Synthesis of a Novel 4,4’-[1,4-phenylenebis(1,3,4-thiadiazole-5,2-diyl)] bis(azaneylylidene) bis(methaneylylidene) diphenol and Determination of Its pharmacological and antimicrobial Activities

Saeed, Ban M. S.; Al-Jadaan, Shaker A. N.; Abbas, Basil A.

doi:10.1088/1742-6596/1279/1/012037

Cited by 3 publications

(1 citation statement)

References 13 publications

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“…Several studies were done on Bacillus cereusin the similar area of study 33,34,35,36,37 . Bacteriocin production rarely investigated which considered as effective food preservatives.…”

Section: Discussionmentioning

confidence: 99%

Bacteriocin Production in Bacillus cereus Food Isolates with Molecular Detection of cerA gene

2020

IJFMT

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Sixty-three Bacillus cereus strains isolated from food samples. All strains were subjected to DNA sequencing for 16S rRNA for identification. 15 strains were registered at GenBank of NCBI and given new accession numbers. 41.26% of the isolates showed bacteriocinogenicproduction activity against four bacterial speciesviz,Bacillus cereus, Staphylococcus aureus, Escherichia coli and Salmonella sp. Presence of cerA gene coding for cericidine(a type of bacteriocin) was detected in 7.69% of the isolates that produced bacteriocin. Bacterionwas precipitated by ammonium sulphate and purified by dialysis. Antimicrobial activity of precipitated bacteriocin against the four types of bacteria showed the effect on Bacillus cereus and Staphylococcus aureus growth but not on Escherichia coli and Salmonella spp.The produced bacteriocin has a molecular weight ranging from 47-54 KDa.Estimation of the concentration and physical and chemical properties of bacteriocin were also investigated.

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“…Several studies were done on Bacillus cereusin the similar area of study 33,34,35,36,37 . Bacteriocin production rarely investigated which considered as effective food preservatives.…”

Section: Discussionmentioning

confidence: 99%

Bacteriocin Production in Bacillus cereus Food Isolates with Molecular Detection of cerA gene

2020

IJFMT

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Detection of Bacillus cereus genes responsible for diarrheal and emetic toxins

Saeed

Abbas

Al-Jadaan³

2021

J. Phys.: Conf. Ser.

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Bacillus cereus isolated from different food sources. The diarrheal toxin genes such as cytK, hemolytic enterotoxin (hblA, hblC and hblD), non-hemolytic enterotoxin (nheA, nheB and nheC), bceT and entFM in addition to emetic toxin gene were detected by PCR. The cyt K gene was observed in 94.87 % of the isolates. entFM and emetic toxin gene were found very rare in all food samples at the percentage 2.56% and 7.69% respectively. Uncooked rice which has a highest number of bacterial isolation, also showed relatively high percentage of the cytK and bceT genes (90%). These two genes present in 100% of Bacillus cereus isolates in most food samples. Bacteria isolated from burger meat contain all investigated genes.

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Synthesis, Characterization of a Novel 1,1’-[1,4-phenylenebis(1,3,4-thiadiazol-5,2-diyl)] bis (3-chloro-4-(4-hydroxyphenyl) azetidin-2-one and evaluation its Biological activities

Saeed

Al-Jadaan

Abbas

2020

IOP Conf. Ser.: Mater. Sci. Eng.

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In current study, synthesis of a novel azetidin-2-one compound, i.e 1,1’-[1,4-phenylenebis(1,3,4-thiadiazol-5,2-diyl)] bis (3-chloro-4-(4-hydroxyphenyl) azetidin-2-one from reaction of 0.002mole of 4,4’-[1,4-phenylene bis(1,3,4-thiadiazole-5,2-diyl)] bis (azaneylylidene) bis (methaneylylidene) diphenol with 0.004mole of 2-chloroacetylchloride in dioxane as a solvent in the presence of trimethylamine. The biological activity of new azetidin-2-one compound was evaluated at 100 mg/ml against four types of bacteria i.e. Bacillus cereus, Staphylococcus aureus, Escherichia coli and Salmonella sp. and its minimum inhibitory concentration (MIC) was 2, 2, 3, 3 mg/ml for Bacillus cereus, Staphylococcus aureus, Escherichia coli and Salmonella sp. respectively. Median lethal dose (LD50) and cytotoxicity activity were also investigated. The LD50 for this compound is 1.4 gm/kg bw. The results showed that this compound did not affect the red blood cell until the concentration reach 25 mg/ml or above. The new compound was characterized by spectral data, i.e., FT-IR, 1H-NMR, 13C-NMR and elemental analysis which confirmed its proposed structure.

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Synthesis of a Novel 4,4’-[1,4-phenylenebis(1,3,4-thiadiazole-5,2-diyl)] bis(azaneylylidene) bis(methaneylylidene) diphenol and Determination of Its pharmacological and antimicrobial Activities

Cited by 3 publications

References 13 publications

Bacteriocin Production in Bacillus cereus Food Isolates with Molecular Detection of cerA gene

Bacteriocin Production in Bacillus cereus Food Isolates with Molecular Detection of cerA gene

Detection of Bacillus cereus genes responsible for diarrheal and emetic toxins

Synthesis, Characterization of a Novel 1,1’-[1,4-phenylenebis(1,3,4-thiadiazol-5,2-diyl)] bis (3-chloro-4-(4-hydroxyphenyl) azetidin-2-one and evaluation its Biological activities

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