Synthesis of Cell‐Laden Alginate Hollow Fibers Using Microfluidic Chips and Microvascularized Tissue‐Engineering Applications

Lee, Kwang Ho; Shin, Suji; Park, Yongdoo; Lee, Sang Hoon

doi:10.1002/smll.200801667

Cited by 204 publications

(158 citation statements)

References 14 publications

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“…We reported the first example of a microfluidic spinning process using a microfluidic chip that integrated a tapered glass capillary (Jeong et al, 2004). Diverse hydrogel-based solids and hollow fibers were fabricated using variations on these chip designs (Shin et al, 2007;Hwang et al, 2008Hwang et al, , 2009Lee et al, 2009Lee et al, , 2010). Stupp's group used a tapered pipet to prepare noodle-like strings in which peptide amphiphile nanofibers aligned and were bundled along the longitudinal direction of the string .…”

Section: Spinning Via a Tapered Duct-based Elongational Mechanismmentioning

confidence: 99%

Development of New Smart Materials and Spinning Systems Inspired by Natural Silks and Their Applications

Cheng

Lee

2016

Front. Mater.

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Silks produced by spiders and silkworms are charming natural biological materials with highly optimized hierarchical structures and outstanding physicomechanical properties. The superior performance of silks relies on the integration of a unique protein sequence, a distinctive spinning process, and complex hierarchical structures. Silks have been prepared to form a variety of morphologies and are widely used in diverse applications, for example, in the textile industry, as drug delivery vehicles, and as tissue engineering scaffolds. This review presents an overview of the organization of natural silks, in which chemical and physical functions are optimized, as well as a range of new materials inspired by the desire to mimic natural silk structure and synthesis.

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Section: Spinning Via a Tapered Duct-based Elongational Mechanismmentioning

confidence: 99%

Development of New Smart Materials and Spinning Systems Inspired by Natural Silks and Their Applications

Cheng

Lee

2016

Front. Mater.

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“…The acetic acid in the oil phase diffuses into the droplets and triggers the release of calcium ions, which leads to the gelation of the droplets to form alginate particles. Note that the incorporation of CaCO 3 nanoparticles allows the internal gelation of alginate droplets and avoids the generation of undesired shapes of particles; (C) Experimental setup for the encapsulation of cells into poly(ethylene glycol) diacrylate particles with desired shapes using the stop-flow lithography [26]; (D) Microfluidic setup to generate alginate hollow microfibers [102]. In the experiment, a three-layer of coaxial flow is generated along the channel.…”

Section: Figure 2 (A) (Above)mentioning

confidence: 99%

“…In addition, cell-containing microfibers can be woven to obtain three-dimensional scaffolds, such as cell sheets [101], or used to generate tubular structures (hollow fibers) for vascular tissue engineering [102]. Therefore, fibrous materials are highly desired for tissue engineering and cell-based therapy.…”

Section: Microfluidic-based Synthesis Of Hydrogel Fibers For Cell Micmentioning

confidence: 99%

“…The cells aggregate as spheroid-like structures in the fiber and their hepatic functions in terms of albumin secretion and urea synthesis are found to be higher than the cells that are cultured on uncoated polystyrene dishes. Meanwhile, because multiphase streams in glass capillary microfluidic channels are co-axial and can form core-shell jests, hollow microfibers can be fabricated if the core fluid is non-polymerizable but the shell fluid is polymerizable [102,110]. Indeed, alginate hollow fibers are fabricated by using a glass capillary microfluidic setup where the core, shell, and sheath fluid are CaCl 2 , alginate, and CaCl 2 solution, respectively ( Figure 2D) [102].…”

Section: Microfluidic-based Synthesis Of Hydrogel Fibers For Cell Micmentioning

confidence: 99%

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Microfluidic-Based Synthesis of Hydrogel Particles for Cell Microencapsulation and Cell-Based Drug Delivery

2012

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Encapsulation of cells in hydrogel particles has been demonstrated as an effective approach to deliver therapeutic agents. The properties of hydrogel particles, such as the chemical composition, size, porosity, and number of cells per particle, affect cellular functions and consequently play important roles for the cell-based drug delivery. Microfluidics has shown unparalleled advantages for the synthesis of polymer particles and been utilized to produce hydrogel particles with a well-defined size, shape and morphology. Most importantly, during the encapsulation process, microfluidics can control the number of cells per particle and the overall encapsulation efficiency. Therefore, microfluidics is becoming the powerful approach for cell microencapsulation and construction of cell-based drug delivery systems. In this article, I summarize and discuss microfluidic approaches that have been developed recently for the synthesis of hydrogel particles and encapsulation of cells. I will start by classifying different types of hydrogel material, including natural biopolymers and synthetic polymers that are used for cell encapsulation, and then focus on the current status and challenges of microfluidic-based approaches. Finally, applications of cell-containing hydrogel particles for cell-based drug delivery, particularly for cancer therapy, are discussed.

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“…11 In particular, the selectivity of sequences of guluronic acid residues to interact with Ca 2+ and induce gelation 12 under mild, physiologically compatible conditions, are widely explored. Use of alginate has for instance been demonstrated for encapsulation of cells for tissue engineering purposes [13][14][15][16] and has been investigated for treatment of diabetes mellitus type I. 17,18 However, a number of technical barriers currently remain which limit the applicability of the Ca 2+ induced gelation in microfluidics, including.…”

Section: Introductionmentioning

confidence: 99%

Versatile, cell and chip friendly method to gel alginate in microfluidic devices

et al. 2016

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Alginate is used extensively in microfluidic devices to produce discreet beads or fibres at the microscale. Such structures may be used to encapsulate sensitive cargoes such as cells and biomolecules. On chip gelation of alginate represents a significant challenge since gelling kinetics or physicochemical conditions are not biocompatible. Here we present a new method that offers a hitherto unprecedented level of control over the gelling kinetics and pH applied to the encapsulation of a variety of cells in both bead and fibre geometries. This versatile approach proved extremely simple to implement and resulted in highly reliable device operation and very high viability of several different encapsulated cell types. We believe this method offers a paradigm shift in alginate gelling technology for application in microfluidics.

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Synthesis of Cell‐Laden Alginate Hollow Fibers Using Microfluidic Chips and Microvascularized Tissue‐Engineering Applications

Cited by 204 publications

References 14 publications

Development of New Smart Materials and Spinning Systems Inspired by Natural Silks and Their Applications

Development of New Smart Materials and Spinning Systems Inspired by Natural Silks and Their Applications

Microfluidic-Based Synthesis of Hydrogel Particles for Cell Microencapsulation and Cell-Based Drug Delivery

Versatile, cell and chip friendly method to gel alginate in microfluidic devices

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