Synthesis of enantiomerically pure α-[14C]methyl-L-tryptophan

Mzengeza, Shadreck; Venkatachalam, T. K.; Rajagopal, Srinivasan; Dikšić, Mirko

doi:10.1016/0969-8043(93)90059-j

Cited by 38 publications

(19 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…All of the minipumps were checked for effectiveness by examining whether they were empty of their contents at the end of each study. α-[ 14 C]MTrp was synthesized using a previously described procedure (Mzengeza et al 1993). It had a specific activity of ∼55 mCi mmol −1 .…”

Section: Drugmentioning

confidence: 99%

Chronic administration of citalopram in olfactory bulbectomy rats restores brain 5-HT synthesis rates: an autoradiographic study

et al. 2004

View full text Add to dashboard Cite

There is no significant global or individual structure difference in the synthesis between the Sham-CTP and OBX-CTP groups. The similarity in the synthesis between the OBX-CTP, Sham-CTP and Sham-SAL groups is likely a result of changes in the sensitivity of the receptors through which 5-HT synthesis is controlled. Because of some of the differences in the synthesis between the Sham-CTP and Sham-SAL groups, the data suggest that receptors throughout the brain are not fully desensitized.

show abstract

Section: Drugmentioning

confidence: 99%

Chronic administration of citalopram in olfactory bulbectomy rats restores brain 5-HT synthesis rates: an autoradiographic study

et al. 2004

View full text Add to dashboard Cite

show abstract

“…Reserpine and NSD-1015 were prepared freshly before injections. a-[ 14 C]Methyl-L-tryptophan (specific activity of about 55 mCi/mmol) was synthesized by us using the procedure described by Mzengeza et al (1993).…”

Section: Drugsmentioning

confidence: 99%

The Acute Effects of Reserpine and NSD-1015 on the Brain Serotonin Synthesis Rate Measured by an Autoradiographic Method

Mück‐Šeler

Dikšić

1995

Neuropsychopharmacology

View full text Add to dashboard Cite

The rate of serotonin (5-HT) synthesis was measured in the discrete regions of the rat brain utilizing an autoradiographic method and a[ 14 CJ methyl-L-tryptophan as a tracer after an acute treatment with reserpine (10 mg/kg IP) or NSD-1015 (m-hydroxybenzylhydrazine) (100 mg/kg IPIn the 1970s and the 1980s abundant investigations of the central serotoninergic system has led to the conclusion that serotonin (5-HT) plays a role in the regulation 1993 (abstract No. 126.18).

show abstract

“…The tracer, 30 μCi of α-14 C-methyl-L-tryptophan (α-[ 14 C] MTrp; specific activity of approximately 55 mCi/mmol; synthesized by us using the procedure described by Mzengeza et al [19]) was injected intravenously in 1 ml of saline over 2 min, with an injection pump. With the beginning of the tracer injection, arterial blood samples were taken at progressively increased time intervals up to the time the rats were sacrificed.…”

Section: Determination Of 5-ht Synthesis Ratementioning

confidence: 99%

Acute treatment with fluvoxamine elevates rat brain serotonin synthesis in some terminal regions: An autoradiographic study

Mück‐Šeler

Pivac

Dikšić

2012

Nuclear Medicine and Biology

View full text Add to dashboard Cite

Introduction-A considerable body of evidence indicates the involvement of the neurotransmitter serotonin (5-HT) in the pathogenesis and treatment of depression.Methods-The acute effect of fluvoxamine, on 5-HT synthesis rates was investigated in rat brain regions, using α-14 C-methyl-L-tryptophan as a tracer. Fluvoxamine (25 mg/kg) and saline (control) were injected intraperitoneally, one hour before the injection of the tracer (30 μCi).Results-There was no significant effect of fluvoxamine on plasma free tryptophan. After Benjamini-Hochberg False Discovery Rate correction, a significant decrease in the 5-HT synthesis rate in the fluvoxamine treated rats, was found in the raphe magnus (−32%), but not in the median (−14%) and dorsal (−3%) raphe nuclei. In the regions with serotonergic axon terminals, significant increases in synthesis rates were observed in the dorsal (+41%) and ventral (+43%) hippocampus, visual (+38%), auditory (+65%) and parietal (+37%) cortex, and the substantia nigra pars compacta (+56%). There were no significant changes in the 5-HT synthesis rates in the median (+11%) and lateral (+24%) part of the caudate-putamen, nucleus accumbens (+5%), VTA (+16%) or frontal cortex (+ 6%).Conclusions-The data show that the acute administration of fluvoxamine affects 5-HT synthesis rates in a regionally specific pattern, with a general elevation of the synthesis in the terminal regions and a reduction in some cell body structures. The reasons for the regional specific effect of fluvoxamine on 5-HT synthesis are unclear, but may be mediated by the presynaptic serotonergic autoreceptors. Keywords

show abstract

Synthesis of enantiomerically pure α-[14C]methyl-L-tryptophan

Cited by 38 publications

References 13 publications

Chronic administration of citalopram in olfactory bulbectomy rats restores brain 5-HT synthesis rates: an autoradiographic study

Chronic administration of citalopram in olfactory bulbectomy rats restores brain 5-HT synthesis rates: an autoradiographic study

The Acute Effects of Reserpine and NSD-1015 on the Brain Serotonin Synthesis Rate Measured by an Autoradiographic Method

Acute treatment with fluvoxamine elevates rat brain serotonin synthesis in some terminal regions: An autoradiographic study

Contact Info

Product

Resources

About