Synthesis of copper nanoclusters from Bacopa monnieri leaves for fluorescence sensing of dichlorvos

Abstract

In this work, a facile one‐step green synthesis is developed for the fabrication of blue fluorescent copper nanocluster (Brahmi‐CuNCs) from the extract of Bacopa monnieri (common name is Brahmi) via microwave method. The as‐prepared Brahmi‐CuNCs emit blue fluorescence at 452 nm when excited at 352 nm and show a quantum yield of 31.32%. Brahmi‐derived blue fluorescent CuNCs acted as a probe for fluorescence sensing of dichlorvos. Upon the addition of dichlorvos, blue emission of Brahmi‐CuNCs is gradually turned‐off, favoring to establish calibration graph in the range of 0.5‐100 μM with a detection limit of 0.23 μM, respectively. The as‐synthesized Brahmi‐CuNCs exhibited remarkable sensitivity and selectivity toward dichlorvos, offering to assay dichlorvos in various samples (cabbage, apple juice, and rice).

Copper Ion-Induced Self-Assembled Aerogels of Carbon Dots as Peroxidase-Mimicking Nanozymes for Colorimetric Biosensing of Organophosphorus Pesticide

“…On the basis of the LOD value, the linear range, specificity, reproducibility, cost, and detection time, the performance of the proposed sensor for DDVP detection is superior to or well comparable with those previously reported sensors (Table S2). − ,, …”

“…On the basis of the LOD value, the linear range, specificity, reproducibility, cost, and detection time, the performance of the proposed sensor for DDVP detection is superior to or well comparable with those previously reported sensors (Table S2). [7][8][9][10][11]52,53 Real Sample Analysis. In order to validate the potential feasibility of the constructed biosensor for detecting DDVP in real samples, the concentrations of DDVP in real samples including cabbage, pakchoi, and lake water were assessed by the standard addition method and the reference gas chromatography (GC) method.…”

“…Sahub et al constructed a fluorescent sensor for dichlorvos detection in water samples using graphene quantum dots and active enzyme . Sadhu et al synthesized a fluorescent copper nanocluster from the extract of Bacopa monnieri by a microwave method and used it to construct a fluorescent sensor for dichlorvos detection in foods . However, the disadvantages of the methods, such as high cost and being time-consuming and having complex operational procedures, limit their applications.…”

“…10 microwave method and used it to construct a fluorescent sensor for dichlorvos detection in foods. 11 However, the disadvantages of the methods, such as high cost and being time-consuming and having complex operational procedures, limit their applications. The colorimetric method has a wide application in the detection of pesticides due to the advantages of low costs, a simple operation, and rapidity.…”

“…Environmental safety has become an important issue for public health and ecosystem vitality due to the harmful and detrimental consequences brought about by various pollutants emission to the environment. , Among various pollutants, organophosphorus pesticides (OPs) are a class of widely used lethal nerve agents, which can protect crops from pests and diseases in agricultural production. , However, the overuse of organophosphorus pesticides leads to high residues existing widely in food, water, and the environment. , These residual organophosphorus pesticides can irreversibly combine with the enzymes' active site of acetylcholinesterase (AChE) in living organisms to cause the buildup of neurotransmitter acetylcholine (ACh), which further poses a serious threat to human health. , Therefore, a simple, rapid, sensitive, and selective method for OP detection has become increasingly necessary. At present, there are many methods for OP detection, such as high-performance liquid chromatography, , electrochemistry, , and fluorescence. − Xu et al reported the use of high-performance liquid chromatography with an ultraviolet detector for the determination of trace dichlorvos residues in foods . Qin et al developed and validated a liquid chromatography-tandem mass spectrometry to determine dichlorvos in tobacco .…”