Synthesis of bio-mediated silver nanoparticles from Silybum marianum and their biological and clinical activities

Shah, Muzamil; Nawaz, Sabir; Jan, Hasnain; Uddin, Noor; Ali, Ashaq; Anjum, Sumaira; Giglioli-Guivarc’h, Nathalie; Hano, Christophe; Abbasi, Bilal Haider

doi:10.1016/j.msec.2020.110889

Cited by 102 publications

(91 citation statements)

References 86 publications

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“…Orientin and chlorogenic acid protect the plant from stress conditions and perform various biological activities such as antioxidant, antiaging, anti-inflammatory, antidiabetic, antifungal, antibacterial, hepatoprotective, and anticancer (Lam et al, 2016;Naveed et al, 2018). The presence of flavonoids and hydroxycinnamic acid derivatives on the surface of nanoparticles is according to the results of our previous reports (Jan et al, 2020;Shah et al, 2020). These plant active compounds are considered to be involved in the capping of ZnO-NPs, as shown in Figure 2.…”

Section: Biocompatibility Of Zno Nanoparticles With Rbcsmentioning

confidence: 77%

Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora)

et al. 2020

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Herein, we report a facile, economic, one-pot green synthesis of zinc oxide nanoparticles (ZnO-NPs) for diverse biomedical applications. In the study, ZnO-NPs were synthesized using an aqueous extract of Aquilegia pubiflora as an effective reducing and capping agent. The biomediated nanoparticles were characterized using various techniques including high-performance liquid chromatography (HPLC), X-ray diffraction, Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy, Dynamic light scattering, photoluminescence, and RAMAN. The particles were highly pure, having an average size of 34.23 nm with spherical or elliptical morphology, and displayed good aqueous dispersion capability. FTIR and HPLC confirmed the successful capping of flavonoids and hydroxycinnamic acid derivatives. The characterized NPs were then explored for their antimicrobial and anti-leishmanial potential. Among the tested bacterial and fungal strains, ZnO-NPs were more potent toward Pseudomonas aeruginosa and Fusarium solani with inhibition zone of 10.3 ± 0.19 mm and 13 ± 1.4 mm, respectively. A dose-dependent cytotoxic effect was observed against Leishmania tropica (KWH23) with significant IC 50 for both the promastigote (48 µg/mL) and amastigote form (51 µg/mL) of the parasite. In addition, bacterial kinase enzymes were inhibited by ZnO-NPs, thus allowing us to elaborate a possible action mechanism. Finally, the remarkable biocompatible nature of the particles was confirmed against freshly isolated human red blood cells (hRBCs). Altogether, these results affirmed the high antimicrobial and antiparasitic potential of ZnO-NPs obtained through a biogenic synthetic approach using aqueous extract of the Himalayan Columbine (Aquilegia pubiflora).

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Section: Biocompatibility Of Zno Nanoparticles With Rbcsmentioning

confidence: 77%

Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora)

et al. 2020

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“…In these extracts, phytoconstituents such as polyphenols are believed to be responsible for the reduction and stabilization of the AgNPs. To identify them, Fourier-transform infrared (FTIR) spectroscopy was employed [ 19 ] in addition to nuclear magnetic resonance (NMR) spectroscopy [ 20 ] and high-performance liquid chromatography coupled with mass detector (LC-MS) [ 21 ]. Fractions or pure natural compounds have also been used in the green synthesis of nanoparticles [ 22 ] and examples include tannic acid [ 23 ], quercetin, gallic acid [ 24 ], and other compounds [ 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

Biosynthesis, Characterization, and Biological Activities of Procyanidin Capped Silver Nanoparticles

Badeggi

Badmus

Botha

et al. 2020

JFB

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In this study, procyanidin dimers and Leucosidea sericea total extract (LSTE) were employed in the synthesis of silver nanoparticles (AgNPs) and characterized by ultraviolet-visible (UV-Visible) spectroscopy, high-resolution transmission electron microscopy (HRTEM), selected area electron diffraction (SAED), X-ray diffraction (XRD), and dynamic light scattering (DLS) techniques. AgNPs of about 2–7 nm were obtained. DLS and stability evaluations confirmed that the AgNPs/procyanidins conjugates were stable. The formed nanoparticles exhibited good inhibitory activities against the two enzymes studied. The IC50 values against the amylase enzyme were 14.92 ± 1.0, 13.24 ± 0.2, and 19.13 ± 0.8 µg/mL for AgNPs coordinated with LSTE, F1, and F2, respectively. The corresponding values for the glucosidase enzyme were 21.48 ± 0.9, 18.76 ± 1.0, and 8.75 ± 0.7 µg/mL. The antioxidant activities were comparable to those of the intact fractions. The AgNPs also demonstrated bacterial inhibitory activities against six bacterial species. While the minimum inhibitory concentrations (MIC) of F1-AgNPs against Pseudomonas aeruginosa and Staphylococcus aureus were 31.25 and 15.63 µg/mL respectively, those of LSTE-AgNPs and F2-AgNPs against these organisms were both 62.50 µg/mL. The F1-AgNPs demonstrated a better bactericidal effect and may be useful in food packaging. This research also showed the involvement of the procyanidins as reducing and capping agents in the formation of stable AgNPs with potential biological applications.

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“…Free radical scavenging assay (FRSA) using DPPH (2,2-diphenyl-1-picrylhydrazyl) was determined following the reported protocol [75] with slight modifications. Sample extract (20 µL) was mixed with DPPH (3.2 mg/100 mL methanol) 180 µL and the mixture was then incubated for 60 min at 25 ± 2 °C followed by dH2O (160 µL) addition.…”

Section: Dpph Assaymentioning

confidence: 99%

Chitosan Elicitation Impacts Flavonolignan Biosynthesis in Silybum marianum (L.) Gaertn Cell Suspension and Enhances Antioxidant and Anti-inflammatory Activities of Cell Extracts

Shah¹,

Jan²,

Drouet³

et al. 2021

Preprint

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Silybum marianum (L.) Gaertn is a rich source of antioxidants and anti-inflammatory flavonolignans with great potential for use in pharmaceutical and cosmetic products. Its biotechnological production using in vitro culture system has been proposed. Chitosan is a well-known elicitor that strongly affects both secondary metabolites and biomass production by plants. The effect of chitosan on S. marianum cell suspension is not known yet. In the present study, suspension cultures of S marianum were exploited for their in vitro potency to produce bioactive flavonolignans in the presence of chitosan. Established cell suspension culture was maintained on the same hormonal media supplemented with 0.5 mg/L BAP (6-benzylaminopurine) and 1.0 mg/L NAA (α-naphthalene acetic acid) under photoperiod 16/8 h (light/dark) and exposed to various treatments of chitosan (ranging from 0.5 to 50.0 mg/L). The highest biomass production was observed for cell suspension treated with 5.0 mg/L chitosan, resulting in 123.3 g/L fresh weight (FW) and 17.7 g/L dry weight (DW) productions. Chitosan treatment resulted in an overall increase in the accumulation of flavonoids, phenolic compounds and silymarin. High accumulation levels of silybin B, silydianin and silybin A were recorded by HPLC analysis. The corresponding extracts displayed interesting antioxidant and anti-inflammatory capacities. In particular, high ABTS antioxidant activity (741.5 μM Trolox C equivalent antioxidant capacity) was recorded in extracts obtained in presence of 0.5 mg/L of chitosan. On the opposite, highest inhibitions of cyclooxygenase 2 (COX-2, 30.5 %), secretory phospholipase A2 (sPLA2, 33.9 %) and 15-lipoxygenase (15-LOX-2, 31.6 %) enzymes involved in inflammation process were measured in extracts obtained in presence of 5.0 mg/L of chitosan. Taken together, these results highlight the high potential of the chitosan elicitation of the S. marianum cell suspension for enhanced production of antioxidant and anti-inflammatory silymarin-rich extracts.

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Synthesis of bio-mediated silver nanoparticles from Silybum marianum and their biological and clinical activities

Cited by 102 publications

References 86 publications

Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora)

Biogenic Synthesis and Characterization of Antimicrobial and Antiparasitic Zinc Oxide (ZnO) Nanoparticles Using Aqueous Extracts of the Himalayan Columbine (Aquilegia pubiflora)

Biosynthesis, Characterization, and Biological Activities of Procyanidin Capped Silver Nanoparticles

Chitosan Elicitation Impacts Flavonolignan Biosynthesis in Silybum marianum (L.) Gaertn Cell Suspension and Enhances Antioxidant and Anti-inflammatory Activities of Cell Extracts

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