Synthesis Of Alkoxy-Isoflavones As Potential α-Glucosidase Inhibitors

Aguila-Muñoz, Dolores G.; Cervantes-Espinoza, Elizabeth; Escalante, Carlos H.; Gutiérrez, Rsuini U.; Cruz-López, María C.; Jiménez-Montejo, Fabiola E.; Villa‐Ruano, Nemesio; Gómez-García, Omar; Tamariz, Joaquı́n; Mendieta‐Moctezuma, Aarón

doi:10.21203/rs.3.rs-1351698/v1

Cited by 1 publication

(1 citation statement)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results are illustrated in 2D and 3D (Fig. 3(a) and 3(b)), revealing that 5c recognized some of the key amino acid residues in the catalytic pocket, such Arg213, Asp215, Glu277, His351, Asp352, and Arg442 [22,34]. Regarding the binding energy of the compound 5c with the enzymes, has better binding energy values (ΔG) than the reference drug (6) (Table 2).…”

Section: Molecular Docking Analysismentioning

confidence: 91%

Alpha-glucosidase and Alpha-amylase Inhibitors Derived from Naturally Occurring Prenylated Isoflavones

Hernández,

Cruz,

Gómez

et al. 2024

J. Mex. Chem. Soc.

View full text Add to dashboard Cite

A series of prenylated isoflavones were synthesized to evaluate their inhibitory effect against α-glucosidase and α-amylase enzymes, analyzing the bioisosteric effect of the linear or cyclized prenyl moiety in these benzopyran derivatives. Compound 5a exhibited higher α-glucosidase inhibition (IC50 = 60.5 µM) and lower α-amylase inhibition (IC50 = 85.0 µM) compared to acarbose (IC50 = 527.5 µM for α-glucosidase and 20.1 µM for α-amylase). In contrast, prenylated isoflavone 5c showed higher inhibition in both enzymes (IC50 = 17.6 µM for α-glucosidase and 21.2 µM for α-amylase). This suggests that the attachment of a prenyl moiety to the 7-hydroxy group of isoflavone provides higher inhibition in the enzymes α-glucosidase and α-amylase. Docking studies of compounds 5a and 5c displayed key interactions towards both enzymes. The type of inhibition for 5c was analyzed, where the results indicate a competitive inhibition of both α-glucosidase and α-amylase. Finally, ADMET studies support that compounds 5a and 5c are candidates for the design of novel isoflavones derivatives with antidiabetic potential. Resumen. Una serie de isoflavonas preniladas se sintetizaron para evaluar su efecto inhibidor sobre las enzimas α-glucosidasa y α-amilasa, analizando el efecto bioisotérico del fragmento prenilo tipo lineal o ciclado en estos benzopiranos derivados. El compuesto 5a exhibió una inhibición alta de α-glucosidasa (CI50 = 60.5 µM) y una inhibición más baja de α-amilasa (CI50 = 85.0 µM, respectivamente) en comparación con acarbosa (CI50 = 527.5 y 20.1 µM). La isoflavona prenilada 5c mostró mayor inhibición en ambas enzimas (CI50 = 17.7 µM para α-glucosidasa y 21.2 µM para α-amilasa). Esto sugiere que la unión del fragmento prenilo al hidroxilo de la posición 7 de la isoflavona ocasiona una mayor inhibición en las enzimas α-glucosidasa y α-amilasa. Los compuestos 5a y 5c mostraron interacciones clave hacia el sitio activo de ambas enzimas, de acuerdo con los cálculos de acoplamiento. Se analizó el tipo de inhibición para 5c, donde los resultados indican una inhibición competitiva tanto de α-glucosidasa como de α-amilasa. Finalmente, los estudios ADMET respaldan que los compuestos 5a and 5c son candidatos para el diseño de nuevos derivados de isoflavonas con potencial antidiabético.

show abstract

Section: Molecular Docking Analysismentioning

confidence: 91%