Synthesis of double-layered rotavirus-like particles using internal ribosome entry site vector system in stably-transformed Drosophila melanogaster

Lee, Jong Min; Chung, Ha Young; Kim, Kyung Il; Yoo, Ki Hyun; Jeon, Hoon; Chung, In Sik; Park, Jong-Hwa

doi:10.1007/s10529-010-0390-x

Cited by 16 publications

(10 citation statements)

References 15 publications

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“…Furthermore, Rodríguez‐Limas et al utilized yeast as an expression system for the first time to produce multilayered RV‐like particles with complex architecture by employing various molecular and process strategies . The wheel‐like VP2/6 double‐layered virus‐like particle has also been produced by employing an Encephalomyocarditis virus (EMCV)‐derived internal ribosomal entry site element, whereby VP2 and VP6 proteins were co‐expressed in stably transformed Drosophila melanogaster Schneider 2 (S2) cells . In another study, herpes simplex virus type 1 amplicon vectors were employed for the production of RV‐VLPs in mammalian cells .…”

Section: Rotavirus Virus‐like Particlesmentioning

confidence: 99%

“…105 The wheel-like VP2/6 double-layered virus-like particle has also been produced by employing an Encephalomyocarditis virus (EMCV)-derived internal ribosomal entry site element, whereby VP2 and VP6 proteins were co-expressed in stably transformed Drosophila melanogaster Schneider 2 (S2) cells. 109 In another study, herpes simplex virus type 1 amplicon vectors were employed for the production of RV-VLPs in mammalian cells. 110 These amplicon vectors co-expressed FIGURE 2 Schematic representation of production of non-enveloped and enveloped VLPs.…”

Section: Virus-like Particle (Vlp) -The Technologymentioning

confidence: 99%

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Rotavirus virus‐like particles (RV‐VLPs) vaccines: An update

Changotra

Vij

2017

Reviews in Medical Virology

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Rotaviruses (RVs) cause over 0.2 million deaths annually and are reported to be the foremost cause of gastroenteritis in infants and children worldwide. Vaccination against RVs is the most successful and unsurpassed strategy to combat infection to date. Although the 2 current vaccines, Rotarix and RotaTeq, have dramatically reduced the disease burden, still there is a need for new vaccines. In this context, RV virus-like particles (RV-VLPs) represent potential vaccine candidates as they are noninfectious and effective nonreplicating immunogens that may reduce the risk of side effects related to the conventional vaccines. VLPs being conformationally similar to the parent virus are highly immunogenic and hence provide enhanced protection and better serotype coverage. In this review, we have highlighted the various advantages and the implications of RV-VLPs, discussed the general strategies employed for their production, and talked about the recent developments made in this regard. Overall, the review emphasizes the probable utility of RV-VLPs in eradicating the highly widespread RVs.

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Section: Rotavirus Virus‐like Particlesmentioning

confidence: 99%

Section: Virus-like Particle (Vlp) -The Technologymentioning

confidence: 99%

Rotavirus virus‐like particles (RV‐VLPs) vaccines: An update

Changotra

Vij

2017

Reviews in Medical Virology

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“…Triplelayered rotavirus VLPs composed of capsid proteins, VP2, VP6 and VP7,have been produced in Sf-9 and Sf-21 cells using a recombinant baculovirus containing atricistronicgene expression cassette [51]. Double-layered rotavirus VLPs are also produced in stably transformed Drosophila melanogaster S2 cells using encephalomyocarditis-virusderived internal ribosomal entry site element [52]. Improved production of enterovirus 71 VLPs was performed by co-expression of P1 and 3CD protease under the control of polyhedrin and cytomegalovirus immediate early promoters, respectively, in a single recombinant bacmid [53].…”

Section: Insectsmentioning

confidence: 99%

Functional Virus-Like Particles Production Using Silkworm and Their Application in Life Science

Kato¹,

Deo²,

Park

et al. 2012

J Biotechnol Biomaterial

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Virus-like particles (VLPs), which mimic the structure of authentic virus, are of significant importance owing to their wide ranging applications. VLPs have the potential to serve as candidate vaccine in addition to subunit vaccines and also serve as a nano-bioparticle scaffold for displaying complex foreign proteins. Here, we review different methods available to produce VLPs with various host expression systems including from microorganisms to mammalian cells and the current potential of silkworms as producers of these VLPs. Recently, silkworms have been used for efficient production of VLPs too in addition to mass production of recombinant proteins, which have contributed to molecular structural analysis, molecule-molecule interactions in biology and biotechnology.

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“…Attention has been recently directed towards the production of VLPs by recombinant insect cells. Successful expressions have been demonstrated: Japanese encephalitis (JE) VLPs in a stably transformed Drosophila cell line (Zhang et al 2007); JE VLPs in Trichoplusia ni BTI-TN-5B1-4 (High Five) cells (Yamaji et al 2009(Yamaji et al , 2010; HIV-1 Pr55gag-based VLPs in High Five cells (Tagliamonte et al 2010); Rous sarcoma virus protein-based VLPs in High Five cells (Deo et al 2011); and, double-layered rotavirus-like particles in Drosophila melanogaster S2 cells (Lee et al 2011). When lepidopteran insect cells, such as Sf9 and High Five cells, are used as host cells for stable expression, the choice of a promoter to drive the heterologous gene expression is crucial as the use of weak promoters results in low recombinant protein yields (Yamaji 2011).…”

Section: Introductionmentioning

confidence: 99%

Efficient production of Japanese encephalitis virus-like particles by recombinant lepidopteran insect cells

Yamaji

Nakamura

Kuwahara

et al. 2012

Appl Microbiol Biotechnol

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The production of Japanese encephalitis (JE) virus-like particles (VLPs) in stably transformed lepidopteran insect cells was investigated. The DNA fragment encoding the JE virus (JEV) prM signal peptide, the precursor (prM) of the viral membrane protein (M), and the envelope glycoprotein (E) was cloned into the plasmid vector pIHAbla. The pIHAbla contained the Bombyx mori actin promoter downstream of the B. mori nucleopolyhedrovirus (BmNPV) IE-1 transactivator and the BmNPV HR3 enhancer for high-level expression, together with a blasticidin resistance gene for use as a selectable marker. DNA encoding a form of prM with a pr/M cleavage site mutation was used to suppress the cell-fusion activity of VLPs. After transfection with the resultant plasmid, Trichoplusia ni BTI-TN-5B1-4 (High Five) cells were incubated with blasticidin, and cells resistant to the antibiotic were obtained. Western blot analysis and enzyme-linked immunosorbent assay of a culture supernatant showed that transfected High Five cells secreted an E antigen equivalent to the authentic JEV E. Sucrose density-gradient sedimentation analysis of the culture supernatant from recombinant High Five cells indicated that secreted E antigen molecules were produced in a particulate form. VLPs recovered from the supernatant successfully induced neutralizing antibodies in mice, particularly when adsorbed to alum adjuvant. High yields (≈30 μg/ml) of E antigen were achieved in shake-flask cultures. These results indicate that recombinant insect cells may offer a novel approach for efficient VLP production.

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Synthesis of double-layered rotavirus-like particles using internal ribosome entry site vector system in stably-transformed Drosophila melanogaster

Cited by 16 publications

References 15 publications

Rotavirus virus‐like particles (RV‐VLPs) vaccines: An update

Rotavirus virus‐like particles (RV‐VLPs) vaccines: An update

Functional Virus-Like Particles Production Using Silkworm and Their Application in Life Science

Efficient production of Japanese encephalitis virus-like particles by recombinant lepidopteran insect cells

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