Synthesis of antimicrobial silver nanoparticles through a photomediated reaction in an aqueous environment

Banasiuk, Rafał; Frackowiak, Janusz; Krychowiak, Marta; Matuszewska, Marta; Kawiak, Anna; Ziąbka, Magdalena; Lendzion-Bieluń, Zofia; Narajczyk, Magdalena; Królicka, Aleksandra

doi:10.2147/ijn.s93611

Cited by 21 publications

(4 citation statements)

References 24 publications

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“…Following ethanol dehydration, probes were embedded in Epon resin (Sigma-Aldrich), cut on a ultramicrotome Leica UC7 and contrasted in uranyl acetate and lead citrate. Transmission electron microscopy studies were performed using a Philips CM100 electron microscope ( Banasiuk et al, 2016 ).…”

Section: Methodsmentioning

confidence: 99%

Antifungal Activity and Mechanism of Action of the Co(III) Coordination Complexes With Diamine Chelate Ligands Against Reference and Clinical Strains of Candida spp.

et al. 2018

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Although many antifungal agents are available in clinical treatment, increasing resistance of fungi, especially Candida species, to the available drugs requires the development of new safe and non-toxic compounds with novel modes of action as effective treatment against resistant microorganisms. Cobalt complexes are very interesting and attractive as potential candidates with antimicrobial activity. Their therapeutic uses as antiviral, antibacterial antifungal, antiparasitic, antitumour, transferrin transporters, and anti-inflammatory agents are being intensively investigated. In this study we examined the antifungal activity of Co(III) complexes with diamine chelate ligands against a broad spectrum of Candida species. Minimum inhibitory concentration was determined by the microbroth dilution method and with serial passaging assay; the synergistic antimicrobial activity of the tested complexes combined with two antifungal drugs (ketoconazole and amphotericin B) was made by checkerboard assay. The effects of Co(III) complexes on yeast cell morphology were studied by optical and transmission electron microscopy. The mode of action of Co(III) complexes on the yeast cell wall (sorbitol assay) and cell membrane (ergosterol assay) were investigated. The cytotoxic effects of the tested compounds on red blood cells and the human keratinocyte (HaCaT) cell line were also evaluated. The analyzed compounds revealed significant antifungal activity for selected strains of Candida species; [CoCl2(dap)2]Cl (1) and [CoCl2(en)2]Cl (2) were more effective than ketoconazole. Its probable mechanism of action did not involve the cell wall or ergosterol binding. However, the checkerboard assay showed, that the antifungal activity of ketoconazole increased in combination with the tested complexes of Co(III). Our results suggest that both diamine complexes with Co(III) analogs caused damage to mitochondrial membrane or the membrane of the endoplasmic reticulum. The effect was observed by transmission electron microscope. Co(III) complexes with diamine chelate ligands are non-toxic at concentrations active against Candida species. This study provides new data on potential antifungal drugs, especially against Candida species.

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Section: Methodsmentioning

confidence: 99%

Antifungal Activity and Mechanism of Action of the Co(III) Coordination Complexes With Diamine Chelate Ligands Against Reference and Clinical Strains of Candida spp.

et al. 2018

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“…The reaction was performed in an aqueous environment and utilizes light emitted by commercially available 1 W light-emitting diodes (λ = 420 nm) as the catalyst. A detailed description of AgNPs-2 synthesis and their structural and chemical parameters is represented in [ 31 ].…”

Section: Methodsmentioning

confidence: 99%

Antimicrobial Activity of Two Different Types of Silver Nanoparticles against Wide Range of Pathogenic Bacteria

Holubnycha,

Husak,

Korniienko

et al. 2024

Nanomaterials

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The emergence of antibiotic-resistant bacteria, particularly the most hazardous pathogens, namely Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE)-pathogens pose a significant threat to global health. Current antimicrobial therapies, including those targeting biofilms, have shown limited effectiveness against these superbugs. Nanoparticles, specifically silver nanoparticles (AgNPs), have emerged as a promising alternative for combating bacterial infections. In this study, two types of AgNPs with different physic-chemical properties were evaluated for their antimicrobial and antibiofilm activities against clinical ESKAPE strains. Two types of silver nanoparticles were assessed: spherical silver nanoparticles (AgNPs-1) and cubic-shaped silver nanoparticles (AgNPs-2). AgNPs-2, characterized by a cubic shape and higher surface-area-to-volume ratio, exhibited superior antimicrobial activity compared to spherical AgNPs-1. Both types of AgNPs demonstrated the ability to inhibit biofilm formation and disrupt established biofilms, leading to membrane damage and reduced viability of the bacteria. These findings highlight the potential of AgNPs as effective antibacterial agents against ESKAPE pathogens, emphasizing the importance of nanoparticle characteristics in determining their antimicrobial properties. Further research is warranted to explore the underlying mechanisms and optimize nanoparticle-based therapies for the management of infections caused by antibiotic-resistant bacteria.

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“…The reaction took place in an aqueous solution, using light from commercially available 1 W light-emitting diodes (λ = 420 nm) as the catalyst. A detailed description of the synthesis of AgNPs-2, along with their structural and chemical parameters, can be found in [ 18 ]. The AgNPs utilized in the experiment exhibit a spherical morphology, with an average size of 27 ± 4.3 nm [ 13 ].…”

Section: Methodsmentioning

confidence: 99%

Antibacterial Potential and Biocompatibility of Chitosan/Polycaprolactone Nanofibrous Membranes Incorporated with Silver Nanoparticles

Korniienko,

Husak,

Diedkova

et al. 2024

Polymers

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This study addresses the need for enhanced antimicrobial properties of electrospun membranes, either through surface modifications or the incorporation of antimicrobial agents, which are crucial for improved clinical outcomes. In this context, chitosan—a biopolymer lauded for its biocompatibility and extracellular matrix-mimicking properties—emerges as an excellent candidate for tissue regeneration. However, fabricating chitosan nanofibers via electrospinning often challenges the preservation of their structural integrity. This research innovatively develops a chitosan/polycaprolactone (CH/PCL) composite nanofibrous membrane by employing a layer-by-layer electrospinning technique, enhanced with silver nanoparticles (AgNPs) synthesized through a wet chemical process. The antibacterial efficacy, adhesive properties, and cytotoxicity of electrospun chitosan membranes were evaluated, while also analyzing their hydrophilicity and nanofibrous structure using SEM. The resulting CH/PCL-AgNPs composite membranes retain a porous framework, achieve balanced hydrophilicity, display commendable biocompatibility, and exert broad-spectrum antibacterial activity against both Gram-negative and Gram-positive bacteria, with their efficacy correlating to the AgNP concentration. Furthermore, our data suggest that the antimicrobial efficiency of these membranes is influenced by the timed release of silver ions during the incubation period. Membranes incorporated starting with AgNPs at a concentration of 50 µg/mL effectively suppressed the growth of both microorganisms during the early stages up to 8 h of incubation. These insights underscore the potential of the developed electrospun composite membranes, with their superior antibacterial qualities, to serve as innovative solutions in the field of tissue engineering.

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Synthesis of antimicrobial silver nanoparticles through a photomediated reaction in an aqueous environment

Cited by 21 publications

References 24 publications

Antifungal Activity and Mechanism of Action of the Co(III) Coordination Complexes With Diamine Chelate Ligands Against Reference and Clinical Strains of Candida spp.

Antifungal Activity and Mechanism of Action of the Co(III) Coordination Complexes With Diamine Chelate Ligands Against Reference and Clinical Strains of Candida spp.

Antimicrobial Activity of Two Different Types of Silver Nanoparticles against Wide Range of Pathogenic Bacteria

Antibacterial Potential and Biocompatibility of Chitosan/Polycaprolactone Nanofibrous Membranes Incorporated with Silver Nanoparticles

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