The regular utilization of medical bandages has the potential to expedite the healing process. In this research study, we explored the creation of an innovative medical textile in the form of a padded cotton bandage, engineered from a combination of agricultural biopolymers, with a focus on sustainability and eco-friendliness. To produce a cotton-based bandage, cotton fabric was treated with Arabic gum and xanthan gum loaded with Ag NPs. The resulting samples underwent assessment for air permeability, antibacterial attributes, tensile strength, and wicking properties across various biopolymer blend compositions. Furthermore, their characteristics were analyzed using Tensile strength Tester, SEM, and FTIR. Our study identified that a blend of 0.5% xanthan gum and 1% gum Arabic demonstrated the highest air permeability. Additionally, the analysis of tensile strength revealed that the sample with the highest GSM exhibited superior strength. SEM and FTIR analyses have verified the presence of the Ag-loaded biopolymer layer on cotton fabric. The 50 GSM sample exhibits superior wicking properties compared to the 100 GSM samples. This research presents a promising avenue for developing biopolymer-based padded cotton bandages with enhanced air permeability, antibacterial efficacy, tensile strength, and moisture-wicking properties, crucial for effective and comfortable medical textiles.