor Xa and anti-Factor IIa assays and the inhibition of the formation of the thrombin generation inhibition.…”

Determination of heparin‐induced IgG antibody in heparin‐induced thrombocytopenia type II

“…Danaparoid sodium was supplied by N. V. Organon (Oss, The Netherlands) with an activity of 10 IU mg À1 . Unfractionated heparin was labelled with uorescein-5-isothiocyanate (FITC) as described previously [11]. The parent compound and the conjugate both had a mean molecular weight of 12 000 dalton [12] and activities of 160 IU mg À1 .…”

“…After switching heparin treatment to other anticoagulants, the normalization of HI-Abs is of clinical relevance, but its quantitative determination remains dif®cult. In this paper we report a sensitive and speci®c assay for the relative quantitative detection of HI-IgG with¯uorescein-labelled heparin (FITC-heparin) [11], including an analysis on the cross-reactivities of HI-IgG with LMWH and danaparoid.…”

Neoglycoconjugates

“…Oligosaccharides containing this active aldehydo group at the reducing terminus can be used for conjugation to proteins or amino compounds by reductive amination. This was the approach used to prepare low-mass heparin derivatives possessing anticoagulant activities (Malsch et al, 1994).…”