Synthesis of collagen by human fibroblasts and their SV40 transformants

Krieg, Thomas; Aumailley, Monique; Dessau, Waltraud; Wiestner, Manfred; Müller, Peter

doi:10.1016/0014-4827(80)90184-6

Cited by 56 publications

(19 citation statements)

References 24 publications

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“…Krieg et al observed a 3-8-fold decrease in collagen synthesis and a slight increase in the intracellular collagen degradation in SV-40-transformed human fibroblasts as compared to their normal controls [10]. We have found in our previous experiments carried out on the same cell strains as in the present study, a 3-fold reduction in the rates of synthesis of collagenase digestible [14C]peptides per cell in SV-40-transformed fibroblasts [3].…”

Section: Data Insupporting

confidence: 68%

Secretion and intracellular degradation of collagen in cultures of normal and SV‐40‐transformed human fibroblasts

Berman

Morozevich

1990

FEBS Letters

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With a pulse-chase technique, secretion and intracellular degradation of collagen were investigated in human cultured normal and SV-40-transformed fibroblasts. Normal cells at a proliferative phase of growth secreted collagen more actively than the stationary phase (resting) cells. Transformed fibroblasts secreted protein at a lower rate than both normal cell types. Resting and dividing normal cells displayed no differences in the rates of intracellular collagen degradation at the various stages of the chase period. Transformed cells did not differ from the normal ones in collagen degradation rates at the first hour of the chase period while at later times in the chase period, the total amount of degraded collagen was reduced by 2(~30% in the transformed vs normal cultures. The data are discussed in the view of possible relations between the various mechanisms of intracellular transport and degradation of collagen.

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Section: Data Insupporting

confidence: 68%

Secretion and intracellular degradation of collagen in cultures of normal and SV‐40‐transformed human fibroblasts

Berman

Morozevich

1990

FEBS Letters

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“…Adult human skin fibroblasts, established by outgrowth of biopsies from healthy donors or scleroderma patients as previously described (20), were used in passage 3-6. Cells were maintained in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal calf serum (FCS), 50 g/ml sodium ascorbate, 300 g/ml glutamine, 100 U/ml penicillin, 100 g/ml streptomycin, and grown in the atmosphere of a CO 2 incubator at 37°C.…”

Section: Methodsmentioning

confidence: 99%

Effect of Interleukin-10 on the Gene Expression of Type I Collagen, Fibronectin, and Decorin in Human Skin Fibroblasts: Differential Regulation by Transforming Growth Factor-β and Monocyte Chemoattractant Protein-1

Yamamoto

Eckes

Krieg

2001

Biochemical and Biophysical Research Communications

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130

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“…The cells were grown in Dulbecco's modified Eagle's medium (DMEM) supplemented with 1007o fetal calf serum, sodium ascorbate (50/~g/ml), glutamine (300/~g/ml), penicillin (400 U/ml) and streptomycin (50/~g/ml) in plastic Petri dishes in the moist atmosphere of a CO2 incubator (5070 CO,, 95070 air) at 37°C [18]. Confluent monolayers were propagated by trypsinization (0.1 070 trypsin, 0.02070 EDTA) and replating at 1:2 dilution.…”

Section: Methodsmentioning

confidence: 99%

Collagenase gene expression in fibroblasts is regulated by a three‐dimensional contact with collagen

Mauch¹,

Adelmann-Grill

Hatamochi³

et al. 1989

FEBS Letters

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111

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Collagenase activity in fibroblasts is regulated by cytokines and the interaction with the extracellular matrix. In this study we demonstrate that fibroblasts cultured within a three-dimensional collagen gel show a strong induction of collagenase gene expression. In addition to increased de novo synthesis most of the secreted enzyme was found to be activated leading to a high collagenolytic activity and complete degradation of collagen matrices after removal of fetal calf serum. Collagen I gene expression was found to be reduced under these conditions. These data suggest a specific modulation of cellular metabolism in response to contact with a three-dimensional collagenous matrix resulting in the divergent regulation of collagen and collagenase.

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Synthesis of collagen by human fibroblasts and their SV40 transformants

Cited by 56 publications

References 24 publications

Secretion and intracellular degradation of collagen in cultures of normal and SV‐40‐transformed human fibroblasts

Secretion and intracellular degradation of collagen in cultures of normal and SV‐40‐transformed human fibroblasts

Effect of Interleukin-10 on the Gene Expression of Type I Collagen, Fibronectin, and Decorin in Human Skin Fibroblasts: Differential Regulation by Transforming Growth Factor-β and Monocyte Chemoattractant Protein-1

Collagenase gene expression in fibroblasts is regulated by a three‐dimensional contact with collagen

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