Synthesis of composite gelatin-hyaluronic acid-alginate porous scaffold and evaluation for in vitro stem cell growth and in vivo tissue integration

Singh, Deepti; Tripathi, Anuj; Zo, Sunmi; Singh, Dinesh; Han, Sung Soo

doi:10.1016/j.colsurfb.2014.01.049

Cited by 61 publications

(29 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Gelatin is a hydrolyzed product from collagen with desired features of prompting hemostasis and guiding cell adherence, proliferation and differentiation. 15,20 Combining two or more different polymers by cross-linkers has been reported to be an effective method to create a new scaffold, which can get rid of their disadvantages and integrate their advantages. 21,22 For example, the composite materials blending alginate with gelatin together have gained much attention because of superior physicochemical and biological properties.…”

Section: Introductionmentioning

confidence: 99%

Hybrid cellulose nanocrystal/alginate/gelatin scaffold with improved mechanical properties and guided wound healing

Shan

et al. 2019

RSC Adv.

View full text Add to dashboard Cite

show abstract

Section: Introductionmentioning

confidence: 99%

Hybrid cellulose nanocrystal/alginate/gelatin scaffold with improved mechanical properties and guided wound healing

Shan

et al. 2019

RSC Adv.

View full text Add to dashboard Cite

show abstract

“…In another work, Singh et al designed a 3D macroporous material based on HA, gelatin, and alginate, which was crosslinked using calcium chloride. 254 The component selection was based on the unique properties that each biopolymers provided: gelatin was chosen to promote cell adhesion and cell-cell interactions, alginate to provide good encapsulation properties and inertness towards cells, and HA to enhance stem cell migration and differentiation and promote osteogenesis. The composite hydrogel promoted the osteogenic differentiation of stem cells for bone tissue engineering in vivo.…”

Section: Gelatin-hyaluronic Acidmentioning

confidence: 99%

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

Afewerki

Sheikhi

Kannan

et al. 2018

Bioengineering & Transla Med

309

210

View full text Add to dashboard Cite

Gelatin is a promising material as scaffold with therapeutic and regenerative characteristics due to its chemical similarities to the extracellular matrix (ECM) in the native tissues, biocompatibility, biodegradability, low antigenicity, cost‐effectiveness, abundance, and accessible functional groups that allow facile chemical modifications with other biomaterials or biomolecules. Despite the advantages of gelatin, poor mechanical properties, sensitivity to enzymatic degradation, high viscosity, and reduced solubility in concentrated aqueous media have limited its applications and encouraged the development of gelatin‐based composite hydrogels. The drawbacks of gelatin may be surmounted by synergistically combining it with a wide range of polysaccharides. The addition of polysaccharides to gelatin is advantageous in mimicking the ECM, which largely contains proteoglycans or glycoproteins. Moreover, gelatin–polysaccharide biomaterials benefit from mechanical resilience, high stability, low thermal expansion, improved hydrophilicity, biocompatibility, antimicrobial and anti‐inflammatory properties, and wound healing potential. Here, we discuss how combining gelatin and polysaccharides provides a promising approach for developing superior therapeutic biomaterials. We review gelatin–polysaccharides scaffolds and their applications in cell culture and tissue engineering, providing an outlook for the future of this family of biomaterials as advanced natural therapeutics.

show abstract

“…HA plays an essential role in the composition and structure of the extracellular matrix (ECM), which is mainly composed of GAGs and collagens through covalent and non-covalent interactions [5][6][7], could regulate cells adhesion, migration and morphogenesis, adjust cells proliferation and differentiation [8,9] and has important effects on the development, organization or remodeling of tissue, angiogenesis, modulation of inflammation and wound healing [10][11][12]. Because of its unique features, HA has been widely investigated and applied as biomaterials for drug and protein delivery [13][14][15], threedimensional (3D) scaffold for cells culture [16][17][18], cartilage and bone regeneration [12,19,20], wound healing [21,22], bio-printing [23,24], and so on. However, as a natural polymer, rapid degradation in vivo and poor biomechanical properties limited its biomedical applications to some extent [2,12].…”

Section: Introductionmentioning

confidence: 99%

The self-crosslinking smart hyaluronic acid hydrogels as injectable three-dimensional scaffolds for cells culture

Bian

Sui

et al. 2016

Colloids and Surfaces B: Biointerfaces

129

View full text Add to dashboard Cite

Although the disulfide bond crosslinked hyaluronic acid hydrogels have been reported by many research groups, the major researches were focused on effectively forming hydrogels. However, few researchers paid attention to the potential significance of controlling the hydrogel formation and degradation, improving biocompatibility, reducing the toxicity of exogenous and providing convenience to the clinical operations later on. In this research, the novel controllable self-crosslinking smart hydrogels with in-situ gelation property was prepared by a single component, the thiolated hyaluronic acid derivative (HA-SH), and applied as a three-dimensional scaffold to mimic native extracellular matrix (ECM) for the culture of fibroblasts cells (L929) and chondrocytes. A series of HA-SH hydrogels were prepared depending on different degrees of thiol substitution (ranging from 10 to 60%) and molecule weights of HA (0.1, 0.3 and 1.0 MDa). The gelation time, swelling property and smart degradation behavior of HA-SH hydrogel were evaluated. The results showed that the gelation and degradation time of hydrogels could be controlled by adjusting the component of HA-SH polymers. The storage modulus of HA-SH hydrogels obtained by dynamic modulus analysis (DMA) could be up to 44.6 kPa. In addition, HA-SH hydrogels were investigated as a three-dimensional scaffold for the culture of fibroblasts cells (L929) and chondrocytes cells in vitro and as an injectable hydrogel for delivering chondrocytes cells in vivo. These results illustrated that HA-SH hydrogels with controllable gelation process, intelligent degradation behavior, excellent biocompatibility and convenient operational characteristics supplied potential clinical application capacity for tissue engineering and regenerative medicine.

show abstract

Synthesis of composite gelatin-hyaluronic acid-alginate porous scaffold and evaluation for in vitro stem cell growth and in vivo tissue integration

Cited by 61 publications

References 30 publications

Hybrid cellulose nanocrystal/alginate/gelatin scaffold with improved mechanical properties and guided wound healing

Hybrid cellulose nanocrystal/alginate/gelatin scaffold with improved mechanical properties and guided wound healing

Gelatin‐polysaccharide composite scaffolds for 3D cell culture and tissue engineering: Towards natural therapeutics

The self-crosslinking smart hyaluronic acid hydrogels as injectable three-dimensional scaffolds for cells culture

Contact Info

Product

Resources

About