Synthesis of Encapsulated Chromolaena Odorata Leaf Extract in Chitosan Nanoparticle by Using Ionic Gelation Method and Its Antioxidant Activity

Idacahyati, Keni; Wulandari, Winda Trisna; INDRA, FIRMAN GUSTAMAN AND INDRA

doi:10.22159/ijap.2021.v13s4.43828

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…Therefore, LEChXg fulfilled the requirements as a nanoparticle with a size range be tween 50-500 nm particle (Ismail and Harun 2019). Ac cording to Idacahyati et al (2021), a nanosize particle should range from 1-1000 nm. PDI is a value that shows particle size distribution with a range of 0-1.…”

Section: Characteristics Of Lechxg Particlementioning

confidence: 99%

Chitosan Xylotrupes gideon encapsulated lemongrass leaf ethanol extract reduce H2O2‐induced oxidative stress in human dermal fibroblast

Komariah,

Trisfilha,

Wahyudi

et al. 2023

Indones. J. Biotechnol.

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During phagocytosis, phagocyte cells discharge reactive oxygen species referred to as respiratory bursts, inducing a rise in pro‐oxidants and subjecting the cell to oxidative stress. Such stress is a biological mechanism related to an imbalance in pro‐oxidant/antioxidant homeostasis, which generates toxic reactive oxygen. Encapsulation is a coating process to improve the stability of bioactive compounds from lemongrass extract. Therefore, this study aims to determine the encapsulation activity of lemongrass leaf extract with chitosan X. gideon (LEChXg) to reduce the oxidative stress of fibroblasts. The research used the human dermal fibroblast (HDF) cell line, comprising negative and positive controls and use of LEChXg 100, 200, 300, 400, and 500 µg/mL. HDF cell migration was evaluated by employing the scratch wound healing method and the wound closure was oberseved at 0, 2, 4, 6, and 24 h intervals. The cell proliferation was observed at 24, 48, and 72 h using CCK‐8 at a 450 nm wavelength. The results showed that the observations at 0, 2, and 4 h did not demonstrate any significant difference on the cell migration (p > 0.05) among the groups. However, the wound closure at 4 and 6 h showed a significant difference (p < 0.05) with LEChXg 300 µg/mL. Despite the lack of any significant variation observed up to 24 h, fibroblast subjected to the stressor did not achieve complete closure. The groups treated with LEChXg were more stable in maintaining fibroblast proliferation up to the end of the observation than those with stressors at 24, 48, and 72 h. Fibroblast induced with a stressor was also more stable in maintaining migration and proliferation in groups receiving LEChXg 300 µg/mL.

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Section: Characteristics Of Lechxg Particlementioning

confidence: 99%

Chitosan Xylotrupes gideon encapsulated lemongrass leaf ethanol extract reduce H2O2‐induced oxidative stress in human dermal fibroblast

Komariah,

Trisfilha,

Wahyudi

et al. 2023

Indones. J. Biotechnol.

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Formulation of Binahong Leaves (Anredera Cordifolia) Extract Nanoparticle and Evaluation of in Vivo Antidiabetic Activity

RAHMAT,

WALUYO

et al. 2024

Int J App Pharm

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Objective: Binahong leaves (Anredera cordifolia (Ten.) Steenis) is an herbal plant that is most commonly used to cure various types of diseases in Indonesia, including diabetes. The objective of this study was to conduct a comparative analysis of the antidiabetic activities between binahong leaves extract and binahong leaves extract nanoparticles. Methods: Nanoparticle formulation was carried out using chitosan and sodium alginate polymers as crosslinkers. DDY strain mice with 70% ethanol extract of binahong leaves as test material Binahong leaf extract was tested on alloxan-induced diabetic mice at a dose of 200 mg/kgBW intraperitoneally. The dose of binahong extract used was 200 mg/kgBB and 200 mg/kgBB of chitosan-sodium alginate nanoparticles orally for 14 ds. Results: Obtained extract nanoparticle met the specification, with average sizes of 192.7 nm, zeta potential of+25.3 mV. Extract nanoparticle with a dose equivalent to 200 mg/kgBB binahong leaf extract can reduce blood glucose levels in mice induced by alloxan was better than binahong leaf extract at a dose 200 mg/kgBW with respective percent reduction in blood glucose levels are 45.89% and 33.38%. Conclusion: The formulated extract nanoparticle can enhance the antidiabetic activity of binahong leaves extract.

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Synthesis of Encapsulated Chromolaena Odorata Leaf Extract in Chitosan Nanoparticle by Using Ionic Gelation Method and Its Antioxidant Activity

Cited by 2 publications

References 19 publications

Chitosan Xylotrupes gideon encapsulated lemongrass leaf ethanol extract reduce H2O2‐induced oxidative stress in human dermal fibroblast

Chitosan Xylotrupes gideon encapsulated lemongrass leaf ethanol extract reduce H2O2‐induced oxidative stress in human dermal fibroblast

Formulation of Binahong Leaves (Anredera Cordifolia) Extract Nanoparticle and Evaluation of in Vivo Antidiabetic Activity

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