Synthesis of fatty acid esters from acid oils using lipase B from <b><i>Candida antarctica</i></b>

Tüter, Melek; Aksoy, H. A.; Gılbaz, E. Elif; Kurşun, Emel

doi:10.1002/ejlt.200401007

Cited by 23 publications

(22 citation statements)

References 8 publications

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“…Many research groups have been searching for a reasonable source of FAME in addition to refined vegetable oil [1][2][3][4][5][6][7][8][9][10][11][12][13]. One of the promising candidates is acid oil.…”

Section: Introductionmentioning

confidence: 99%

Enzymatic Production of Fatty Acid Methyl Esters by Hydrolysis of Acid Oil Followed by Esterification

Watanabe

Nagao

Nishida

et al. 2007

J Americ Oil Chem Soc

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Acid oil, a by-product of vegetable oil refining, was enzymatically converted to fatty acid methyl esters (FAME). Acid oil contained free fatty acids (FFA), acylglycerols, and lipophilic compounds. First, acylglycerols (11 wt%) were hydrolyzed at 30°C by 20 units Candida rugosa lipase/g-mixture with 40 wt% water. The resulting oil layer containing 92 wt% FFA was used for the next reaction, methyl esterification of FFA to FAME by immobilized Candida antarctica lipase. A mixture of 66 wt% oil layer and 34 wt% methanol (5 mol for FFA) were shaken at 30°C with 1.0 wt% lipase. The degree of esterification reached 96% after 24 h. The resulting reaction mixture was then dehydrated and subjected to the second esterification that was conducted with 2.2 wt% methanol (5 mol for residual FFA) and 1.0 wt% immobilized lipase. The degree of esterification of residual FFA reached 44%. The degree increased successfully to 72% (total degree of esterification 99%) by conducting the reaction in the presence of 10 wt% glycerol, because water in the oil layer was attracted to the glycerol layer. Over 98% of total esterification was maintained, even though the first and the second esterification reactions were repeated every 24 h for 40 days. The enzymatic process comprising hydrolysis and methyl esterification produced an oil containing 91 wt% FAME, 1 wt% FFA, 1 wt% acylglycerols, and 7 wt% lipophilic compounds.

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Section: Introductionmentioning

confidence: 99%

Enzymatic Production of Fatty Acid Methyl Esters by Hydrolysis of Acid Oil Followed by Esterification

Watanabe

Nagao

Nishida

et al. 2007

J Americ Oil Chem Soc

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“…The conversion ratio to propyl oleate was almost same at 50 and 60°C when immobilized lipase was used. In contrast, Tuter et al (2004) reported that the optimum temperature range for C. antarctica was 50-60°C. The ester conversion at 30°C resulted in a mixture of the highest FAME contents, 50.1% for corn oil where as the highest FAME content of 63.6% for sunflower oil was obtained at 40°C and increasing the temperature to 60°C decreased the ester content.…”

Section: Effect Of Temperaturementioning

confidence: 84%

An overview on the recent advances in the transesterification of vegetable oils for biodiesel production using chemical and biocatalysts

Ganesan

Rajendran

Viruthagiri

2009

Rev Environ Sci Biotechnol

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Biodiesel, chemically defined as monoalkyl esters of long chain fatty acids, are derived from renewable feed stocks like vegetable oils and animal fats. It is produced by both batch and continuous transesterification processes in which, oil or fat is reacted with a monohydric alcohol in the presence of a catalyst. The conventional method of producing biodiesel involves acid and base catalysts to form fatty acid alkyl esters. Downstream processing costs and environmental problems associated with biodiesel production and byproducts recovery have led to the search for alternative production methods and alternative substrates. Enzymatic reactions involving lipases can be an excellent alternative to produce biodiesel through a process commonly referred to as alcoholysis, a form of transesterification reaction or through an interesterification reaction. In order to increase the cost effectiveness of the process, the enzymes are immobilized using a suitable matrix. The use of immobilized lipases and whole cells may lower the overall cost, while presenting less downstream processing problems. Main focus of this paper is to discuss the important parameters that affect the biodiesel yield, various immobilization techniques employed, mechanisms and kinetics of transesterification reaction and the recent advances in continuous transesterification processes.

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“…Tüter i sar. [33] su izveli esterifikaciju kiselih ulja kukuruza i suncokreta pomoću različitih alkohola (metanol, n-propanol, n-i izo-butanol, n-i izoamilalkohol i n-oktanol) i lipazom Novozym 435 u nheksanu. Primenom metanola ostvaren je prinos estara od 63,6 % za 1,5 h, dok je nešto veći prinos (oko 70 %) dobijen sa ostalim primarnim alkoholima.…”

Section: Enzimski Katalizovani Procesiunclassified

Side-stream products of edible oil refining as feedstocks in biodiesel production

Cvetković¹,

Cvetković²,

Kojčin³

et al. 2016

Rec odr raz

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IZVOD Biodizel, alternativno gorivo fosilnim, dobija se reakcijom transesterifikacije triacilglicerola iz biljnih ulja i životinjskih masti nižim alifatičnim alkoholima. I pored brojnih prednosti u odnosu na fosilna goriva, glavna prepreka široj komercijalnoj upotrebi biodizela je visoka cena jestivih biljnih ulja. U poslednje vreme, posebnu pažnju zaslužuju nusproizvodi industrije proizvodnje jestivih ulja kao izvori triacilglicerola, jer se njihovim korišćenjem ostvaruju pozitivni ekonomski i ekološki efekti. U radu su analizirani postupci dobijanja biodizela iz nusproizvoda procesa rafinacije jestivih ulja (sapunske smeše, otpadna zemlja za beljenje, deodorisani destilat), sa osvrtom na faktore koji utiču na reakciju sinteze estara masnih kiselina. Cilj rada je da se istraže mogućnosti korišćenja ovih otpadnih sirovina u proizvodnji biodizela.

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Synthesis of fatty acid esters from acid oils using lipase B from Candida antarctica

Cited by 23 publications

References 8 publications

Enzymatic Production of Fatty Acid Methyl Esters by Hydrolysis of Acid Oil Followed by Esterification

Enzymatic Production of Fatty Acid Methyl Esters by Hydrolysis of Acid Oil Followed by Esterification

An overview on the recent advances in the transesterification of vegetable oils for biodiesel production using chemical and biocatalysts

Side-stream products of edible oil refining as feedstocks in biodiesel production

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