Synthesis of alkyne-tagged and biotin-tagged Sortin1 as novel photoaffinity probes

In Planta Labeling Using a Clickable ER-Disrupting Probe Suggests a Role for Oleosins in Arabidopsis Seedling ER Integrity

“…On the basis of our observations that ER vesiculation is most prominent in young seedlings, we conducted labeling experiments using 3- to 4-day old dark-grown Arabidopsis seedlings germinated and grown in the presence of either 5 μM 2 (active), 3 (inactive), or eroonazole (which served as unclickable background control), adopting a commonly used PAL and click chemistry approach previously employed in plants for activity-based protein profiling and the small molecule inhibitor sortin1. , Briefly, probe-treated seedlings were exposed to 254 nm light for 30 min, and cell lysates were prepared and separated into microsomal and soluble fractions. The photolabeled lysates were then subjected to click reactions with both cleavable (BT3) and noncleavable (BT2) biotin picolyl azide tags (Data File S1).…”

Synthesis and application of new rhodamine 6G-derivative fluorescent probe molecules

“…The limit content of elemental mercury in drinking water in China is 1μg/L, and in the field of drinking water purification, the design of rhodamine fluorescent probe can meet the standard of detection limit [6]. At present, there have been many reports about the mercury ion fluorescent probe designed on the principle of the "off-open" mechanism, however, many of the synthesized probes are known to have shortcomings such as low sensitivity, poor selectivity, and limited application range in the detection process [7][8][9][10][11].…”