Synthesis of Anionic Nanogels for Selective and Efficient Enzyme Encapsulation

Ni, Jiaying; Wan, Yuting; Cai, Ying; Ding, Peng; Stuart, Martien A. Cohen; Wang, Junyou

doi:10.1021/acs.langmuir.1c03325

Cited by 13 publications

(12 citation statements)

References 53 publications

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“…As shown in Fig. 1A, the core-shell polyelectrolyte NPs were synthesized on the basis of our recently developed strategy involving cooperative electrostatic assembly and polymerization ( 27 , 28 ). We first modified a PEG block [weight-average molecular weight ( M w ) = 5000; fig.…”

Section: Resultsmentioning

confidence: 99%

Rational polyelectrolyte nanoparticles endow preosteoclast-targeted siRNA transfection for anabolic therapy of osteoporosis

et al. 2023

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Targeted transfection of siRNA to preosteoclasts features the potential of anti-osteoporosis, yet challenge arises from the development of satisfied delivery vehicles. Here, we design a rational core-shell nanoparticle (NP) composed of cationic and responsive core for controlled load and release of small interfering RNA (siRNA) and compatible polyethylene glycol shell modified with alendronate for enhanced circulation and bone-targeted delivery of siRNA. The designed NPs perform well on transfection of an active siRNA (siDcstamp) that interferes Dcstamp mRNA expression, leading to impeded preosteoclast fusion and bone resorption, as well as promoted osteogenesis. In vivo results corroborate the abundant siDcstamp accumulation on bone surfaces and the enhanced trabecular bone mass volume and microstructure in treating osteoporotic OVX mice by rebalancing bone resorption, formation, and vascularization. Our study validates the hypothesis that satisfied transfection of siRNA enables preserved preosteoclasts that regulate bone resorption and formation simultaneously as potential anabolic treatment for osteoporosis.

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Section: Resultsmentioning

confidence: 99%

Rational polyelectrolyte nanoparticles endow preosteoclast-targeted siRNA transfection for anabolic therapy of osteoporosis

et al. 2023

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“…In addition, the efficient mass transfer ensures uniform distribution of reactants, minimizing the formation of undesirable by-products. 39,40…”

Section: Enhanced Mass and Heat Transfer: Catalyzing Efficient Polyme...mentioning

confidence: 99%

Controlled polymerization in microfluidics: Advancement in nanogel synthesis

Pandey,

Sharma,

Sandhu

et al. 2023

SPE Polymers

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This comprehensive review provides a detailed examination of controlled polymerization in the development of nanogels‐based microfluidic devices. Here, we have explored the integration of atom transfer radical polymerization (ATRP) and reversible addition‐fragmentation chain transfer (RAFT) techniques within microfluidic platforms for the synthesis of nanogels. The synergistic combination of ATRP and RAFT with microfluidics has emerged as a powerful tool for precise control over polymerization reactions, enabling the fabrication of well‐defined, multifunctional nanogels with tailored properties. The review begins with a thorough introduction to the principles of ATRP and RAFT, highlighting their respective advantages in controlled radical polymerization. Subsequently, it elucidates the principles of microfluidics and its profound impact on polymerization processes. The merging of these techniques enables precise control over reaction kinetics, monomer conversions, and molecular weight distributions, facilitating the synthesis of nanogels with unprecedented precision and reproducibility. Furthermore, this review delves into the chemical mechanisms underlying ATRP and RAFT reactions in microfluidic environments, emphasizing the role of various initiators, catalysts, and chain transfer agents. Special attention is given to the impact of microscale flow conditions on reaction kinetics and polymer structure. In addition, the review discusses emerging trends in the field, such as the incorporation of novel monomers and the exploration of environmentally benign reaction conditions.Highlights The controlled polymerization is the mechanism by which we can obtain tailormade material. The nanogel synthesis must ensure the gelation to be in confined dimensions here microfluidics plays key role.

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“…Polytetrahydrofuran (PTMG) is a biocompatible thermoplastic elastomer with low melting temperature ( T m , 25 °C), good resilience, high fungal resistance, and hydrolytic stability. , Moreover, poly(acrylic acid) (PAA) is an inexpensive green material for fabricating hydrogel and has attracted increasing attention due to its exceptional water absorption. , In this work, a versatile PAA–PTMG–PAA (diPAA–PTMG) polymer is synthesized as a switching phase and healing accelerator to construct a self-healing PVA blend boosted via shape memory. Watery and thermal stimulus shape memory and self-healing behaviors are investigated by regulating the di PAA–PTMG composition and PVA/ di PAA–PTMG proportion, and the working mechanism is explored.…”

Section: Introductionmentioning

confidence: 99%

Self-Healing of Poly(vinyl Alcohol)/Poly(acrylic Acid)–Polytetrahydrofuran–Poly(acrylic Acid) Blend Boosted via Shape Memory

Ren,

Song,

Chen

et al. 2023

Langmuir

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The self-healable polymers that can repair physical damage autonomously to extend their lifetime and reduce maintenance costs are promising intelligent materials. However, utilizing shape memory to facilitate self-repair is unusual at present. In this work, a series of poly(acrylic acid)–polytetrahydrofuran–poly(acrylic acid) polymers (PAA–PTMG–PAA, diPAA–PTMG) are synthesized as a switching phase and healing accelerator to blend into poly(vinyl alcohol) (PVA). The water swelling rate of the blend is up to 400.0% at 1/1 molecular weight ratio of PTMG/PAA and 20.0 wt % blend ratio of diPAA–PTMG to PVA, and its crystallization is changed significantly under wet conditions. The blend membrane exhibits not only a good hydrothermal-response shape memory effect but also a favorable self-healing behavior. The tensile strength and elongation at break are 12.4 MPa and 320.0% after healing at 25 °C, respectively. In particular, the wound membrane can achieve a better self-healing effect with the assistance of shape memory at 37 °C, and the elongation at the break increased to 515.9% after healing. The membrane is not cytotoxic, so it will be a promising biomedical material.

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Synthesis of Anionic Nanogels for Selective and Efficient Enzyme Encapsulation

Cited by 13 publications

References 53 publications

Rational polyelectrolyte nanoparticles endow preosteoclast-targeted siRNA transfection for anabolic therapy of osteoporosis

Rational polyelectrolyte nanoparticles endow preosteoclast-targeted siRNA transfection for anabolic therapy of osteoporosis

Controlled polymerization in microfluidics: Advancement in nanogel synthesis

Self-Healing of Poly(vinyl Alcohol)/Poly(acrylic Acid)–Polytetrahydrofuran–Poly(acrylic Acid) Blend Boosted via Shape Memory

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