Synthesis of Amphiphilic Derivatives of Rose Bengal and Their Tumor Accumulation

Sugita, Nami; Kawabata, K.; Sasaki, Kenji; Sakata, Isao; Umemura, Shin Ichiro

doi:10.1021/bc060189p

Cited by 45 publications

(41 citation statements)

References 27 publications

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“…It is known that the effects of SDT are partly dependent on the concentration of sono-sensitizers accumulated in the tumor cells [25,26] , the accumulations of sono-sensitizers have been reported in a variety of tumors, this is important because these time courses can be different depending not only on the sensitizer but also on animal species, and the tumor cell lines [27] . Because the intracellular Hp accumulation has not been carefully investigated, we measured the intracellular Hp concentration after adding to S180 cells in vitro, SDT would be most effective if cells exposed to ultrasound when the Hp concentration was relatively higher.…”

Section: Discussionmentioning

confidence: 99%

Enhancement of Ultrasonically Induced Cytotoxic Effect by Hematoporphyrin in vitro

Wang

Wang²,

Liu³

et al. 2008

Chemotherapy

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It was the aim of this study to investigate the ultrasonically induced cytotoxic effect of hematoporphyrin (Hp) on isolated sarcoma 180 (S180) cells and to explore the potential biological mechanism of this action. S180 tumor cells suspended in air-saturated phosphate-buffered saline (pH 7.2) were exposed to ultrasound at 1.6 MHz in a standing wave mode for up to 90 s with and without 100 µg/ml Hp. The intracellular Hp concentration was evaluated to determine the optimum timing of ultrasound exposure after its administration with a fluorescence spectrophotometer based on the standard curve. Cell viability was determined by the trypan blue exclusion test. The morphological changes of S180 cells induced by ultrasonic irradiation were evaluated by scanning electron microscope and transmission electron microscope observation. The participation of lipid peroxidation products in the cell damage process was investigated by the malon aldehyde content test. Our experiments suggested that an incubation time of 45 min after the addition of 100 µg/ml Hp was to be chosen as the best time for ultrasound exposure in vitro. The rate of ultrasonically induced cell damage was enhanced by the increase in ultrasound intensity and exposure time in the presence and absence of Hp. At an ultrasound intensity not less than 3 W/cm² and an irradiation time not less than 60 s, a significant synergistic effect of ultrasound combined with Hp was observed, while no cell damage was observed with 100 µg/ml Hp alone. The malon aldehyde content test showed that the lipid peroxidation level significantly increased after sonodynamic therapy treatment. Scanning electron microscope and transmission electron microscope observation indicated that the changes in cell ultra-structure such as cell membrane destruction, mitochondria swelling and chromatin condensation are important factors that induced cell damage in S180 tumor cells. The results indicate that the biological mechanism might be involved in mediating the killing effect on S180 cells, and the ultra-structural changes may account for cell destruction induced by sonodynamic therapy treatment in our experiment mode.

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Section: Discussionmentioning

confidence: 99%

Enhancement of Ultrasonically Induced Cytotoxic Effect by Hematoporphyrin in vitro

Wang

Wang²,

Liu³

et al. 2008

Chemotherapy

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“…These accumulate at higher concentrations in the tumor tissue than rose bengal does. 61 The ability of US to activate the sonosensitizers and the ability of sonosensitizers to accumulate selectively into tumors suggest that SDT could be a useful clinical treatment for cancers located deep in the human body.…”

Section: Us and Sonosensitizersmentioning

confidence: 99%

Therapeutic potential of low-intensity ultrasound (part 2): biomolecular effects, sonotransfection, and sonopermeabilization

et al. 2008

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Part one of this review focused on the thermal and mechanical effects of low-intensity ultrasound (US). In this second and final part of the review, we will focus on and discuss various aspects of low-intensity US, with emphasis on the biomolecular effects, US-mediated gene transfection (sonotransfection), and US-mediated permeabilization (sonopermeabilization). Sonotransfection of different cell lines in vitro and target tissues in vivo have been reported. Optimization experiments have been done and different mechanisms investigated. It has also been found that several genes can be up-regulated or down-regulated by sonication. As to the potential therapeutic applications, systemic or local sonotransfection might also be a safe and effective gene therapy method in effecting the cure of local and systemic disorders. Gene regulation of target cells may be utilized in modifying cellular response to a treatment, such as increasing the sensitivity of diseased cells while making normal cells resistant to the side effects of a treatment. Advances in sonodynamic therapy and drug sonopermeabilization also offer an ever-increasing array of therapeutic options for low-intensity US.

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“…,64 Such a substitution Scheme 3. Protonation state of fluorescein derivative tautomers at various pH.…”

mentioning

confidence: 99%

Rose Bengal analogs and vesicular glutamate transporters (VGLUTs)

Pietrancosta

Kessler

Favre-Besse

et al. 2010

Bioorganic & Medicinal Chemistry

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Synthesis of Amphiphilic Derivatives of Rose Bengal and Their Tumor Accumulation

Cited by 45 publications

References 27 publications

Enhancement of Ultrasonically Induced Cytotoxic Effect by Hematoporphyrin in vitro

Enhancement of Ultrasonically Induced Cytotoxic Effect by Hematoporphyrin in vitro

Therapeutic potential of low-intensity ultrasound (part 2): biomolecular effects, sonotransfection, and sonopermeabilization

Rose Bengal analogs and vesicular glutamate transporters (VGLUTs)

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