Synthesis of E.coli tRNA fMet fragments and their joining with RNA ligase

Physiological and Pharmacological Manipulations with Light Flashes

“…o-Nitrobenzyl ethers have also been prepared and photolyzed to yield hydroxyl groups in phenols (6), sugars (8,116,117), and nucleotides 22,83,84). Both the amino and hydroxy groups can be photogenerated in aqueous solution, but nothing is known about the speed of the reactions.…”

“…In a conceptual sense, this chapter could treat flash-photolysis investigations of heme proteins; but the topic has recently been reviewed authoritatively (84). This review does not concern photoaffinity labeling (28) or studies on fluorescence recovery after photobleaehing.…”

Chemistry of Nucleosides and Nucleotides

“…Ohtsuka el al. (365) used l-benzoyl-2'-O-tetrahydropyranyl-lţI-uridine in their synthesis of the trinucleotide, rTplţlpC. Brown el al.…”

Studies on transfer ribonucleic acids and related compounds. XXXII. Synthesis of ribonucleotides corresponding to residues 1-5 and 6-10 of tRNAfMetfrom E.coli and their base conversion analogs

“…We have previously reported chemical synthesis of E.coli URNAfMet 2 fragments by phosphodiester methods (bases 1-4,3 [5][6][7][8][9][10]4 41-46,5 47-54,5 55-57,5 58-60,6 61-65,7 66-71,7 72-74,8 75-77, 8,9 or a phosphotriester method (bases 11-20,10 72_77,1 and its analogs.…”

“…The phosphotriester approach using 2'-O-(o-nitrobenzylnucleoside)3'-phosphorodianilidates was also employed in the synthesis of a eukaryotic initiator tRNA loop IV heptanucleotide.12 In the present paper we report a synthesis of 5'pentanucleotides from E.coli tRNAfMet, C-G-C-G-Gp (bases [1][2][3][4][5], U-G-C-G-Gp (base 1 transition analog), pG-G-C-G-Gp (base 1 transversion analog) and pG-G-s4U-G-Gp (bases 6-10) using 2'-O-(onitrobenzyl nucleoside)-3'-p-chlorophenylphosphoranilidates.10' 13 Protected pGp was prepared for insertion of the 5'-phosphomonoester in the synthesis of the pentanucleotide, pG-G-C-G-Gp and the pentanucleotide was converted to the s4U containing oligonucleotide by treatment with liquid hydrogen sulfide.15 Synthesis of C-G-C-G-Gp (10) and U-G-C-G-Gp (11) For the synthesis of pentanucleotides (8,9), a common intermediate tetranucleotide (5) was prepared by condensation of two a InfnrM Retival Umed I Falconb.rg Court London W1VSFG England Nucleic Acids Research…”